Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of Acarbose Addition on Ruminal Bacterial Microbiota, Lipopolysaccharide Levels and Fermentation Characteristics In vitro

  • Yin, Yu-Yang (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Liu, Yu-Jie (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Zhu, Wei-Yun (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Mao, Sheng-Yong (College of Animal Science and Technology, Nanjing Agricultural University)
  • Received : 2014.04.22
  • Accepted : 2014.08.04
  • Published : 2014.12.01
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Abstract

This study investigated the effects of acarbose addition on changes in ruminal fermentation characteristics and the composition of the ruminal bacterial community in vitro using batch cultures. Rumen fluid was collected from the rumens of three cannulated Holstein cattle fed forage ad libitum that was supplemented with 6 kg of concentrate. The batch cultures consisted of 8 mL of strained rumen fluid in 40 mL of an anaerobic buffer containing 0.49 g of corn grain, 0.21 g of soybean meal, 0.15 g of alfalfa and 0.15g of Leymus chinensis. Acarbose was added to incubation bottles to achieve final concentrations of 0.1, 0.2, and 0.4 mg/mL. After incubation for 24 h, the addition of acarbose linearly decreased (p<0.05) the total gas production and the concentrations of acetate, propionate, butyrate, total volatile fatty acids, lactate and lipopolysaccharide (LPS). It also linearly increased (p<0.05) the ratio of acetate to propionate, the concentrations of isovalerate, valerate and ammonia-nitrogen and the pH value compared with the control. Pyrosequencing of the 16S rRNA gene showed that the addition of acarbose decreased (p<0.05) the proportion of Firmicutes and Proteobacteria and increased (p<0.05) the percentage of Bacteroidetes, Fibrobacteres, and Synergistetes compared with the control. A principal coordinates analysis plot based on unweighted UniFrac values and molecular variance analysis revealed that the structure of the ruminal bacterial communities in the control was different to that of the ruminal microbiota in the acarbose group. In conclusion, acarbose addition can affect the composition of the ruminal microbial community and may be potentially useful for preventing the occurrence of ruminal acidosis and the accumulation of LPS in the rumen.

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References

  1. Baker, G., J. J. Smith, and D. A. Cowan. 2003. Review and reanalysis of domain-specific 16S primers. J. Microbiol. Methods 55:541-555. https://doi.org/10.1016/j.mimet.2003.08.009
  2. Baker, S. B. and W. H. Summerson. 1941. The colorimetric determination of lactic acid in biological material. J. Biol. Chem. 138:535-554.
  3. Blanch, M., S. Calsamiglia, M. Devant, and A. Bach. 2010. Effects of acarbose on ruminal fermentation, blood metabolites and microbial profile involved in ruminal acidosis in lactating cows fed a high-carbohydrate ration. J. Dairy Res. 77:123-128. https://doi.org/10.1017/S0022029909990562
  4. Boone, D. R., R. W. Castenholz, and G. M. Garrity. 2001. Bergey's manual of systematic bacteriology. 2nd Ed. Springer. New York, NY, USA.
  5. Cook, N. B., K. V. Nordlund, and G. R. Oetzel. 2004. Environmental influences on claw horn lesions associated with laminitis and subacute ruminal acidosis in dairy cows. J. Dairy Sci. 87(Supp. l): E36-E46. https://doi.org/10.3168/jds.S0022-0302(04)70059-4
  6. Denman, S. E. and C. S. McSweeney, 2006. Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. FEMS Microbiol. Ecol. 58:572-582. https://doi.org/10.1111/j.1574-6941.2006.00190.x
  7. Desnoyers, M., R. S. Giger-Reverdin, G. Bertin, C. Duvaux-Ponter, and D. Sauvant. 2009. Meta-analysis of the influence of Saccharomyces cerevisiae supplementation on ruminal parameters and milk production of ruminants. J. Dairy Sci. 92:1620-1632. https://doi.org/10.3168/jds.2008-1414
  8. Garrett, E. F., K. V. Nordlund, W. J. Goodger, and G. R. Oetzel. 1997. A cross-sectional field study investigating the effect of periparturient dietary management on ruminal pH in early lactation dairy cows. J. Dairy Sci. 80(Suppl. 1):169 (Abstr).
  9. Good, I. L. 1953. The population frequencies of species and the estimation of population parameters. Biometrika 40(3-4):237-264. https://doi.org/10.1093/biomet/40.3-4.237
  10. Krause, K. M. and G. R. Oetzel. 2006. Understanding and preventing subacute ruminal acidosis in dairy herds. A review. Anim. Feed Sci. Technol. 126:215-236. https://doi.org/10.1016/j.anifeedsci.2005.08.004
  11. Mao, S. Y., G. Zhang, and W. Y. Zhu. 2007. Effect of disodium fumarate on in vitro rumen fermentation of different substrates and rumen bacterial communities as revealed by denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA. Asian Australas. J. Anim. Sci. 20:543-549. https://doi.org/10.5713/ajas.2007.543
  12. Mao, S. Y., R. Y. Zhang, D. S. Wang, and W. Y. Zhu. 2013. Impact of subacute ruminal acidosis (SARA) adaptation on rumen microbiota in dairy cattle using pyrosequencing. Anaerobe 24:12-19. https://doi.org/10.1016/j.anaerobe.2013.08.003
  13. McLaughlin, C. L., A. Thompson, K. Greenwood, J. Sherington, and C. Bruce. 2009a. Effect of acarbose on acute acidosis. J. Dairy Sci. 92:2758-2766. https://doi.org/10.3168/jds.2008-1602
  14. McLaughlin, C. L., A. Thompson, K. Greenwood, J. Sherington, and C. Bruce. 2009b. Effect of acarbose on milk yield and composition in early-lactation dairy cattle fed a ration to induce subacute ruminal acidosis. J. Dairy Sci. 92:4481-4488. https://doi.org/10.3168/jds.2008-1852
  15. Moon, C. D., D. M. Pacheco, W. J. Kelly, S. C. Leahy, D. Li, J. Kopecny, and G. T. Attwood. 2008. Reclassification of Clostridium proteoclasticum as Butyrivibrio proteoclasticus comb. nov., a butyrate-producing ruminal bacterium. Int. J. Syst. Evol. Microbiol. 58:2041-2045. https://doi.org/10.1099/ijs.0.65845-0
  16. Nagaraja, T. G., E. E. Bartley, L. R. Fina, and H. D. Anthony. 1978. Relationship of rumen gram-negative bacteria and free endotoxin to lactic acidosis in cattle. J. Anim. Sci. 47:1329-1336. https://doi.org/10.2527/jas1978.4761329x
  17. Nagaraja, T. G. and E. C. Titgemeyer. 2007. Ruminal acidosis in beef cattle: the current microbiological and nutritional outlook. J. Dairy Sci. 90(Supp. l):E17-E38. https://doi.org/10.3168/jds.2006-478
  18. Packer, E. L., E. H. Clayton, and P. M. V. Cusack. 2011. Rumen fermentation and live weight gain in beef cattle treated with monensin and grazing lush forage. Aust. Vet. J. 89:338-345. https://doi.org/10.1111/j.1751-0813.2011.00802.x
  19. Plaizier, J. C., D. O. Krause, G. N. Gozho, and B. W. McBride. 2008. Subacute ruminal acidosis in dairy cows: The physiological causes, incidence and consequences. Vet. J. 176:21-31. https://doi.org/10.1016/j.tvjl.2007.12.016
  20. Qin, W. L. 1982. Determination of rumen volatile fatty acids by means of gas chromatography. J. NJ Agric. Col. 4:110-116.
  21. Remling, N., S. Riede, P. Lebzien, U. Meyer, M. Holtershinken, S. Kersten, and S. Danicke. 2013. Effects of fumaric acid on rumen fermentation, milk composition and metabolic parameters in lactating cows. J. Anim. Physiol. Anim. Nutr. (In press).
  22. Russell, J. B. and G. F. Diez. 1997. The effects of fermentation acids on bacterial growth. Adv. Microb. Physiol. 39:205-234. https://doi.org/10.1016/S0065-2911(08)60017-X
  23. Speight, S. M., D. L. Harmon, and J. M. Tricarico. 2007. Application of carbohydrase inhibitors to moderate rumen fermentation: continuous culture evaluation. J. Dairy Sci. 90(Suppl. 1):340 (Abstr).
  24. Theodorou, M. K., B. A. Williams, M. S. Dhanoa, A. B. McAllan, and J. France. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim. Feed Sci. Technol. 48:185-197. https://doi.org/10.1016/0377-8401(94)90171-6
  25. Wang, Q., G. M. Garrity, J. M. Tiedje, and J. R. Cole. 2007. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl. Envitron. Microbiol. 73:5261-5267. https://doi.org/10.1128/AEM.00062-07
  26. Weathburn, M. W. 1967. Phenol-Hypochlorite reaction for determination of ammonia. Anal. Chem. 39:971-974. https://doi.org/10.1021/ac60252a045
  27. Zebeli, Q. and B. U. Metzler-Zebeli. 2012. Interplay between rumen digestive disorders and diet-induced in flammation in dairy cattle. Res. Vet. Sci. 93:1099-1108. https://doi.org/10.1016/j.rvsc.2012.02.004

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