Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Transplantation and Production of OPU Derived Hanwoo IVP Embryos

OPU 유래 한우 수정란 생산 및 이식

  • Jin, Jong-In (Department of Animal Science, Division of Applied Life Science (BK21Plus Program)) ;
  • Choi, Byung-Hyun (Department of Animal Science, Division of Applied Life Science (BK21Plus Program)) ;
  • Kim, Seong-Su (Department of Animal Science, Division of Applied Life Science (BK21Plus Program)) ;
  • Jo, Hyun-Tae (Department of Animal Science, Division of Applied Life Science (BK21Plus Program)) ;
  • Sun, Du-Won (Department of Animal Science, Division of Applied Life Science (BK21Plus Program)) ;
  • Lim, Hyun-Tae (Department of Animal Science, Division of Applied Life Science (BK21Plus Program)) ;
  • Lee, Jung-Gyu (Department of Animal Science, Division of Applied Life Science (BK21Plus Program)) ;
  • Min, Chan-Sik (Gyeongsangnamdo Agriculture Research & Extension Services) ;
  • Kong, Il-Keun (Department of Animal Science, Division of Applied Life Science (BK21Plus Program))
  • 진종인 (경상대학교 응용생명과학부 축산학과) ;
  • 최병현 (경상대학교 응용생명과학부 축산학과) ;
  • 김성수 (경상대학교 응용생명과학부 축산학과) ;
  • 조현태 (경상대학교 응용생명과학부 축산학과) ;
  • 선두원 (경상대학교 응용생명과학부 축산학과) ;
  • 임현태 (경상대학교 응용생명과학부 축산학과) ;
  • 이정규 (경상대학교 응용생명과학부 축산학과) ;
  • 민찬식 (경상남도 농업기술원) ;
  • 공일근 (경상대학교 응용생명과학부 축산학과)
  • Received : 2014.09.05
  • Accepted : 2014.09.23
  • Published : 2014.09.30
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Abstract

This study was carried out to establish the system of OPU derived embryo production, management of recipients as well as offspring production. OPU derived embryo production system was carried out of aspiration of immature oocytes 2 times per week, total 24 times for 3 months by an ultrasonographic guided follicular aspiration system and then produced in vitro-produced blastocysts by in vitro maturation, fertilization and culture system. This work was collected total 13,866 oocytes, average $8.2{\pm}4.5$ oocytes per session and 8,170 G1 + G2 grade oocytes, average 4.8 oocytes per session by 1,692 times session of total 71 donors for 4 years from 2010 to 2013. The rate of cleavage and blastocyst developmental competence were obtained 11,825 (85.3%) and 5,032 (36.3%) that was $7.0{\pm}3.8$ cleaved embryos and $3.0{\pm}2.5$ blastocysts per session. OPU derived embryo transfer were taken place in 2, 4, 6 and 7 local governments at 2010, 2011, 2012 and 2013 for 4 years and pregnancy rate were obtained 41.2, 43.9, 46.5 and 49.7% in each years. It means that pregnancy rate was continuously improved according of every year for 4 years. Pregnancy rate was significantly different according to individual local government in which was 62.7% in B, but 24.2% in F at 2012. Paternity identification was carried out total 26 offspring in C local government of 2012 and then confirmed 100% agreement of its analysis. In conclusion, the results obtained the possibility of mass production of elite cow embryos as well as offspring by OPU derived embryo production system, of which could be decreased the required time of genetic improvement.

Keywords

References

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