Synergic Antimicrobial Activity of Scutellariae Radix, Coptidis Rhizoma and Salicylic Acid Combination against Escherichia coli and Pseudomonas aeruginosa

대장균과 녹농균에 대항하는 황금과 황련 및 살리실산 조성물의 항균상승효과

  • 김수영 ((재)전주생물소재연구소) ;
  • 김지현 ((주)아토큐앤에이부설연구소) ;
  • 유강열 ((재)전주생물소재연구소) ;
  • 이현서 ((주)아토큐앤에이부설연구소) ;
  • 전인화 ((주)아토큐앤에이부설연구소) ;
  • 강현주 ((주)아토큐앤에이부설연구소) ;
  • 이정노 (한국폴리텍바이오대학) ;
  • 최병민 (원광대학교 의과대학 생화학교실) ;
  • 장선일 ((주)아토큐앤에이부설연구소)
  • Received : 2014.06.07
  • Accepted : 2014.07.07
  • Published : 2014.08.25

Abstract

Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Listeria. monocytogenes and Bacillus cereus are pathogenic bacteria that should not be detected in cosmetics and foodstuffs. Therefore, we first investigated the antimicrobial activities of extracts of Scutellariae Radix(SR), Coptidis Rhizoma (CR) and salicylic acid(SA) in these pathogenic microorganisms. Although SA has been known to exhibit anti-inflammation and antimicrobial activity against pathogenic microorganisms, a high concentration of SA may cause serious side effects such as skin redness, skin burning, peeling or tissue damage. Hence, we focused on diminishing side effects followed by treatment of a high concentration of SA and investigated whether the combinations of SA with various concentrations(25-400 mg/mL), SR and CR with a concentration(100 mg/mL) which did not show antimicrobial activity against E. coli and P. aeruginosa exhibited meaningful antimicrobial effect against both strains. In our results, the combinations of SA with the lowest concentration(25 mg/mL), SR(100 mg/mL) and CR(100 mg/mL) exhibited significant antimicrobial activity against E.coli in comparison to SA alone(25 mg/mL) showing no antimicrobial activity. Moreover, the combinations of SA (100 mg/mL), SR and CR showed seven times higher antimicrobial activity against E. coli than SA alone(100 mg/mL) and exhibited a significant antimicrobial activity in comparison to ampicilin (p<0.05). The combinations of SA(100 mg/mL), SR and CR showed two times higher antimicrobial activity against P. aeruginosa than SA alone. Therefore, these results indicated that the combinations of SR, CR and SA with low concentration expressed the synergistic antimicrobial effect against E. coli and P. aeruginosa and showed great potential as an antimicrobial agent.

Keywords

References

  1. 김성조, 심우창, 천석조, 유의형, 김동철, 정명섭, 박경진, 송범호, 홍성삼, 이철수, 식중독 예방사업 중장기 계획 연구. 식품의약품안전청 연구결과보고서 pp 8-12, 2006.
  2. 윤기선, 이중정. 식중독균 미생물의 위해평가를 위한 종합계획 및 예측모델링 개발. 식품의약품안전청 연구결과보고서 pp 14-16, 2010.
  3. de Oliveira, C.S., Lira, B.F., Barbosa-Filho, J.M., Lorenzo, J.G., de Athayde-Filho, P.F. Synthetic approaches and pharmacological activity of 1,3,4-oxadiazoles: a review of the literature from 2000-2012. Molecules 17: 10192-10231, 2012. https://doi.org/10.3390/molecules170910192
  4. Alekshun, M.N., Levy, S.B. Molecular mechanisms of antibacterial multidrug resistance. Cell 128: 1037-1050, 2007. https://doi.org/10.1016/j.cell.2007.03.004
  5. Matsuda, S., Oh, K,, Hirayama, H. Pharmacokinetic and clinical evaluation of levofloxacin in obstetrical and gynecological field. Jpn J Antibiot 45: 285-292, 1992.
  6. 신계호, 곽일영, 이성원, 서경희, 문성준, 장이섭. 화학방부제배합량 감소를 위한 폴리올류의 항균, 방부영향력 연구. 대한화장품학회지 33: 111-115, 2007.
  7. Yimam, M., Brownell, L., Hodges, M., Jia, Q. Analgesic effects of a standardized bioflavonoid composition from Scutellaria baicalensis and Acacia catechu. J Diet Suppl 9: 155-165, 2012. https://doi.org/10.3109/19390211.2012.708713
  8. Kowalczyk, E., Krzesinski, P., Kura, M., Niedworok, J., Kowalski, J., Blaszczyk, J. Pharmacological effects of flavonoids from Scutellaria baicalensis. Przegl Lek 63: 95-96, 2006.
  9. Ma, B.L, Ma, Y.M. Pharmacokinetic properties, potential herb-drug interactions and acute toxicity of oral Rhizoma coptidis alkaloids. Expert Opin Drug Metab Toxicol 9: 51-61, 2013. https://doi.org/10.1517/17425255.2012.722995
  10. Yun, B.Y., Zhou, L., Xie, K.P., Wang, Y.J., Xie, M.J. Antibacterial activity and mechanism of baicalein. Yao Xue Xue Bao 47: 1587-1592, 2012.
  11. Bae, J.H., Lee. M.J., Lee, S.M. Antimicrobialeffect of Cutellaria Baicalensis george extracts on Food-Borne pathogens. J Kor Micribiol Biotechnol 33: 35-40, 2005.
  12. Lee, J.H., Stein, B.D. Antimicrobial activity of a combination of Mume fructus, Schizandrae fructus, and Coptidis rhizoma on enterohemorrhagic Escherichia coli O26, O111, and O157 and its effect on Shiga toxin releases. Foodborne Pathog Dis 8: 643-646, 2011. https://doi.org/10.1089/fpd.2010.0710
  13. 정윤혜. 황련 발효추출물의 항균효과 및 항산화활성에 관한 연구. 중부대학교 박사학위논문 pp 31-56, 2009.
  14. Choi, U.K., Kim, M.H., Lee, N.H. Optimization of antibacterial activity by Gold-Thread (Coptidis Rhizoma Franch) against Streptococcus mutans using evolutionary operation-factorial design technique. J Microbiol Biotechnol 17: 1880-1884, 2007.
  15. Mahdi, J.G., Mahdi, A.J., Mahdi, A.J., Bowen, I.D. The historical analysis of aspirin discovery, its relation to the willow treeand antiproliferative and anticancer potential. Cell Prolif 39: 147-155, 2006. https://doi.org/10.1111/j.1365-2184.2006.00377.x
  16. Chen, Z., Zheng, Z., Huang, J., Lai, Z., Fan, B. Bioosynthesis of salicylic acid in plants. Plant Signal Behav 4: 493-496, 2009. https://doi.org/10.4161/psb.4.6.8392
  17. Eisen, D.P. Manifold beneficial effects of acetyl salicylic acid and nonsteroidal anti-inflammatory drugs on sepsis. Intensive Care Med 38: 1249-1257, 2012. https://doi.org/10.1007/s00134-012-2570-8
  18. 배지현, 이명진, 이선미. 황금(Cutellaria baicalensis George) 추출물의 식중독성 미생물에 대한 항균효과. 한국미생물.생명공학회지 33: 35-40, 2005.
  19. 최미숙. 황금, 황련, 황백 (Scutellaria baicalensis, Coptis chinensis, Phellodendron amurense) 추출물의 항균효과. 숭실대학교 석사학위 논문 pp 14-17, 2009.
  20. 김은경. 식중독균에 대한 식물 추출물의 항균시너지 효과. 원광대학교 석사학위논문 pp 10-20, 2012.
  21. Christoper, T.D. Price, Ian, R.L., John, E.G. The effects od salicylate on bacteria. Int J Biochem Cell Biol 32: 1029-1043, 2000. https://doi.org/10.1016/S1357-2725(00)00042-X