Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Optimization of Medium for Carotenoids Production by Arthrobacter sp. PAMC 25486 Using Response Surface Methodology

반응표면분석법을 이용한 Arthrobacter sp. PAMC 25486의 카로티노이드 생산배지 최적화

  • Kim, Hyun-Do (Department of Biotechnology and Bioengineering, Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University) ;
  • Choi, Jong-Il (Department of Biotechnology and Bioengineering, Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University) ;
  • Han, Se Jong (Division of Life Sciences, Korea Polar Research Institute)
  • 김현도 (전남대학교 생물공학과 바이오에너지 및 바이오소재 협동과정) ;
  • 최종일 (전남대학교 생물공학과 바이오에너지 및 바이오소재 협동과정) ;
  • 한세종 (극지연구소 극지생명과학연구부)
  • Received : 2014.04.09
  • Accepted : 2014.05.18
  • Published : 2014.12.01
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Abstract

This study was conducted to optimize the medium composition for carotenoid production in Arthrobacter sp. PAMC 25486 through response surface methodology (RSM). Using a Placket-Burman design, from which yeast extract, $MgSO_4$ and dextrose were identified as the significant factors affecting carotenoids production. RSM studies for carotenoids production by Arthrobacter sp. PAMC 25486 have been carried out for three parameters of yeast extract, $MgSO_4$ and dextrose concentrations. These significant factors were optimized by experiments and RSM, as 1 g/L yeast extract, 0.0879 g/L $MgSO_4$ and 1 g/L dextrose. The experimentally obtained concentration of carotenoid was 288 mg/L, and it became 2-fold increase on concentration before optimization.

본 논문에서는 반응표면분석법을 이용한 Arthrobacter sp. PAMC 25486의 carotenoids 생산 배지의 최적화를 수행하였다. Placket-Burman 방법을 이용하여 yeast extract, $MgSO_4$, dextrose가 carotenoids의 생산에 영향을 미치는 주요인자인 것을 확인하였다. 반응표면분석 방법을 이용하여 최대 carotenoids 생산 농도를 갖는 yeast extract, $MgSO_4$, dextrose의 농도를 계산한 결과 1 g/L yeast extract, 0.0879 g/L $MgSO_4$ and 1 g/L dextrose의 농도에서 최대 307 mg/L의 carotenoids 농도가 예측됐으며, 실제 배양 결과 288 mg/L carotenoids가 얻어졌다. 얻어진 농도 값은 최적화 이전의 값에 비하여 200% 이상 증가하였다. 이러한 결과로부터 미생물 배양에 의한 carotenoids 생산을 증가시키기 위한 배지최적화 방법으로서 반응표면분석법의 유용성을 확인할 수 있었다.

Keywords

References

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