Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Distribution and Antimicrobial Resistance of Vibrio parahaemolyticus Isolated from Korean Shellfish

한국 연안산 패류 중 Vibrio parahaemolyticus의 분포 및 항생제내성 특성 비교

  • Yu, Hongsik (Food Safety Division, National Fisheries Research and Development Institute) ;
  • Oh, Eun-Gyoung (West Sea Fisheries Institute, National Fisheries Research and Development Institute) ;
  • Shin, Soon Bum (Southwest Sea Fisheries Institute, National Fisheries Research and Development Institute) ;
  • Park, Yong Su (Food Safety Division, National Fisheries Research and Development Institute) ;
  • Lee, Hee-Jung (Food Microbiology Division, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Ji Hoe (Food Safety Division, National Fisheries Research and Development Institute) ;
  • Song, Ki Cheol (West Sea Fisheries Institute, National Fisheries Research and Development Institute)
  • 유홍식 (국립수산과학원 식품안전과) ;
  • 오은경 (국립수산과학원 서해수산연구소) ;
  • 신순범 (국립수산과학원 남서해수산연구소) ;
  • 박용수 (국립수산과학원 식품안전과) ;
  • 이희정 (식품의약품안전평가원 미생물과) ;
  • 김지회 (국립수산과학원 식품안전과) ;
  • 송기철 (국립수산과학원 서해수산연구소)
  • Received : 2014.08.25
  • Accepted : 2014.09.12
  • Published : 2014.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

The contamination status of Vibrio parahaemolyticus in commercially valuable shellfish from the south and west coasts of Korea and the antimicrobial resistance patterns of isolated V. parahaemolyticus were investigated from July through October, 2011. The range of V. parahaemolyticus concentrations in oysters Crassostrea gigas and short neck clams Venerupis philippinarum was <30~290 MPN/100 g and <30~46,000 MPN/100 g, respectively, and greater than 10,000 MPN/100 g of V. parahaemolyticus was detected from 7 of 40 short neck clams. During the survey period, 436 strains of V. parahaemolyticus were isolated (129 from oysters and 307 from short-neck clams) and the antimicrobial resistance patterns of all of the isolates were examined. Antimicrobial resistance against at least one antibiotic was seen in 79.8% of the oyster isolates (103 strains) and 63.8% of the short neck clam isolates (196 strains). The antimicrobial resistance patterns were relatively simple because the antimicrobial resistance of the isolates was simply due to resistance to ampicillin. Only one oyster isolate and three short neck clam isolates showed multiple antibiotic resistance, i.e., resistance against more than four antibiotics.

Keywords

References

  1. Acar JF and Goldstein FW. 1991. Disk susceptibility test. In: Antibiotics in laboratory medicine. Lorian V, ed. Williams & Wilkins, Baltimore, USA, 17-52.
  2. Angelo D, Jessica LN, John CB, Joy GW, and David WC. 2003. Seasonal Abundance of Total and Pathogenic Vibrio parahaemolyticus in Alabama Oysters. Appl Environ Microbiol 69, 1521-1526. http://dx.doi.org/10.1128/AEM.69.3.1521-1526.2003.
  3. Annie MC and Marta GC. 2012. Vibrio parahaemolyticus in Rhode Island Coastal Ponds and the Estuarine Environment of Narragansett Bay. Appl Environ Microbiol 78, 2996-2999. http://dx.doi.org/10.1128/AEM.07519-11.
  4. Chao G, Jiao X, Zhou X, Yang Z, Huan J, Pan Z, Zhou L and Qian X. 2009. Serodiversity,319 pandemic O3:K6 clone, molecular typing, and antibiotic susceptibility of foodborne and clinical 320 Vibrio parahaemolyticus isolates in Jiangsu, China. Foodborne Pathog Dis 6, 1021-1028. https://doi.org/10.1089/fpd.2009.0295
  5. Das B, Manna SK, Sarkar P and Batabyal K. 2009. Occurrence of Vibrio parahaemolyticus in different finfish and shellfish species. J Food Safety 29, 118-125. http://dx.doi.org/10.1111/j.1745-4565.2008.00146.x.
  6. Diggles BK, Moss GA, Carson J and Anderson CD. 2000. Luminous vibriosis in rock lobster Jasus verreauxi (Decapoda: Palinuridae) phyllosoma larvae associated with infection by Vibrio harvey. Dis Aquat Org 43, 127-137. https://doi.org/10.3354/dao043127
  7. Elliot EL, Kaysner CA, Jackson L and Tamplin ML. 1995. Vibrio cholerae, V. parahaemolyticus, V. vulnificus and other Vibrio spp. In: Bacteriological Analytical Manual, Association of Official Analytical Chemists, Arlington, U.S.A., 9.01-9.27.
  8. Hunter C, Perkins J, Tranter J and Gunn J. 1999. Agricultural land-use effects on the indicator bacterial quality of an upland stream in the Derbyshire Peak District in the UK. Water Res 33, 3577-3586. https://doi.org/10.1016/S0043-1354(99)00083-4
  9. Ju JW. 1983. Studies on Vibrio parahaemolyticus on the southern seas of Korea -On the isolation of V. parahaemolyticus from sea water, sea mud and marine products in Juju, Koeje, Namhae, Yockji, Busan and Masan. J Korea Soc Microbiol 18, 1-9.
  10. KFDA. 2014. Food poisoning statistics by pathogen and month. Retrieved from http://www.mfds.go.kr/e-stat/index.do?nMenuCode=32 on August 23 2014.
  11. Kirs M, DePaola A, Fyfe R, Jones JL, Krantz J, Van Laanen A, Cotton D and Castle M. 2011. A survey of oysters (Crassostrea gigas) in New Zealand for Vibrio parahaemolyticus and Vibrio vulnicus. Int J Food Microbiol 147, 149-153. https://doi.org/10.1016/j.ijfoodmicro.2011.03.012
  12. Kummerer K. 2009. Antibiotics in the aquatic environment: a review - part I. Chemosphere, 417-434. http://dx.doi.org/10.1016/j.ijfoodmicro.2011.03.012.
  13. Lee H, Oh YH, Park SG and Choi SM 2007. Antibiotic susceptibility and distribution of Vibrio parahaemolyticus isolated from the seafood. Kor J Env Hlth 33, 16-20. https://doi.org/10.5668/JEHS.2007.33.1.016
  14. Lee HW, Lee SK and Kim MS. 2008. Characteristics of ampicillin-resistant Vibrio spp. isolated from a west coastal area of Korean Peninsula. J Kor Fish Soc 42, 20-25. https://doi.org/10.5657/kfas.2009.42.1.020
  15. Lipp EK, Farrah SA and Rose JB. 2001. Assessment and impact of microbial fecal pollution and human enteric pathogens in a coastal community. Mar Pollut Bull 42, 286-293. https://doi.org/10.1016/S0025-326X(00)00152-1
  16. Mallin MA, Ensign SH, McIver MR, Shank GC and Fowler PK. 2001. Demographic, landscape, and meteorological factors controlling the microbial pollution of coastal waters. Hydrobiologia 460, 185-193. http://dx.doi.org/10.1023/A:1013169401211.
  17. Metcalf TG. 1978. Indicators of viruses in shellfish. In: Indicators of viruses in water and food. Berg G, ed. Ann Harbor Science, Michigan, 383-415.
  18. Miossec L, Guyader F, Haugarreau L, Comps MA and Pommepuy M. 1988. Possible relation between a winter epidemic of acute gastroenteritis in France and viral contamination of shellfish. J Shellfish Res 17, 1661-1664.
  19. MOF. 2014. Fisheries production statistics. Retrieved from http://www.fips.go.kr/jsp/sf/ss/ss_info.jsp?menuDepth=070101 on August 23 2014.
  20. Morris JG and Tenny J Jr. 1985. Antibiotic therapy for Vibrio vulnificus infection. J Am Med Assoc 253, 1121-1122. https://doi.org/10.1001/jama.1985.03350320041011
  21. Neela FA, Nonaka L and Suzuki S. 2007. The diversity of multidrug resistance profiles in tetracycline-resistant Vibrio species isolated from coastal sediments and seawater. J Microbiol 45, 64-68.
  22. Oh EG, Son KT, Ha KS, Yoo HD, Yu HS, Shin SB, Lee HJ and Kim JH. 2009. Antimicrobial Resistance of Vibrio strains from Brackish Water on the Coast of Gyeongsangnamdo. J Kor Fish Soc 42, 335-343. https://doi.org/10.5657/kfas.2009.42.4.335
  23. Oh EG, Son KT, Yu HS, Lee TS, Lee HJ, Shin SB, Kwon JY, Park KBW and KIM JH. 2011. Antimicrobial resistance of Vibrio parahaemolyticus and Vibrio alginolyticus strains isolated from Farmed Fish in Korea during 2005-2007. J Food Protec 74, 380-386. https://doi.org/10.4315/0362-028X.JFP-10-307
  24. Ortigosa M, Garay E and Pujalte MJ. 1994. Numerical taxonomy of vibrionaceae isolated from oysters and seawater along an annual cycle. Syst Appl Microbiol 17, 216-225. https://doi.org/10.1016/S0723-2020(11)80011-1
  25. Parveen S, Hettiarachchi KA, Bowers JC, Jones JL, Tamplin ML, McKay R, Beatty W, Brohawn K, Dasilva LV, Depaola A. 2008. Seasonal distribution of total and pathogenic Vibrio parahaemolyticus in Chesapeake Bay oysters and waters. Int J Food Microbiol 128, 354-361. http://dx.doi.org/10.1016/j.ijfoodmicro.2008.09.019.
  26. Rhodes BJ, Smith HL Jr. and Ogg JE. 1986. Isolation of non-O1 Vibrio cholerae serovars from surface waters in Western Colorado. Appl Environ Microbiol, 51, 1216-1219.
  27. Sobsey MD and Jaykus LA. 1991. Human enteric viruses and depuration of bivalve molluscs. In: Molluscan shellfish depravation. Otwell WS, Rodrick GE and Martin RE, eds. CRC Press, Boca Raton, Florida, USA, 71-114.
  28. Son KT, Oh EG, Lee TS, Lee HJ, Kim PH and Kim JH. 2005. Antimicrobial susceptibility of Vibrio parahaemomlyticus and Vibrio alginolyticus from fish farms on the southern coast of Korea. J Kor Fish Soc 38, 365-371.
  29. Urakawa H, Yoshida T, Nishimura M and Ohwada K. 2000. Characterization of depth related population variation in microbial communities of coastal marine sediment using 16SrDNA-based approaches and quinone profiling. Environ Microbiol 2, 542-554. https://doi.org/10.1046/j.1462-2920.2000.00137.x
  30. Zanetti S, Spanu T, Deriu A, Romano L, Sechi LA and Fadda G. 2001. In vitro susceptibility of Vibrio spp. isolated from the environment. Int J Antimicrob Agents 17, 407-409. https://doi.org/10.1016/S0924-8579(01)00307-7
  31. Zimmerman AM, DePaola A, Bowers JC, Krantz JA, Nordstrom JL, Johnson CN and Grimes DJ. 2007. Variability of total and pathogenic Vibrio parahaemolyticus densities in northern Gulf of Mexico water and oysters. Appl Environ Microbiol 73, 7589-7596. https://doi.org/10.1128/AEM.01700-07

Cited by

  1. Virulence Factors and Antimicrobial Susceptibility of Vibrio parahaemolyticus Isolated from the Oyster Crassostrea gigas vol.49, pp.2, 2016, https://doi.org/10.5657/KFAS.2016.0116