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산초 종자 정유의 항산화 및 항염 효능

Anti-oxidant and Anti-inflammatory activities of Zanthoxylum schinifolium Essential Oil

  • 김보애 (목원대학교 테크노과학대학 생의약화장품학부)
  • Kim, Bo-Ae (College of Sciences & Technology, Division of Biomedicinal & Cosmetics, Mokwon University)
  • 투고 : 2014.08.11
  • 심사 : 2014.09.22
  • 발행 : 2014.09.30

초록

본 연구의 목적은 RAW 264.7 세포에 산초 종자 정유 성분의 항염 및 항산화 효과에 대하여 알아보고자 하였다. 산초 종자로부터 정유를 추출하였으며, 추출물의 항산화 및 항염 활성에 대하여 평가하였다. 추출물의 항산화 활성은 DPPH 라디칼 소거능과 SOD 유사 활성 평가법을 이용하였다. 또한 세포독성 평가를 위해 MTT를 이용하여 세포생존율을 측정하였다. LPS로 염증이 유도된 RAW 264.7 세포에 대하여 산초 정유가 NO생성과 $PGE_2$ 생성 정도에 미치는 영향을 측정하였다. 그 결과 산초 종자 정유는 농도 의존적으로 DPPH 라디칼 소거능과 SOD 유사 활성이 증가하는 것으로 나타났다. 세포생존율 평가에서는 $40{\mu}g/mL^{-1}$ 이하의 농도에서 98% 이상의 낮은 세포독성을 나타냈으며, 항염 효능 평가에서는 LPS를 단독으로 처리한 양성대조군보다 산초 정유 성분을 처리한 군에서 NO와 $PGE_2$ 생성이 현저하게 감소되는 것을 확인하였다. 이러한 결과는 산초 정유가 염증매개인자를 감소시키고 산화의 활성을 방지할 수 있는 기능성 식물소재로서의 가능성을 나타낸다.

The purpose of this study was to investigate anti-inflammatory and antioxidant effects of essential oil from seed of Zanthoxylum schinifolium on cultured RAW 264.7 cell line. Antioxidant activity of essential oil was evaluated by two different assays as 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and super oxide dismutase (SOD) like activities. This essential oil was tested for cell viability on RAW 264.7 cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The effects of anti-inflammatory on LPS-induced RAW 264.7 cell line was studied by the content of nitric oxide (NO) and prostagladin $E_2$ ($PGE_2$) in cells. The essential oil of seed from Zanthoxylum schinifolium obtained dose-dependently increased the scavenging activity on DPPH radical scavenging activity and SOD like activity. The essential oil showed low cytotoxicity as more than 98% cell viability in $40{\mu}g/mL^{-1}$ concentration. The essential oil of seed extracted from Zanthoxylum schinifolium presented obvious effect on inflammation. These results suggest that essential oil of seed from Zanthoxylum schinifolium may have value as the potential anti-inflammatory effects by decreasing the action of NO and $PGE_2$ and preventing the activation of oxidative.

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참고문헌

  1. H. K. Seong, Y. H. Lee and C. K. Seong, Herbal home remedies. Happy Blue. Seoul, Korea, 69 (2012).
  2. J. W. Lee, Volatile flavor components of Korean sancho fruit and tree (Zanthoxylum schinifolium). Korean J. Community Nutr., 11, 493 (1998).
  3. S. J. Lee, Korean folk medicinemonographs series. No. 3. Publishing center of Seoul National University, Seoul, Korea, 88 (1996).
  4. M. S. Jung, Y. M. Shin, M. K. Kim, C. H. Kim and J. S. Choi, Physicochemical properties of Sancho (Zanthoxylum schinifolium) seeds oil base extracts from different method. Korean J. Food Preserv., 20(6), 827 (2013). https://doi.org/10.11002/kjfp.2013.20.6.827
  5. T. J. Kim, Korean resources plants. Publishing center of Seoul National University, Seoul, Korea, 266 (1998)
  6. S. M. Bae, Y. M. Jin, E. H. Jeong, M. B. Kim, H. Y. Shin, C. W. Ro and S. C. Lee, Studies on proximate composition, fatty acids and volatile compounds of Zanthoxylum schinifolium fruit according to harvesting time. Korean J. Medicinal Crop Sci., 19(1), 1 (2011). https://doi.org/10.7783/KJMCS.2011.19.1.001
  7. S. Kim, Y. Han, Isolation and identification of antimicrobial compound from Sancho (Zanthoxylum schinifolium). Korean J. Soc Food Sci., 13(1), 56 (1997).
  8. S. K. Hur, S. S. Kim, Y. H. Heo, S. M. Ahn, B. G. Lee and S. K. Lee, Effect of the grapevine shoot extract on free radical scavenging activity and inhibition of pro-inflammatory mediator production in raw 264.7 macrophages. J. Applied Pharmacology. 9(3) : 188 (2001).
  9. M. Roberforid, P. B. Calderon, Free radicals and oxidation phenomena in biological system. Marcel Dekker, Inc., New York. 193 (1995).
  10. J. W. Lee, Volatile flavor components of Korean sancho fruit and tree (Zanthoxylum schinifolium). Korean J. Community Nutr., 11(5), 493 (1998).
  11. Akira Murakami and Hajime Ohigashi, Targeting NOX, iNOS and COX-2 in inflammatory cells. Int. J. Cancer, 121(11), 2357 (2007). https://doi.org/10.1002/ijc.23161
  12. W. K. Alderton, C. E. Cooper and R. G. Knowles, Nitric oxide synthase : Structure, function and inhibition. Biochem J., 357, 593 (2001). https://doi.org/10.1042/0264-6021:3570593
  13. H. S. Ryu, K. H. Chang, H. W. Yang, M. S. Kim, H. C. Kwon and K. S. Oh, High cyclooxygenase-2 expression in stage IB cervical cancer with lymph node metastasis or parametrial invasion. Korean J. Gynecol. Oncol., 76, 320 (2000). https://doi.org/10.1006/gyno.1999.5690
  14. D. K. Gaffney, J. Holden, M. Davis, K. Zempolich, K. J. Murphy and M. Dodson, Elevated cyclooxygenase-2 expression correlates with diminished survival in carcinoma of the cervix treated with radiotherapy. Int J. Radiat Oncol. Biol. Phys., 42(9), 494 (2001).
  15. J. R. Vane, Y. S. Bakhle and R. M. Botting, Cyclooxygenases 1 and 2. Annu. Rev. Pharmacol. Toxicol., 38(1), 97 (1998). https://doi.org/10.1146/annurev.pharmtox.38.1.97
  16. J. Hyuk Lee, K. M. Chang and G. H. Kim, Composition and anti-inflammatory activities of Zanthoxylum schinifolium essential oil:suppression of inducible nitric oxide synthase, cyclooxygenase-2, cytokines and cellular adhesion. J. Science of Food and Agriculture, 89(10), 1762 (2009). https://doi.org/10.1002/jsfa.3653

피인용 문헌

  1. 세토카 가지 정유의 성분 분석 및 생리 활성 vol.42, pp.3, 2016, https://doi.org/10.15230/scsk.2016.42.3.227
  2. 개망초꽃 에센셜 오일의 항산화, 항균 및 항염 활성 vol.33, pp.4, 2014, https://doi.org/10.12925/jkocs.2016.33.4.717
  3. Comparative Study of Bioactivity and Safety Evaluation of Ethanolic Extracts of Zanthoxylum schinifolium Fruit and Pericarp vol.26, pp.19, 2014, https://doi.org/10.3390/molecules26195919