Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Transcriptome Analysis of Human HaCaT Keratinicytes by Ginsenosides Rb1 and Rg1

진세노사이드 Rb1과 Rg1에 의한 HaCaT 피부각질세포의 전사체 분석

  • Kim, Jung Min (NAR Center, Inc., Daejeon Oriental Hospital of Daejeon University) ;
  • Cho, Won June (Dept. of Biological Science and the Research Institute for Basic Sciences, Hoseo University) ;
  • Yoon, Hee Seung (Dept. of Biological Science and the Research Institute for Basic Sciences, Hoseo University) ;
  • Bang, In Seok (Dept. of Biological Science and the Research Institute for Basic Sciences, Hoseo University)
  • 김정민 ((주)엔에이알센터 DNA칩 분석팀) ;
  • 조원준 (호서대학교 생명과학과) ;
  • 윤희승 (호서대학교 생명과학과) ;
  • 방인석 (호서대학교 생명과학과)
  • Received : 2014.10.27
  • Accepted : 2014.11.06
  • Published : 2014.11.30
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Abstract

This study examined the efficacy and the mechanism of action of biological response modifiers, ginsenosides Rb1 and Rg1 isolated from Panax ginseng C.A. Meyer on human keratinocytes HaCaT cell lines. A non-significant cytotoxic response was obtained in the HaCaT cell lines on treatment with various concentrations of ginsenosides Rb1 and Rg1 for different time durations. Furthermore, the global changes in the mRNA profile of HaCaT cells were investigated using DNA microarrays after stimulation with the ginsenosides Rb1 and Rg1. Ginsenosides Rb1 and Rg1 strongly increased FGF2 in HaCaT cells, and were found to be a candidate gene for antioxidant activity and elasticity. Other key candidate genes for antioxidant activity, such as FANCD2, LEPR, and FAS, also show enhanced regulation in HaCaT cells treated with ginsenoside Rb1. This study will be useful for understanding the regulatory genes involved in skin elasticity and signal transduction pathway stimulated by the ginsenoside Rb1. This paper currently focuses on the key factors regulating the interaction of anti-aging principles and skin elasticity.

인삼(Panax ginseng C. A. Meyer)의 주요 생리활성물질인 진세노사이드(ginsenoside) Rb1과 Rg1의 효능검증 및 작용점을 규명하고자 HaCaT 피부각질세포에서 유전체 분석(gene expression profiles)을 실시하였다. 진세노사이드 Rb1과 Rg1 각각의 처리 농도 및 시간에 따른 HaCaT 세포에 대한 세포독성은 나타나지 않았으며, $10{\mu}g/mL$의 진세노사이드 Rb1과 Rg1 각각을 6 및 24 시간 처리하여 유전체 분석 결과, 진세노사이드 Rb1과 Rg1의 24 시간 처리군에서 항노화 및 피부탄력 관련 유전자인 fibroblast growth factor (FGF2)의 활성이 증가된 것으로 나타났다. 또한 진세노사이드 Rb1의 24 시간 처리군에서는 항산화 작용점에 있는 일련의 유전자군, FANCD2, FGF2, LEPR, FAS 등의 활성을 확인하였다. 향후 확인된 항노화 및 피부탄력 관련 주요인자들의 작용 및 상관관계를 구체적으로 확인하고, 특히 진세노사이드 Rb1의 신호전달을 완성하고자 한다.

Keywords

References

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