Journal of Microbiology and Biotechnology

  • 제24권8호
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  • Pages.1109-1117
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  • 2014
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Synthesis of Chlorogenic Acid and p-Coumaroyl Shikimates from Glucose Using Engineered Escherichia coli

  • Cha, Mi Na (Department of Bioscience and Biotechnology, Bio, Molecular Informatics Center, Konkuk University) ;
  • Kim, Hyeon Jeong (Department of Bioscience and Biotechnology, Bio, Molecular Informatics Center, Konkuk University) ;
  • Kim, Bong Gyu (Department of Forest Resources, Gyeongnam National University of Science and Technology) ;
  • Ahn, Joong-Hoon (Department of Bioscience and Biotechnology, Bio, Molecular Informatics Center, Konkuk University)
  • 투고 : 2014.03.13
  • 심사 : 2014.04.27
  • 발행 : 2014.08.28
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초록

Chlorogenic acid and hydroxylcinnamoyl shikimates are major dietary phenolics as well as antioxidants, with recently discovered biological, activities including protection against chemotheraphy side effects and prevention of cardiovascular disease and cancer. Certain fruits and vegetables produce these compounds, although a microbial system can also be utilized for synthesis of chlorogenic acid and hydroxylcinnamoyl shikimates. In this study, we engineered Escherichia coli to produce chlorogenic acid and p-coumaroyl shikimates from glucose. For the synthesis of chlorogenic acid, two E. coli strains were used; one strain for the synthesis of caffeic acid from glucose and the other strain for the synthesis of chlorogenic acid from caffeic acid and quinic acid. The final yield of chlorogenic acid using this approach was approximately 78 mg/l. To synthesize p-coumaroyl shikimates, wild-type E. coli as well as several mutants were tested. Mutant E. coli carrying deletions in three genes (tyrR, pheA, and aroL) produced 236 mg/l of p-coumaroyl shikimates.

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참고문헌

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