Journal of Plant Biotechnology

  • 제41권3호
  • /
  • Pages.127-133
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  • 2014
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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벼물바구미 (Lissorhoptrus oryzophilus) 내충성 GM 벼에서 T-DNA와 게놈의 인접부위 분석

Analysis of junction site between T-DNA and plant genome in Lissorhoptrus oryzophilus resistance GM rice

  • 이진형 (국립농업과학원 농업생명자원부) ;
  • 신공식 (국립농업과학원 농업생명자원부) ;
  • 서석철 (국립농업과학원 농업생명자원부) ;
  • 임성렬 ((주)인우코퍼레이션) ;
  • 임명호 (국립농업과학원 농업생명자원부) ;
  • 우희종 (국립농업과학원 농업생명자원부) ;
  • 친양 (국립농업과학원 농업생명자원부) ;
  • 권순종 (국립농업과학원 농업생명자원부) ;
  • 박순기 (국립농업과학원 농업생명자원부)
  • Lee, Jin-Hyoung (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Shin, Kong-Sik (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Suh, Seok-Cheol (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Rhim, Seong-Lyul (Inwoo Cooperation Ltd.) ;
  • Lim, Myung-Ho (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Woo, Hee-Jong (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Qin, Yang (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kweon, Soon-Jong (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Soon-Ki (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
  • 투고 : 2014.07.15
  • 심사 : 2014.09.25
  • 발행 : 2014.09.30
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초록

Bacillus thuringiensis 균주로부터 분리한 cry3A 유전자는 딱정벌레목 해충에 살충성을 가지고 있어서 효율적으로 딱정벌레목 해충을 방제 할 수 있다고 알려져 있다. 국립농업과학원에서는 cry3A 유전자 형질전환벼를 육성하였고, GMO 포장 생물검정 연구를 통해 벼물바구미에 대한 내충성을 확인하였다. 본 연구에서는 벼물바구미 내충성 벼 4 계통에 대한 도입유전자 특성을 분자생물학적 방법으로 검정하여 유전자의 도입 사본 수와 도입유전자의 염기서열 등의 분석결과를 제시하였다. BT12R1 계통은 염색체 10번의 exon 부위에 도입유전자가 단일 사본으로 삽입되었으며, BT12R2 계통은 두 개의 사본으로 삽입되었고, BT12R3 계통은 LB 인근의 염기서열만 확인되었다. BT12R4 계통의 경우 단일 사본의 도입유전자가 염색체 5번의 24,516,607 ~ 24,516,636 위치에 30개의 염색체 염기서열이 결실된 상태로 삽입되었다. 이 도입위치는 벼의 내재 유전자가 발현하지 않는 intergenic 부위로 판명되었으며, 도입유전자 이외의 벡터 염기서열이 삽입되지 않음을 확인하였다. 이러한 벼물바구미 내충성벼 BT12R4 계통에 대한 분자생물학적 분석 결과는 차후 GM 작물 실용화의 환경 위해성 및 안전성 평가에 대한 기초자료로 활용할 수 있을 것으로 생각된다.

Four transgenic rice lines harboring insect-resistant gene cry3A showed ideal field performances characterized by high considerable resistance to rice water weevil (Lissorhoptrus oryzophilus Kuschel). In this study, we estimated the insertion number of foreign genes, and analyzed the flanking sequences of T-DNAs in rice genome. As a result, T-DNA of BT12R1 line was inserted in exon region of rice chromosome 10. Two copies of T-DNAs were inserted in line BT12R2. BT12R3 line was analyzed at only left border flanking sequence. BT12R4 line was confirmed one copy of foreign gene insertion at the position 24,516,607 ~ 24,516,636 of rice chromosome 5, accompanied by a deletion of 30 bp known genomic sequences. This intergenic position was confirmed none of expressed gene and any deletion/addition of T-DNA sequence. In conclusion, these molecular data of rice water weevil resistant Bt rice would be used to conduct the biosafety and environment risk assessment for GM crop commercialization.

키워드

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