The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Selection and a 3-Year Field Trial of Sorangium cellulosum KYC 3262 Against Anthracnose in Hot Pepper

  • Yun, Sung-Chul (Department of Biomedical Sciences, Sun Moon University)
  • Received : 2014.01.14
  • Accepted : 2014.04.06
  • Published : 2014.09.01
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Abstract

KYC 3262 was selected as a biocontrol agent against anthracnose on hot pepper from 813 extracts of myxobacterial isolates. Dual culture with Colletotrichum acutatum and 813 myxobacterial extracts was conducted, and 19 extracts were selected that inhibited germination and mycelial growth of C. acutatum. All selections were Sorangium cellulosum, which are cellulolytic myxobacteria from soil. With the infection bioassay on detached fruits in airtight containers, KYC 3262, KYC 3512, KYC 3279, and KYC 3584 were selected. The listed four myxobacteria were cultured in CSG/1 liquid media, and harvested filtrates were sprayed on the infected fruits. KYC 3262 was selected from the studies of attached fruit in a greenhouse study. KYC 3262 filtrate was applied for 3 years (from 2011 to 2013) in a field study in Asan, Republic of Korea. Control values of the KYC 3262 in the field were 31%, 89%, and 82% in 2011, 2012, and 2013, whereas values of the fungicide spray treatment were 19%, 97%, and 91%, respectively. Yields (kg/20 plants) of the KYC 3262 were 2.66 kg and 18.6 kg in 2011 and 2013, respectively, and those of the fungicide treatment were 2.0 kg and 20.2 kg, in 2011 and 2013, respectively.

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References

  1. Ahn, J. W. 2009. Spirodienal C, a new spiroketal produced by Sorangium cellulosum (Myxobacteria). Bull. Korean Chem. Soc. 30:1218-1220. https://doi.org/10.5012/bkcs.2009.30.5.1218
  2. Bartlett, D. W., Clough, J. M., Godwin, J. R., Hall, A. A., Hamer, M. and Dobrzanski, B. P. 2002. The strobilurin fungicides. Pest Manag. Sci. 58:649-662. https://doi.org/10.1002/ps.520
  3. Bull, C. T., Shetty, K. G. and Subbarao, K. V. 2002. Interactions between myxobacteria, plant pathogenic fungi, and biocontrol agents. Plant Dis. 86:889-896. https://doi.org/10.1094/PDIS.2002.86.8.889
  4. Choi, Y. H., Kim, H. T., Kim, J. C., Jang, K. S., Cho, K. Y. and Choi, G. J. 2006. In Vitro antifungal activities of 13 fungicides against pepper anthracnose fungi. Kor. J. Pest. Sci. (in Korean) 10:36-42.
  5. Chung, J. W., Lee, C. Y., Yun, S. C. and Cho, K. Y. 2008. Screening of myxobacteria inhibiting the growth of Collectotrichum acutatum causing anthracnose on pepper. Kor. J. Microbiol. Biotechnol. (in Korean) 36:21-27.
  6. Collange, B., Navarrete, M., Peyre, G., Mateille, T. and Tchamitchian, M. 2011. Root-knot nematode (Meloidogyne) management in vegetable crop production: The challenge of an agronomic system analysis. Crop Prot. 30:1251-1262. https://doi.org/10.1016/j.cropro.2011.04.016
  7. Dawid, W. 2000. Biology and global distribution of myxobacteria in soils. FEMS Microbiol. Rev. 24:403-427. https://doi.org/10.1111/j.1574-6976.2000.tb00548.x
  8. Gerth, K., Pradella, S., Perlova, O., Beyer, S and Muller, R. 2003. Myxobacteria: proficient producers of novel natural products with various biological activities-past and future biotechnological aspects with the focus on the genus Sorangium. J. Biotechnol. 102:233-253. https://doi.org/10.1016/S0168-1656(03)00030-0
  9. Hyun, H. S., Chung, J. W., Lee, H. B.,Youn, J. K., Lee, C. Y., Kim, D. H. and Cho, K. Y. 2009. Isolation of cellulosedegrading myxobacteria Sorangium cellulosum. Kor. J. Microbiol. (in Korean) 45: 48-53.
  10. Jung, H. I., Kim, K. K., Park, H. C., Lee, S. M., Kim, Y. G., Kim, H. S., Lee, C. Y. and Son, H. J. 2007. Isolation and characteristics of bacteria showing biocontrol and biofertilizing activities. Kor. J. Life Sci. (in Korean) 17:1682-1688.
  11. Kim, B. S., Anh, J. W. and Cho, K. Y. 1998. Isolation and identification of myxobacteria KR025 and searching of their bioactive compounds. Kor. J. Plant Pathol. (in Korean) 14:345-349.
  12. Kim, S. T., Ahn, M. I. and Yun, S. C. 2010. Evaluation of anthracnose forecaster of an integrated pest management system on hot pepper in the fields. Res. Plant Dis. (in Korean) 16:66-73. https://doi.org/10.5423/RPD.2010.16.1.066
  13. Kim, S. T. and Yun, S. C. 2011a. Biocontrol with Myxococcus sp. KYC 1126 against anthracnose in hot pepper. Plant Pathol. J. 27:156-163. https://doi.org/10.5423/PPJ.2011.27.2.156
  14. Kim, S. T. and Yun, S. C. 2011b. Selection of KYC 3270, a cellulolytic myxobacteria of Sorangium cellulosum, against several phytopathogens and a potential biocontrol agent against gray mold in stored fruit. Plant Pathol. J. 27:257-265. https://doi.org/10.5423/PPJ.2011.27.3.257
  15. Kim, Y. S., Bae, W. C. and Baek, S. J. 2003. Bioactive substances from myxobacteria. Kor. J. Microbiol. Biotechnol. 31:1-12.
  16. Kim, Y. S., Cho, Y. J., Kim, S. W., Kim, Y, J., Min, J. S. and Lee, Y. S. 2007. Studies of antagonistic bacteria against pepper anthracnose pathogen Collectotrichum species. Kor. J. Agr. Sci. 18:97-104.
  17. Krug, D., Zurek, G., Revermann, O., VosMichiel, V. G. J. and Muller, R. 2008. Discovering the hidden secondary metabolome of Myxococcus xanthus: a study of intraspecific diversity. App. Envir. Microbiol. 74:3058-3068. https://doi.org/10.1128/AEM.02863-07
  18. Lee, C., An, D., Lee, H. and Cho, K. 2013. Correlation between Sorangium cellulosum subgroups and their potential for secondary metabolite production. J. Microbiol. Biotechnol. 23:297-303. https://doi.org/10.4014/jmb.1210.10054
  19. Lim, T. H. 2005. Antifungal activity of Streptomyces griseofuscus 200401 against pathogens causing late blight and anthracnose on pepper. J. Pest. Sci. 9:102-107.
  20. Park, S. M., Jung, H. J. and Yu, T. S. 2006. Screening of an antagonistic bacterium for control of red-pepper anthracnose, Colletotrichum gloeosporioides. Kor. J. Life Sci. (in Korean) 16:420-426. https://doi.org/10.5352/JLS.2006.16.3.420
  21. Reichenbach, H. 2001. Myxobacteria, producers of novel bioactive substances. J. Industrial Microbiol. Biotech. 27:149-156. https://doi.org/10.1038/sj.jim.7000025
  22. Reichenbach, H. and Hofle, G. 1994. Discovery of a new antifungal mechanism of action: soraphen- an almost-success story. Scientific Annual Report, Gesellschaft fur Biotechnologische ForschungmbH. Braunschweig, pp. 5-22.
  23. Yu, M. Y. and Yun, S. C. 2007. Morphological and molecular identification of anthracnose Collectotrichum spp. from Chungnam field in 2005. Sun Moon J. Nat. Sci. 8:59-67.
  24. Zvkovic, S., Stojanovic, S., Ivanovic, Z.,Gavrilovic, V., Popovic, T. and Balaz, J. 2010. Screening of antagonistic activity of microorganisms against Colletotrichum acutatum and Colletotrichum gloeosporioides. Arch. Biol. Sci. Belgrade. 62:611-623. https://doi.org/10.2298/ABS1003611Z

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