DOI QR코드

DOI QR Code

HaCaT 세포에서 자외선 B에 의해 유도되는 DNA 상해에 대한 국내 수종 자생 식물 에탄올 추출물의 저해효과

Inhibitory Effects of Ethanol Extracts of Some Korean Plants on Ultraviolet B-Induced DNA Damage

  • 이석희 (원광대학교 생명과학부 기초자연과학연구소) ;
  • 하세은 (원광대학교 생명과학부 기초자연과학연구소) ;
  • 조형권 (한풍제약) ;
  • 박종군 (원광대학교 생명과학부 기초자연과학연구소)
  • Lee, Seok Hee (Research Institute for Basic Science and Division of Biological Science, Wonkwang University) ;
  • Ha, Se Eun (Research Institute for Basic Science and Division of Biological Science, Wonkwang University) ;
  • Cho, Hyoung Kwon (Hanpoong Pharm & Foods Co., Ltd.) ;
  • Park, Jong Kun (Research Institute for Basic Science and Division of Biological Science, Wonkwang University)
  • 투고 : 2014.07.30
  • 심사 : 2014.09.30
  • 발행 : 2014.10.31

초록

In the present study, the effects of extracts from Korean plants on the DNA damage response in HaCaT cells exposed to ultraviolet B (UVB) were investigated. The activity of cells treated for 24 hr with ethanol extracts from Vaccinium spp. (VS), and Vitis vinifera L (VV) alone was similar to that of the non-treated control, but gradually decreased at concentrations above $200{\mu}g/mL$. However, when post-incubation of UVB-exposed cells was carried out for 24 hr in medium containing VS or VV extracts, the cell activity increased in a concentration-dependent manner compared with that in the normal growth medium. The cell viability of UVB-exposed cells also increased when post-incubated in medium containing VS or VV extracts, in a concentration-dependent manner. Nuclear fragmentation analysis showed that post-incubation with VS or VV extracts decreased the UVB-induced apoptosis by about 10 and 13%, respectively, of that in cells post-incubated in growth medium. After 24 hr of post-incubation in medium containing VS or VV extracts, the level of CPD and 8-OHdG decreased in time- and concentration-dependent manners. Overall these results suggest that VS and VV extracts assist the survival of UVB-exposed cells, in accordance with the respective decrease in the levels of UVB-induced DNA damage.

키워드

참고문헌

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