Journal of Korean Society of Environmental Engineers (대한환경공학회지)

Korean Society of Environmental Engineers (대한환경공학회)
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Variations of Hydrogen Production and Microbial Community with Different Nitrogen Concentration During Food Waste Fermentation

음식물쓰레기의 혐기성 소화 시 질소농도에 따른 수소생산 및 미생물 군집변화

  • Lee, Pul-Eip (Department of Environmental Engineering, Seoul National University of Science & Technology) ;
  • Lee, Tae-Jin (Department of Environmental Engineering, Seoul National University of Science & Technology)
  • 이풀잎 (서울과학기술대학교 환경공학과) ;
  • 이태진 (서울과학기술대학교 환경공학과)
  • Received : 2014.07.02
  • Accepted : 2014.10.16
  • Published : 2014.10.30
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Abstract

In this study, variations of fermentative hydrogen production and microbial community were investigated with different nitrogen concentration of food waste. Optimum hydrogen production rate was acquired at 200 mg/L nitrogen concentration of the food waste. Which was eqivalent to 83.43 mL/g dry biomass/hr. However, bio-hydrogen production was inhibitedly reduced at over 600 mg/L of nitrogen concentration whereas proportional relation between hydrogen production and B/A ratio were not observed. Most dominant specie of the microbial community analyzed was Clostridium sp. throughout PCR-DGGE analysis of 16S rDNA. It revealed that most contributing microorganism producing hydrogen were Enterococcus faecium partial, Klebsiella pneumoniae strain ND6, Enterobacter sp. NCCP-231, and Clostridium algidicarnis strain E107 in this experiment.

본 연구는 음식물 쓰레기 내 질소농도에 따른 발효과정에서 수소생성 특성과 미생물의 군집변화를 살펴보았다. 음식물 쓰레기 내 질소의 함량이 200 mg/L일 때 가장 높은 수소생산 효율을 보여주었으며, 이 때 수소생산율은 83.43 mL/g dry wt biomass/hr이였다. 질소의 함량이 600 mg/L 이상이 되면 수소생산이 저해되는 것으로 나타났으며 수소생산량과 B/A ratio (Butric acid/Acetic acid)의 비례적 상관관계는 관찰되지 않았다. 16S rDNA의 PCR-DGGE결과 대부분 군집은 Clostridium sp. 미생물로 규명되었으며 수소생성에 기여도가 큰 미생물은 Enterococcus faecium partial, Klebsiella pneumoniae strain ND6, Enterobacter sp. NCCP-231, 그리고 Clostridium algidicarnis strain E107 등으로 판명되었다.

Keywords

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