Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Inhibitory Effect of Ginkgo biloba Extracts on Melanin Biosynthesis

은행 열매 추출물의 멜라닌 생성 저해효과

  • Kim, Yoon Suk (Department of Herbal Cosmetics Science, Reasearch institute for basic Sciences, Hoseo University) ;
  • Lee, Young Hwa (Department of Herbal Cosmetics Science, Reasearch institute for basic Sciences, Hoseo University) ;
  • Lee, Jin Young (Department of Herbal Cosmetics Science, Reasearch institute for basic Sciences, Hoseo University) ;
  • Yi, Yong sub (Department of Herbal Cosmetics Science, Reasearch institute for basic Sciences, Hoseo University)
  • 김윤석 (호서대학교 한방화장품학과 기초과학연구소) ;
  • 이용화 (호서대학교 한방화장품학과 기초과학연구소) ;
  • 이진영 (호서대학교 한방화장품학과 기초과학연구소) ;
  • 이용섭 (호서대학교 한방화장품학과 기초과학연구소)
  • Received : 2015.11.19
  • Accepted : 2015.12.15
  • Published : 2015.12.30
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Abstract

In this study, we investigated the inhibitory effect on melanin synthesis of Ginkgo biloba seed oil. The results showed 9.96% inhibitory effect scavenging activity on DPPH and showed a value of 1.33 mM of $FeSO_4$ at a concentration of 0.06% in DMSO by using FRAP assay. G. biloba seed oil inhibited tyrosinase activity up tp 37.72% and suppressed the biosynthesis melanin up to 48.02% at 0.06% in B16/F10 mouse melanoma cell. In G. biloba seed oil treated group tyrosinase, TRP-1, TRP-2 and MITF gen expression levels significantly decreased compared to the contral group at a concentration of 0.04% and 0.06%. In conclusion, these results indicated that G. biloba seed oil extract have a good antimelanogenetic effects.

본 연구는 은행 열매 오일의 멜라닌 생성 억제 효과를 확인한 것이다. 은행나무 열매 오일은 DPPH assay와 FRAP assay를 사용하여 라디컬 소거능을 시험하였다. 결과적으로 은행나무 열매 오일은 DMSO를 용매로 0.06% 녹였을 때, DPPH assay에서 9.96% 소거활성을 나타내었고 FRAP는 1.33 mM의 ferric sulfate ($FeSO_4$)를 생성하였다. 은행 열매 오일은 tyrosinase inhibition assay에서 37.72%의 억제력을 가졌고 B16/F10 세포에 멜라닌 생합성 실험을 통해 확인하였다. 은행 오일 0.06%에서 ${\alpha}$-MSH 처리 구에 비해 48.02%의 멜라닌 생성을 억제하였다. Tyrosinase, tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2), microphthalmia-associated transcription factor (MITF)의 유전자 발현 수준은 control군에 비해 0.04%와 0.06% 농도 군이 크게 감소하였다. 결과적으로 은행 열매 오일 추출물이 멜라닌 생성을 억제하는 효과가 있는 것으로 확인되었다.

Keywords

References

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