Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effects of Extraction Time on the Quality Characteristics of Purple Corn Cob Extract

추출시간에 따른 자색 옥수수 속대 추출물의 품질특성

  • Jeong, Ji-yun (Department of Food Processing Technology, Division of Strategic Food Research) ;
  • Park, Hee-Jin (Department of Food Processing Technology, Division of Strategic Food Research) ;
  • Kim, Byung-Hee (Department of Food Science and Technology, Chung-ang University) ;
  • Kim, Sung-Soo (Department of Food Processing Technology, Division of Strategic Food Research)
  • Received : 2015.06.01
  • Accepted : 2015.09.07
  • Published : 2015.12.31
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Abstract

In this study, the quality characteristics of purple corn cob extract (PCCE) by extraction time were examined. The cob of purple corn, which is mainly cultivated in the Andes region of South America, contains more anthocyanin than the corresponding purple corn seeds. The aim of this study was to determine the optimal conditions for the preparation of water extracts from purple corn cob. PCCE had a soluble solid content of $0.6^{\circ}Brix$. The Hunter lightness ($L^*$), redness ($a^*$), yellowness ($b^*$) values of the PCCE were 31.0, 38.4, and 9.0, respectively. The PCCE contained 24.64 mg/g of cyanidin, 0.35 mg/g of pelargonidin, and 17.42 mg/g peonidin, and showed greater antioxidant activity than the other extracts. Therefore, the optimal extracting conditions for preparing PCCE were a temperature of $30^{\circ}C$ and an extraction time of 24 h.

본 연구는 자색 옥수수 속대의 가공식품 및 기능성 식품의 원료로서 이용 가능성을 평가하기 위하여 자색 옥수수의 속대 추출 시간을 달리하여 추출물을 제조하고 품질특성을 조사하였다. 추출 시간에 따른 자색 옥수수 속대 추출물 분석 결과, 자색 옥수수 속대를 $30^{\circ}C$에 24시간까지 추출하면서 시간의 경과에 따라 가용성 고형물 및 적색도의 증가와 명도 및 황색도의 감소가 나타났다. 그러나 추출 시간이 24시간을 경과함에 따라 품질 특성은 24시간 추출물과 유사하거나 가용성 고형물 및 적색도가 감소하였으며 명도 및 황색도가 증가하였다. 특히 항산화 활성은 추출 24시간까지 유의적으로 증가했으며 24시간 이후 유의적인 차이는 나타나지 않았다. 즉, 자색 옥수수 속대는 가용성 고형물 함량, 안토시아니딘 함량, 항산화활성이 24시간에서 유의적으로 높았기 때문에 자색 옥수수 속대의 최적 추출 시간은 24시간으로 판단되었다.

Keywords

References

  1. Benzie IF and Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. J Anal Biochem 239, 706.
  2. Blois MS (1958) Antioxidant determinations by the use or a stable free radical. Nature 181, 1990-2100.
  3. Chang HJ, Choi EH, and Chun HS (2008) Quatitative strcture-activity relationship (QSAR) of antioxidative anthocyanins and their glycosides. Food Sci Biotechnol 17, 501-7.
  4. Choi SW, Lee SK, Kim EO, Oh JH, Yoon KS, Parris N et al. (2007) Antioxidant and antimelanogenic activities of polyamine conjugates from corn bran and related hydroxycinnamic acids. J Agric Food Chem 23, 1090-2.
  5. Choung MG, Hwang YS, Lee HJ, Choi SSN, Lim JD, Kang ST et al. (2008) Optimal extraction condition of anthocyanins in soybean (Glycine max) with black seed coats. Kor J Crop Sci 53, 110-7.
  6. Coe Jr. EH, Neuffer MG, and Hoisington DA (1988) The genetics of corn. In Corn and Corn Improvement, Spague GF and Dudley JW (3rd ed.), pp. 83-258. American Society of Agronomy, USA.
  7. Dey PM and Harborne JB (1993) In Plant phenolics methods in plant biochemistry, (2nd ed.). Academic Press Ltd, UK.
  8. Henry BS (1992) Natural food colors. In Natural Food Colorants, Hendry GAF and Houghton JD (eds), pp.39. Blackie and Son Ltd., Scotland.
  9. Heo HJ and Lee CY (2005) Strawberry and its anthocyanins reduce oxidative stress-induced apoptosis in PC12 cells. J Agric Food Chem 53, 1984-9. https://doi.org/10.1021/jf048616l
  10. Ichikawa H, Ichiyanagi T, Xu B, Yoshii Y, Nakajima M, and Konishi T (2001) Antioxidant activity of anthocyanin extract from purple black rice. J Medicinal Food 4, 211-8. https://doi.org/10.1089/10966200152744481
  11. Jakobek L, Seruge M, Medvidovic-Kosanovic M, and Novak I (2007) Anthocyanin content and antioxidant activity of various red fruit juices. Deutsche Lebensmittel-Rundschau 103, 58-64.
  12. Jing P and Giusti MM (2007) Effects of extraction conditions on improving the yield and quality of an anthocyanin-rich purple corn (Zea mays L.) color extract. J Food Sci 72, 363-8.
  13. Kenji H, Bayaru E, Hiroki M, and Shigeru S (2009) Silage fermentatice quality and characteristics of anthocyanin stability in anthocyanin-rich corn (Zea mays L.). Asian-Aust J Anim Sci 22, 528-33. https://doi.org/10.5713/ajas.2009.80525
  14. Kim BS, Park YK, and Kang BS (2001) The effect of Rubi fructus on the ovulation and ovary in rats. Kor J Herb 16, 139-52.
  15. Kim JT, Son BY, Lee JS, Baek SB, Kim SL, Kim MJ et al. (2013) National dyeing fabrics with leaf and stem of purple corn. Kor J Crop Sci 58, 113-8. https://doi.org/10.7740/kjcs.2013.58.2.113
  16. Lachman J, Hanouz K, Sulc M, Orsak M, Pivec V, Hejtmankova A et al. (2009) Cultivar differences of total anthocyains and anthocyanidins in red and purple fleshed potatoes and their relation to antioxidant activity. Food Chemistry 114, 83643.
  17. Lee HJ, Jang JS, Choi EY, and Kim YH (2008) Anthocyanin content and color stability in black rice according to different extract conditions and selected stabilizers. Kor J Food Nutr 21, 127-34.
  18. Li CY (2007) Antioxidant effect of anthocyanins from purple corn (Zea mays L.) and its application to food. MS thesis, Kangwon National University, Korea.
  19. Lopez-Martinez LX, Oliart-Ros RM, Valerio-Alfaro G, Lee CH, Parkin KL, and Garcia HS (2009) Antioxidant activity, phenolic compounds and anthoctanins content of eighteen strains of Mexican maize. LWT-Food Sci Technol 42, 1187-92. https://doi.org/10.1016/j.lwt.2008.10.010
  20. Malaj N, Simone BCD, Quartarolo AD, and Russo N (2013) Spectrophotometric study of the copigmentation of malvidin 3-Oglucoside with p-coumaric, vanillic and syringic acids. Food Chem 141, 3614-20. https://doi.org/10.1016/j.foodchem.2013.06.017
  21. Mazza G and Miniati E (1993) In Anthocyanin in fruits, vegetables and grains, CRC Press, UK.
  22. Meiers S, Kemeny M, Weyand U, Gastpar R, Von Angerer E, and Marko D (2001) The anthocyanidins cyanidin and delphinidin are potent inhibitors of the epidermal growth-factor receptor. J Agric Food Chem 49, 958-62. https://doi.org/10.1021/jf0009100
  23. Mellon JE and Moreau RA (2004) Inhibition of aflatoxin biosynthesis in Aspergillus flavus by diferuloylputrescine and p-coumaroylferuloylputrescine. J Agric Food Chem 52, 6660-3. https://doi.org/10.1021/jf040226b
  24. Montes C, Vicario IM, Raymundo M, Fett R, and Heredia FJ (2005) Application of tristimulus colourimetry to optimize the extraction of anthocyanin from Jaboticaba (My ricia Jaboricab Berg.). Food Res Int 38, 983-8. https://doi.org/10.1016/j.foodres.2005.01.016
  25. Moyer RA, Hummer KE, Finn CE, Frei B, and Wrolstad RE (2002) Anthocyanins, phenolics and antioxidant capacity in diverse small fruits: vaccinium, rubus, and ribes. J Agric Food Chem 50, 519-25. https://doi.org/10.1021/jf011062r
  26. Niwa T, Doi U, and Osawa T (2003) Inhibitory activity of cornderived bisamide compounds against $\alpha$-glucosidase. J Agric Food Chem 51, 90-4. https://doi.org/10.1021/jf020758x
  27. Pavel S, Booivoj K, and Vlastimil K (2006) Determination of total content of phenolic compounds and their antioxidant activity in vegetables-Evaluation of spectrophotometric methods. J Agric Food Chem 54, 60716.
  28. Plate AYA and Gallaher DD (2005) The potential health benefits of corn components and products. Cereal Foods World 50, 305-14.
  29. Rivero-Prez MD, Muniz P, and Gonzalez-Sanjose ML (2008) Contribution of anthocyanin fraction to the antioxidant properties of wine. Food and Chemical Toxicology 46, 281522.
  30. Seeram NP and Nair MG (2002) Inhibition of lipid peroxidation and structure-activity-related studies of the dietary constituents anthocyanins, anthocyanidins. And catechins. J Agric Food Chem 50, 5308-12. https://doi.org/10.1021/jf025671q
  31. Smith MA, Rottkamp A, Nunomura A, Raina AK, and Perry G (2000) Oxidative stress in Alzheimer's disease. Biochim Biophys Acta 1502, 139-44. https://doi.org/10.1016/S0925-4439(00)00040-5
  32. Tsuda T, Shiga K, Ohshima K, Kawakishi S, and Osawa T (1996) Inhibition of lipid peroxidation and the active oxygen radical scavenging effect of anthocyanin pigment isolated from Phaseolus Vulgaris L. Biochem Pharmacol 52, 1033-9. https://doi.org/10.1016/0006-2952(96)00421-2
  33. Tsuda T, Watanabe M, Ohshima K, Norinobu S, Choi SW, Kawakishi S et al. (1994) Antioxidative activity of the anthocyanin pigments cyanidin 3-O-$\beta$-D-glucoside and cyanidin. J Agric Food Chem 42, 2407-10. https://doi.org/10.1021/jf00047a009
  34. Tusba T, Horio F, Uchida K, Akoi H, and Osawa T (2003) Dietary cyanidin 3-O-$\beta$-D-glucoside-rich purple corn color prevents obesity and ameliorates hyperglycemia in mice. J Nutr 133, 2125-35. https://doi.org/10.1093/jn/133.7.2125
  35. Yang Z and Zhai W (2010) Identification and antioxidant activity of anthoctanins extracted from the seed and cob of purple corn (Zea mays L.) Innov Sci Emerg Technol 11, 169-76. https://doi.org/10.1016/j.ifset.2009.08.012
  36. Yang Z, Chen Z, Yuan S, Zhai W, Piao X, and Piao X (2009) Extraction and identification of anthocyanin from purple corn (Zea Mays L.). Int J Food Sci Technol 44, 2485-92. https://doi.org/10.1111/j.1365-2621.2009.02045.x

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