Biomaterials Research (생체재료학회지)

Korean Society for Biomaterials (한국생체재료학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of osteoconductive hyaluronate hydrogels on calvarial bone regeneration

  • Yeom, Junseok (Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH)) ;
  • Hwang, Byung Woo (Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH)) ;
  • Yang, Dong Jun (MegaGen Research Institute of Science and Technology) ;
  • Shin, Hong-In (Department of Oral Pathology, School of Dentistry, IHBR, Kyungpook National University) ;
  • Hahn, Sei Kwang (Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH))
  • Received : 2014.06.12
  • Accepted : 2014.07.09
  • Published : 2014.09.01
⟨ Previous Next ⟩

Abstract

Background: Without exploitation of possibly immunogenic and carcinogenic bone morphogenetic protein, we developed simple but clinically feasible artificial bone graft using osteoconductive hyaluronate (HA) hydrogels and bioactive MegaGen synthetic bone (MGSB). Methods: HA hydrogels were synthesized by the crosslinking reaction between carboxyl groups of HA and amine groups of gelatin (GEL). Then, artificial bone grafts were prepared by mixing MGSB with HA-GEL hydrogels. The bone regeneration by the MGSB/HA-GEL hydrogel complex was assessed in the skull of New Zealand white male rabbits in 4 and 8 weeks. Results: HA hydrogels were synthesized by the crosslinking reaction between carboxyl groups of HA and amine groups of gelatin (GEL). Then, artificial bone grafts were prepared by mixing MGSB with HA-GEL hydrogels. In vitro proliferation of preosteogenic cells was enhanced with increasing molecular weight of HA. In addition, histological analysis of dissected tissues with hematoxylin and eosin staining confirmed the effective in vivo bone regeneration by the MGSB/HA-GEL hydrogel complex. The MGSB/HA-GEL hydrogels were well resorbed and partially substituted to the lamellar bone after implantation for 8 weeks. Conclusions: The novel artificial bone graft of MGSB/HA-GEL hydrogel complex for effective bone regeneration might be clinically feasible for further development.

Keywords

Acknowledgement

Supported by : National Research Foundation (NRF)

References

  1. Ducy P, Schinke T, Karsenty G: The osteoblast: a sophisticated fibroblast under central surveillance. Science 2000, 289:1501-1504. https://doi.org/10.1126/science.289.5484.1501
  2. Ratner BD, Hoffman AS, Schoen FJ: Biomaterials science. 3rd edition. The Boulevard, Langford Lane, Kidlington: Academic Press; 2013.
  3. Duer M, Veis A: Bone mineralization: water brings order. Nat Mater 2013, 12:1081-1082. https://doi.org/10.1038/nmat3822
  4. Teitelbaum SL: Bone resorption by osteoclasts. Science 2000, 289:1504-1508. https://doi.org/10.1126/science.289.5484.1504
  5. Deschaseaux F, Sensebe L, Heymann D: Mechanisms of bone repair and regeneration. Trends Mol Med 2009, 15:417-429. https://doi.org/10.1016/j.molmed.2009.07.002
  6. Doblare M, Garcia JM, Gomez MJ: Modelling bone tissue fracture and healing: a review. Eng Fract Mech 2004, 71:1809-1840. https://doi.org/10.1016/j.engfracmech.2003.08.003
  7. Bose S, Roy M, Bandyopadhyay A: Recent advances in bone tissue engineering scaffolds. Trends Biotechnol 2012, 30:546-554. https://doi.org/10.1016/j.tibtech.2012.07.005
  8. Seliktar D: Designing cell-compatible hydrogels for biomedical applications. Science 2012, 336:1124-1128. https://doi.org/10.1126/science.1214804
  9. Merkx MA, Maltha JC, Kuijpers-Jagtman AM: Incorporation of three types of bone block implants in the facial skeleton. Biomaterials 1999, 20:639-645. https://doi.org/10.1016/S0142-9612(98)00219-1
  10. Jung RE, Weber FE, Hammerle CH: Bone morphogenetic protein-2 enhances bone formation when delivered by a synthetic matrix containing hydroxyapatite / tricalciumphosphate. Clin Oral Implants Res 2008, 19:188-195. https://doi.org/10.1111/j.1600-0501.2007.01431.x
  11. Kim J, Kim IS, Sun K: Bone regeneration using hyaluronic acid-based hydrogel with bone morphogenic protein-2 and human mesenchymal stem cells. Biomaterials 2007, 28:1830-1837. https://doi.org/10.1016/j.biomaterials.2006.11.050
  12. Laurent TC, Laurent UB, Fraser JR: The structure and function of hyaluronan: an overview. Immunol Cell Biol 1996, 74:A1-A7. https://doi.org/10.1038/icb.1996.32
  13. Maus U, Andereya S, Niedhart C: Lack of effect on bone healing of injectable BMP-2 augmented hyaluronic acid. Arch Orthop Trauma Surg 2008, 128:1461-1466. https://doi.org/10.1007/s00402-008-0608-8
  14. Suzuki K, Anada T, Suzuki O: Effect of addition of hyaluronic acids on the osteoconductivity and biodegradability of synthetic octacalcium phosphate. Acta Biomater 2014, 10:531-543. https://doi.org/10.1016/j.actbio.2013.09.005
  15. Chao KL, Muthukumar L, Herzberg O: Structure of human hyaluronidase-1, a hyaluronan hydrolyzing enzyme involved in tumor growth and angiogenesis. Biochemistry 2007, 46:6911-6920. https://doi.org/10.1021/bi700382g
  16. Slevin M, Kumar S, Gaffney J: Angiogenic oligosaccharides of hyaluronan induce multiple signaling pathways affecting vascular endothelial cell mitogenic and wound healing responses. J Biol Chem 2002, 277:41046-41059. https://doi.org/10.1074/jbc.M109443200
  17. Kim HW, Song JH, Kim HE: Nanofiber generation of gelatin-hydroxyapatite biomimetics for guided tissue regeneration. Adv Funct Mater 2005, 15:1988-1994. https://doi.org/10.1002/adfm.200500116
  18. Yeom J, Chang S, Hahn SK: Synchrotron X-ray bioimaging of bone regeneration by artificial bone substitute of megagen synthetic bone and hyaluronate hydrogels. Tissue Eng Part C Methods 2010, 16:1059-1068. https://doi.org/10.1089/ten.tec.2009.0759
  19. Maus U, Andereya S, Niedhart C: Lack of effect on bone healing of injectable BMP-2 augmented hyaluronic acid. Arch Orthop Trauma Surg 2008, 128:1461-1466. https://doi.org/10.1007/s00402-008-0608-8
  20. Shu XZ, Liu Y, Prestwich GD: Disulfide-crosslinked hyaluronan-gelatin hydrogel films: a covalent mimic of the extracellular matrix for in vitro cell growth. Biomaterials 2003, 24:3825-3834. https://doi.org/10.1016/S0142-9612(03)00267-9
  21. Virolainen P, Heikkila J, Aro HT: Histomorphometric and molecular biologic comparison of bioactive glass granules and autogenous bone grafts in augmentation of bone defect healing. J Biomed Mater Res 1997, 35:9-17. https://doi.org/10.1002/(SICI)1097-4636(199704)35:1<9::AID-JBM2>3.0.CO;2-S
  22. Manson PN, Crawley WA, Hoopes JE: Frontal cranioplasty: risk factors and choice of cranial vault reconstructive material. Plast Reconstr Surg 1986, 77:888-904. https://doi.org/10.1097/00006534-198606000-00003
  23. Tabata Y, Ikada Y: Protein release from gelatin matrices. Adv Drug Delivery Rev 1998, 31:287-301. https://doi.org/10.1016/S0169-409X(97)00125-7

Cited by

  1. Preparation and investigation of hydrolyzed polyacrylonitrile as a preliminary biomedical hydrogel vol.19, pp.None, 2014, https://doi.org/10.1186/s40824-015-0043-1
  2. The Islet Confidential: Recent Trends and Perspectives in Pancreatic Islet Transplantation vol.3, pp.None, 2014, https://doi.org/10.18052/www.scipress.com/ijppe.3.54
  3. New Frontiers in Biomaterials Research for Tissue Repair and Regeneration vol.2, pp.2, 2014, https://doi.org/10.18679/cn11-6030_r.2016.017
  4. Effect of hyaluronic acid on morphological changes to dentin surfaces and subsequent effect on periodontal ligament cell survival, attachment, and spreading vol.21, pp.4, 2014, https://doi.org/10.1007/s00784-016-1856-6
  5. Bioimaging of botulinum toxin and hyaluronate hydrogels using zwitterionic near-infrared fluorophores vol.21, pp.1, 2014, https://doi.org/10.1186/s40824-017-0102-x
  6. Evaluation of the Effectiveness of Esterified Hyaluronic Acid Fibers on Bone Regeneration in Rat Calvarial Defects vol.2018, pp.None, 2018, https://doi.org/10.1155/2018/3874131
  7. Immunohaematological and rheological parameters in canine visceral leishmaniasis vol.27, pp.2, 2018, https://doi.org/10.1590/s1984-296120180021
  8. 3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model vol.11, pp.1, 2021, https://doi.org/10.1038/s41598-021-84123-x
  9. Hyaluronate/black phosphorus complexes as a copper chelating agent for Wilson disease treatment vol.25, pp.1, 2014, https://doi.org/10.1186/s40824-021-00221-x