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Loperamide로 유도된 변비 증상에 유산균 제제가 미치는 영향

Effect of Lactic Acid Bacteria Powder on Loperamide-induced Constipation in Rat

  • 김은영 (고려대학교 대학원 보건과학과) ;
  • 조경애 (고려대학교 대학원 보건과학과) ;
  • 안소현 (고려대학교 대학원 보건과학과) ;
  • 박성선 (종근당건강) ;
  • 손흥수 (신안산대학교 식품생명과학과) ;
  • 한성희 (고려대학교 생물신소재연구소)
  • Kim, Eun Young (Dept. of Public Health Sciences, Graduate School, Korea University) ;
  • Jo, Kyungae (Dept. of Public Health Sciences, Graduate School, Korea University) ;
  • Ahn, So Hyun (Dept. of Public Health Sciences, Graduate School, Korea University) ;
  • Park, Sung Sun (Research Center, Chong Kun Dang Healthcare Corporation) ;
  • Son, Heung Soo (Dept. of Food Science and Biotechnology, Shin Ansan University) ;
  • Han, Sung Hee (Institute for Biomaterials, Korea University)
  • 투고 : 2015.10.19
  • 심사 : 2015.11.28
  • 발행 : 2015.12.31

초록

Loperamide를 통해 변비가 유발된 실험동물에 유산균 투여가 미치는 영향을 알아보기 위하여 14일 동안 저농도($10^7CFU/mL$ per kg of body weight)의 유산균과 고농도($10^9CFU/mL$ per kg of body weight)의 유산균을 투여한 후, 변비개선 효과를 측정하였다. 그 결과, 변비 유발 후 유산균을 투여한 실험군들의 경우, 대조군(CON)에 비하여 변의 개수, 변 중량 및 수분 함량이 유의적으로 증가하였다. 또한 소화관 이동률을 조사한 결과, 대조군(CON)에 비하여 변비 유발 후 유산균을 투여한 군에서 정상대조군(NOR)인 군과 유사한 이동성을 보이는 것을 확인하였다. 변비가 유발된 실험동물의 혈청 중성지방, 총 콜레스테롤 함량, HDL-콜레스테롤 함량에는 유산균 투여 유무 및 유산균의 농도에 대한 영향을 크게 받지 않은 것으로 보여진다. 고농도의 유산균을 섭취시킨 실험동물군(HIG)의 경우, 분변으로 유도된 아세트산과 프로피온산의 함량이 유의적으로 높게 나타났다. 또한 헤마톡실린 및 에오신 염색을 통한 장내 상피세포 관찰을 통해 유산균 분말 투여시 장 점막의 길이와 넓이가 유의적으로 증가하는 것을 확인하였다. 메타지노믹스 유전자 분석을 통한 미생물 분포의 상동성을 비교해 본 결과, 고농도의 유산균 투여군(HIG)이 정상대조군(NOR)과 가장 흡사한 분포 특성을 가지고 있음을 보여주었다. 이상의 결과에 따라 유산균 제제는 변비 개선 효과가 있다고 볼 수 있다.

Probiotics is known improve the microenvironment of colon; however, the metagenomic DNA study of its lactic acid bacteria in constipation induced by loperamide is not clearly understood. In the present study, we investigated the reduction of the lactic acid bacteria in case of constipation, in normal and loperamide-induced rat. Lactic acid powder (lactic acid bacteria 19) was prepared from Chong Kun Dang Pharmaceutical Corporation. After 2 weeks of oral administration, the group treated with the higher concentration of lactic acid bacteria ($10^9CFU/mL$ per kg of body weight) following loperamide treatment was the most effective in increasing number, weight, and water content of feces. A similar but significant increase was found in the group treated with lower concentration of lactic acid bacteria ($10^7CFU/mL$ per kg of body weight) after loperamide treatment. The concentrations of acetic acid and propionic acid in feces in the loperamide-induced rat with high concentration lactic acid, were significantly higher than that of others. Furthermore, gastrointestinal transit ratio as well as the length and area of intestinal mucosa were significantly increased after treatment with lactic acid bacteria in loperamide-induced rat. Metagenomics DNA analysis indicated that the microorganism homology in cecum was similar between the groups of normal (NOR) and HIG. Our results show that lactic acid bacteria were effective in improving the constipation.

키워드

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피인용 문헌

  1. Effects of Fermented Milk with Mixed Strains as a Probiotic on the Inhibition of Loperamide-Induced Constipation vol.37, pp.6, 2017, https://doi.org/10.5851/kosfa.2017.37.6.906
  2. Amylase-Producing Maltooligosaccharide Provides Potential Relief in Rats with Loperamide-Induced Constipation vol.2020, pp.None, 2015, https://doi.org/10.1155/2020/5470268
  3. 유산균제제(Lactobacillus helveticus HY7801)의 경구 투여가 아토피 피부염 동물모델의 면역 반응 및 피부 개선에 미치는 효과 vol.33, pp.2, 2020, https://doi.org/10.9799/ksfan.2020.33.2.174
  4. Bifidobacterium longum과 자일로올리고당을 포함한 synbiotics 섭취가 건강한 성인의 변내 균총과 배변 상태에 미치는 영향 vol.53, pp.4, 2015, https://doi.org/10.4163/jnh.2020.53.4.390