Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Effect of Methanol Extract of Dryopteris Crassirhizoma in Human Oral Cancer Cells

관중 메탄올추출물이 구강암세포주에 미치는 영향

  • Jang, Boonsil (Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Chonbuk National University) ;
  • Oh, Se-Jun (Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Chonbuk National University) ;
  • Shin, Ji-Ae (Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Chonbuk National University) ;
  • Lee, Hang-Eun (Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Chonbuk National University) ;
  • Jeon, Jae-Gyu (Department of Preventive Dentistry, School of Dentistry, Institute of Oral Bioscience, Chonbuk National University) ;
  • Cho, Sung-Dae (Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Chonbuk National University)
  • 장분실 (전북대학교 치의학전문대학원 구강병리학교실) ;
  • 오세준 (전북대학교 치의학전문대학원 구강병리학교실) ;
  • 신지애 (전북대학교 치의학전문대학원 구강병리학교실) ;
  • 이행은 (전북대학교 치의학전문대학원 구강병리학교실) ;
  • 전재규 (전북대학교 치의학전문대학원 구강생체과학 연구소) ;
  • 조성대 (전북대학교 치의학전문대학원 구강병리학교실)
  • Received : 2014.05.12
  • Accepted : 2014.08.18
  • Published : 2014.09.30
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Abstract

Dryopteris crassirhizoma is one of the naturally occurring substance wood ferns and is known for having anti-inflammatory, antiviral and anthelmintic activities. However, there is less report about its anticancer effect in human cancer cell lines. In the present study, the effect of methanol extract of dryopteris crassirhizoma (MEDC) on apoptosis in human oral cancer cell lines (MC3 and HN22 cells) was investigated. MEDC inhibited cell viability and induced apoptosis. MEDC significantly increased Bak and truncated Bid proteins in MC3 cells and elevated only truncated Bid compared to the control while other Bcl-2 family proteins were not altered. MEDC has anticancer activity by inducing apoptotic cell death through the regulation of either Bak or Bid. These findings suggest that its extract possibly may be used for treating oral cancer.

천연유래물질 중의 하나인 관중은 양치식물 고사리목으로 항 염증 작용, 항 바이러스 작용 및 구충작용과 자궁 수축 작용 같은 효능이 있다고 알려져 있지만, 종양에서의 구강암 예방효능에 대한 연구는 보고된 바가 없다. 본 연구에서는 사람 구강암세포주인 MC3와 HN22세포에서 관중 메탄올추출물의 증식억제 효능 및 관련 분자표적을 확인하고자 하였다. 관중메탄올추출물은 세포증식을 유의성 있게 억제하고, Western blot분석법, DAPI염색법 결과에서 알 수 있듯이 세포사멸을 유도하는 것으로 나타났다. 또한, MC3세포에서는 Bak의 발현을 농도의존적으로 증가시켰고, HN22세포에서는 Bid의 발현에 영향을 주어 truncation을 유도하였다. 하지만, Bak과 Bid 이외의 다른 Bcl-2 family에는 영향을 주지 못했다. 따라서, 이러한 결과를 종합해 볼 때, 관중메탄올추출물은 구강암에서 암 예방효능을 가진 잠재성 있는 천연추출물이 될 수 있을 것으로 사료된다.

Keywords

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