Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Effect of Acetic and Lactic Acid Mixtures on Control of Quarantine Nematode, Bursaphelenchus xylophilus, in Exporting Cymbidium

초산과 젖산 혼합액에 의한 수출용 심비디움 검역선충 Bursaphelenchus xylophilus의 방제 효과

  • Seo, Yunhee (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Park, Jiyeong (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Cho, Myoung Rae (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Chun, Jae Yong (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency) ;
  • Kim, Young Ho (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
  • 서윤희 (서울대학교 농업생명과학대학 농생명공학부, 농생명과학원) ;
  • 박지영 (서울대학교 농업생명과학대학 농생명공학부, 농생명과학원) ;
  • 조명래 (국립원예특작과학원) ;
  • 전재용 (식물검역기술개발센터) ;
  • 김영호 (서울대학교 농업생명과학대학 농생명공학부, 농생명과학원)
  • Received : 2014.07.15
  • Accepted : 2014.09.17
  • Published : 2014.09.30
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Abstract

The mixture (MX) of acetic acid (AA) and lactic acid (LA) was examined for its effectiveness in the control of the pine wood nematode Bursaphelenchus xylophilus contaminated in cymbidium culture medium. Nematode mortality in vitro was nearly 100% in AA and MX at the concentrations of 5.0-1.0% (pH 2.6 - 4.2) and in LA only at 5.0% (pH 3.5), but lowered at concentrations of 0.5-0.1% (pH 5.1-6.9) more significantly in LA than AA and MX. MX of most concentrations caused higher nematode mortality than the average response to AA and LA. All treatments of MX (0.5% and 0.25%), fosthiazate (standard and double concentrations) and culture dilution of Paenibacillus polymyxa GBR-1 ($10^7$colony-forming units/ml) reduced significantly the nematode populations in the cymbidium culture medium, compared to non-treatment control, with no significant difference among the treatments. No phytotoxicity occurred in all treatments. pH of the medium with the time after treatment and growths of 2-year-old cymbidium were not significantly different among treatments. Considering the safety and price of the organic acids, use of MX in the processes for culturing cymbidium may be a practically reliable and eco-friendly way in the control of the quarantine nematode in cymbidium.

초산(acetic acid)과 젖산(lactic acid)의 혼합액(MX)의 심비디움 배양토에 오염된 소나무재선충(Bursaphelenchus xylophilus)의 방제효과를 조사하였다. 실내실험에서 초산과 혼합액은 5.0-1.0% (pH 2.6-4.2)에서 젖산은 5.0%에서만 100%가까운 살선충율을 나타내었고 이보다 낮은 농도(0.5-0.1%, pH 5.1-6.9)에서는 초산과 혼합액보다 젖산에서 살선충력이 유의적으로 낮아졌다. 대부분의 농도에서 혼합액의 살선충율이 초산과 젖산의 평균보다 유의적으로 높게 나타나 혼합액의 살선충력 상승효과가 있을 것으로 판단된다. 폿트실험에서 유기산 혼합액(0.5%, 0.25%), 살선충제(fosthiazate, 표준량, 배량) 및 길항세균(Paenibacillus polymyxa GBR-1) 배양 희석액($10^7CFU/ml$) 등의 처리에 의해 심비디움 배양토에서의 소나무재선충의 밀도가 처리간에는 유의적인 차이가 없이 무처리에 비해 유의적으로 감소하였다. 모든 처리에서 약해가 나타나지 않았고, 처리 후 시기별 pH와 2년생 심비디움의 생육에 무처리 대조구와 유의적인 차이가 나타나지 않았다. 이러한 결과와 이들 식용 유기산의 안전성과 가격을 종합하여 볼 때, 심비디움의 재배 관리 중 생육단계에 따라 이 유기산 혼합액의 지속적인 사용은 검역해충의 방제에 있어서 친환경적이고 실제적으로 유효한 하나의 방법일 것이다.

Keywords

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