Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of Collection Seasons on the Oocyte Quality and Developmental Competence of Oocytes Derived from Korean Native Cows (Hanwoo) by Ovum Pick-Up

OPU 채란계절이 한우의 난자 품질 및 발달 능력에 미치는 영향

  • Kim, Seong-Su (Department of Animal Science, Division of Applied Life Science (BK21Plus)) ;
  • Choi, Byung Hyun (Department of Animal Science, Division of Applied Life Science (BK21Plus)) ;
  • Jo, Hyun-Tae (Department of Animal Science, Division of Applied Life Science (BK21Plus)) ;
  • Jin, Jong-In (Department of Animal Science, Division of Applied Life Science (BK21Plus)) ;
  • Ha, A-Na (Department of Animal Science, Division of Applied Life Science (BK21Plus)) ;
  • Min, Chan-Sik (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Cho, Gyu-Wan (Colleage of Veterinary Medicine, Gyeongsang National University) ;
  • Kong, Il-Keun (Department of Animal Science, Division of Applied Life Science (BK21Plus))
  • 김성수 (경상대학교 응용생명과학부 축산학전공) ;
  • 최병현 (경상대학교 응용생명과학부 축산학전공) ;
  • 조현태 (경상대학교 응용생명과학부 축산학전공) ;
  • 진종인 (경상대학교 응용생명과학부 축산학전공) ;
  • 하아나 (경상대학교 응용생명과학부 축산학전공) ;
  • 민찬식 (경상남도 농업기술원) ;
  • 조규완 (경상대학교 수의과대학) ;
  • 공일근 (경상대학교 응용생명과학부 축산학전공)
  • Received : 2014.09.05
  • Accepted : 2014.09.23
  • Published : 2014.09.30
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Abstract

Implementation of smart embryo technologies in cattle e.g. ovum pick-up followed by in vitro embryo production (OPU-IVP). Seasonal variation is important factor for follicular growth, oocytes quality, quantity and developmental competence. Therefore the aim of present study was carried out to investigated whether the seasons (hot and cool) effect on follicular development, oocyte recovery and subsequent embryo development. Follicular oocytes were aspirated from Korean native cows (Hanwoo) by the ovum pick-up (OPU) method, which was performed 24 times during two different seasons, the hot (July to September) and cool (October to December), from OPU donors. The recovered oocytes were classified according to morphological categories and used for in vitro embryo production (IVEP). The mean number of total follicles was significantly higher (p<0.05) during the hot season ($18.32{\pm}2.26$) compared to cool season ($15.41{\pm}3.34$). Furthermore, seasons did not significantly effect on the number of oocytes recovered (hot season: 41.16% vs. cool season: 46.14%). However, the average number of Grade A oocytes was significantly greater during hot ($1.75{\pm}1.86$) season compared to the cool season ($1.00{\pm}1.46$), but there was no significant difference of other grades oocytes. The cleavage rate (hot: 66.67% vs. cool: 63.3%) and embryo development (hot: 58.95% vs. cool: 56.97%) did not differ significantly between the seasons. In conclusion, the results of present study suggest that the season (hot and cool) does not have effects on the oocyte recovery and embryo developmental competence of in vitro cultured embryos.

본 연구에서 한우를 공란우로 사용하여 OPU 방법으로 가장 더운 계절의 hot season과 선선한 cool season의 두 계절의 차이에 따른 생성된 난포의 수, 난자 회수율, 난자 등급율, 수정율 및 배반포 발달 능력을 분석하여, 두 계절이 공란우의 번식 능력에 미치는 영향에 관하여 조사하였다. 1. 계절의 영향이 OPU 공란우의 난포 생성 수에 미치는 결과는 난포 생성 개수는 1154개($18.32{\pm}2.26$), 971개($15.41{\pm}3.34$)로 hot season 그룹이 유의적으로 높은 것을 알 수 있었다(p<0.05). 2. 계절에 따른 난자 수 및 난자 회수율은 hot season 그룹의 475개($7.54{\pm}3.14$), 41.16%로 cool season 그룹 448개($7.11{\pm}3.42$), 46.14%와 비교하여 유의적인 차이가 없었다(p<0.05). 3. OPU를 통하여 회수된 두 계절별 난자 등급은 Grade A는 Hot season 그룹 110개($1.75{\pm}1.86$), Cool season 그룹 63개($1.00{\pm}1.46$)로 hot season 그룹이 cool season 그룹과 비교하여 유의적으로 높았다(p<0.05). 하지만 다른 등급인 Grade B는 87개($1.38{\pm}1.60$) vs. 97개($1.54{\pm}1.39$), Grade C는 166개($2.63{\pm}2.43$) vs. 170개($2.70{\pm}2.04$), Grade D는 112개($1.78{\pm}2.65$) vs. 118개($1.87{\pm}1.86$)로 hot season과 cool season 간의 유의적인 차이를 보이지 않았다(p<0.05). 4. 계절에 따른 체외 수정 후의 수정률은 hot season과 cool season 각각 242(66.67%)와 209(63.3%), 배반포 발달율 214(58.95%) vs. 188(56.97%)로 수정률과 배반포 발달율은 유의적인 차이가 없었다(p<0.05). 본 연구의 결과로 계절에 따른 영향에 의해 공란우의 난포생성수와 A등급의 난자 출현율에서 유의적인 차이를 보였다. 하지만 나머지 등급의 난자 출현율, 수정률 및 배반포 발달율은 차이가 없는 것으로 보아, 계절의 차이로 인한 한우 공란우의 번식 능력에 미치는 영향은 미비하다고 판단된다.

Keywords

References

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