한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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급성 알코올 간독성을 유발한 생쥐에 있어서 으름 열매 추출물의 간 기능 보호효과

Protective Effects of Akebia quinata Fruit Extract on Acute Alcohol-induced Hepatotoxicity in Mice

  • 이상훈 (이화여자대학교 건강과학대학 식품영양학과) ;
  • 송영선 (이화여자대학교 건강과학대학 식품영양학과) ;
  • 이서연 (이화여자대학교 건강과학대학 식품영양학과) ;
  • 김소영 (이화여자대학교 건강과학대학 식품영양학과) ;
  • 고광석 (이화여자대학교 건강과학대학 식품영양학과)
  • Lee, Sang Hoon (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University) ;
  • Song, Young Sun (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University) ;
  • Lee, Seo Yeon (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University) ;
  • Kim, So Young (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University) ;
  • Ko, Kwang Suk (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University)
  • 투고 : 2014.04.07
  • 심사 : 2014.08.29
  • 발행 : 2014.10.31
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초록

생쥐에서의 으름의 열매 추출물의 경구 투여가 알코올로 인한 급성 간독성 상태에서 간보호 효과에 대한 영향을 조사 하였다. 즉, 생쥐 (C57BL/6)에게 1주간 으름 열매 추출물을 투여 하고 희생 전 알코올의 경구 투여를 통해 급성 알코올 간독성을 유발한 후 간 조직 형상, 간 기능 지표(ALT, AST), 간 세포내 GSH 합성 효소(GCLM, GCLC, GSS)의 mRNA 발현량, GSH 농도의 측정, 산화 스트레스 지표인 NOX4의 mRNA 발현량과 염증 반응 지표인 TNF-${\alpha}$의 mRNA 발현량을 조사 하였다. 그 결과, 으름 열매 추출물의 경구 투여는 알코올로 유발된 급성 간독성 상태에서 간 조직내 지방의 축적을 완화 하였고, 혈청 AST, ALT 수치를 개선하였으며, 간조직 내 항산화 물질인 GSH의 농도를 증가시켰다. 더불어 활성산소기를 생성하는 NOX4의 mRNA 발현을 억제 하는 것으로 분석되었으며 염증 반응 지표인 TNF-의 mRNA 발현도 억제 하는 것으로 분석되었다. 따라서 으름의 열매 추출물은 알코올로 유발된 산화 스트레스, 염증 반응에 대한 간보호 효과 가능성을 나타내는 것으로 판단된다.

We studied the effects of Akebia quinata fruit extract (AQ) on acute alcohol-induced hepatotoxicity in mice. AQ (30-1,000 mg/kg body weight (BW) per day) was orally administered to the study group, once daily for 1 week. On the last day of AQ treatment, ethanol (6 mg/kg BW) was orally administered to induce acute liver injury. The AQ-treated group showed significantly lower levels of alanine aminotransferase and aspartate aminotransferase, compared to the only ethanol-treated group (ETG). The glutathione level in the AQ-treated group elevated up to 20.6%, compared to that observed in the ETG. The mRNA expression of glutathione synthetic enzymes was also higher in the AQ-treated group, compared to the ETG. The AQ-treated group also exhibited lower levels of expression of NADPH oxidase 4 and tumor necrosis factor alpha mRNA. Thus, these results show that AQ treatment can be a potential method to reduce oxidative stress and inflammation in ethanol-treated mouse liver and also that AQ can be a useful therapeutic agent for acute alcohol-induced hepatotoxicity.

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