Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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Biologic Response of Human Deciduous Dental Pulp Cells on Newly Developed MTA-like Materials

새로 개발된 MTA 유사 재료에 대한 유치 치수세포의 생물학적 반응

  • Lee, Haewon (Department of Pediatric Dentistry, College of Dentistry, Yonsei University) ;
  • Shin, Yooseok (Department of Conservative Dentistry, College of Dentistry, Yonsei University) ;
  • Jung, Jaeeun (Oral Science Research Center, College of Dentistry, Yonsei University) ;
  • Kim, Seongoh (Department of Pediatric Dentistry, College of Dentistry, Yonsei University) ;
  • Lee, Jaeho (Department of Pediatric Dentistry, College of Dentistry, Yonsei University) ;
  • Song, Jeseon (Department of Pediatric Dentistry, College of Dentistry, Yonsei University)
  • 이혜원 (연세대학교 치과대학 소아치과학교실) ;
  • 신유석 (연세대학교 치과대학 보존과학교실) ;
  • 정재은 (연세대학교 치과대학 구강과학연구소) ;
  • 김성오 (연세대학교 치과대학 소아치과학교실) ;
  • 이제호 (연세대학교 치과대학 소아치과학교실) ;
  • 송제선 (연세대학교 치과대학 소아치과학교실)
  • Received : 2015.03.23
  • Accepted : 2015.07.07
  • Published : 2015.11.30
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Abstract

This study compared the in vitro cell viability and differentiation potentials of human deciduous dental pulp cells (DPCs) on mineral trioxide aggregate (MTA)-like products (ProRoot MTA, RetroMTA and Endocem Zr). The experimental materials were prepared as circular discs, which were used to test the effects of the materials on the viability of human DPCs when placed in direct and indirect contact. Furthermore, the pH of the extracted materials was recorded, and their effect on cell differentiation potential was evaluated by evaluating the alkaline phosphatase (ALP) activity and Alizarin Red S staining of DPCs incubated with the test materials. In direct contact, the cell viability of human DPCs was higher with ProRoot MTA and RetroMTA than with Endocem Zr. However, when in indirect contact, the cell viability of human DPCs was generally higher in Endocem Zr than in ProRoot MTA and Retro MTA. With respect to pH, the alkalinity was lower for Endocem Zr than for the other test materials. The ALP activities of the cells were not enhanced by any of the experimental materials. Alizarin Red S staining of the tested human DPCs revealed that their differentiation potential was lower than for cells incubated with osteogenic induction medium. While there were differences in the responses of the human DPCs to the test materials, all displayed degrees of cytotoxicity and were unable to enhance either the viability or differentiation of human DPCs. However, Endocem Zr exhibited better cell viability and was less alkaline than the other test materials.

본 연구의 목적은 다양한 MTA 재료(ProRoot MTA, RetroMTA, Endocem Zr)에 대한 유치 치수 세포의 생활력 및 분화능을 비교 평가하는 것이다. 유치 치수세포는 각 재료별로 경화된 원형 디스크를 이용하여 직접법 및 간접법으로 세포 생활력을 관찰하였다. 또한 재료별 추출물을 이용하여 pH를 측정하였으며, alkaline phosphatase(ALP) 활성도 및 Alizarin Red S 염색법을 통하여 세포의 분화능을 관찰하였다. 직접법에서 유치 치수세포는 ProRoot MTA와 RetroMTA에서 Endocem Zr에 비해 높은 세포 생활력을 보였으나, 반면 간접법에서는 Endocem Zr에서 다른 재료에 비해 높은 세포 생활력이 관찰되었다. pH의 경우 Endocem Zr가 다른 두 재료에 비해 낮은 알칼리성을 나타냈다. 모든 재료에서 ALP 활성도는 대조군에 비해 증가하지 않았으며, Alizarin Red S 염색결과 유치 치수세포의 분화능이 대조군에 비해 낮았다. 본 실험에서 재료별 차이는 있었으나 모든 재료에서 어느 정도의 세포 독성이 관찰되었으며, 유치 치수세포의 생활력과 분화능을 증진시키지 못하였다. 하지만 Endocem Zr의 경우 ProRoot MTA나 RetroMTA에 비해 낮은 알칼리성과 높은 생활력을 보였다.

Keywords

References

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