Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Efficacy of callus induced from Ullengdo niche plants for skin protection

식물세포배양기술을 이용한 울릉도 자생식물의 세포주 개발 및 피부세포 효능

  • 최윤희 ((주)바이오에프디엔씨 안티에이징연구소) ;
  • 정해수 ((주)바이오에프디엔씨 안티에이징연구소) ;
  • 조문진 ((주)바이오에프디엔씨 안티에이징연구소) ;
  • 송미영 ((주)바이오에프디엔씨 안티에이징연구소) ;
  • 서효현 ((주)바이오에프디엔씨 안티에이징연구소) ;
  • 모상현 ((주)바이오에프디엔씨 안티에이징연구소)
  • Received : 2014.04.28
  • Accepted : 2014.08.07
  • Published : 2014.08.31
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Abstract

Many countries in the world have protected their native plants and utilized them as industrial materials in each country. In this aspect, it is increasingly important to develop cosmetics materials using native plants in Korea. Cosmetic materials have been developed with niche plants, such as Campanula takesimana Nakai, Dianthus superbus, Aster spathulifolius in Ullengdo, in which a specific plant distribution by distinct climate and environment was present. Water and ethanol extractions were performed from the calluses of Campanula takesimana Nakai, Dianthus superbus, Aster spathulifolius. HPLC analysis revealed different compositions and functions of effective elements in each ethanol extract. For example, all types of ethanol extracts showed an ability to heal wounds. In particular, the expression of the inflammation-related gene, COX-2, was decreased in response to the ethanol extracts of Dianthus superbus. These results indicate that the ethanol extracts from niche plants' calluses in Ullengdo are natural and environmentally-friendly compounds, and can be used as medical supplies associated with anti-inflammation and wound healing.

세계 각국은 자국의 자생식물을 활용하여 다양한 산업 활용에 집중하고 있으며 국내에서도 자생식물을 활용한 화장품 원료 소재 개발이 절실히 필요한 실정이다. 이에 본 연구는 특유의 기후와 환경에 의해 울릉도 특유의 식물분포를 갖는 울릉도 자생식물 후보군을 선정하고 이를 소재로 개발하여 화장품 원료로의 활용 가능성을 시험하였다. 섬초롱, 술패랭이, 해국에서 캘러스를 유도하고 대량배양 하였다. 대량배양하여 얻은 울릉도 자생식물 캘러스를 열수 및 에탄올 추출하여 COX-2 발현 비교, collagen 합성능 측정, wound healing 등 다양한 효능을 시험하였다. HPLC 분석을 통하여 열수 및 에탄올 추출물의 유효성분에 차이를 보임을 확인하였으며 또한 효능평가에서도 차이를 보였다. 술패랭이, 해국 캘러스 에탄올 추출물을 처리하였을 경우 항염 관련 단백질인 COX-2의 발현을 감소시키고, 모든 에탄올추출물은 wound healing assay를 통해 상처 치유능을 보임을 확인하였다. 이를 통해 울릉도 자생식물 캘러스 추출물이 자연친화적, 친환경적이며, 또한 국내 자생하는 식물을 활용한 소재로써 항염 및 상처치료 관련 제품에 기여할 것이다.

Keywords

References

  1. Kang, B. H. "Native plants and useful plants" Korean J. Seed Sci. 28, 12-16, 2008.
  2. Chung, G. Y., M. S. Park, B. M. Nam, K. N. Hong, J. Jang and C. H. Lee, "The regional folk plants in inland of Gyeongsangbuk-do(I)" Korean J. Plant Res. 23, 465-479, 2010.
  3. Im, H. T., H. H. Hong, H. D. Son, M. S. Park, B. M. Nam, B. K. Kwon, C. H. Lee and G. Y. Chung, "The usage of regional folk plants in Gyeongsangnam-do" Korean J. Plant Res. 24, 419-429, 2011. DOI: http://dx.doi.org/10.7732/kjpr.2011.24.4.419
  4. Kim, Mi-Seon, Kim, Kyoung-Hee, Yook, Hong-Sun, "Antioxidative Effects of Campanula takesimana Nakai Extract" J Korean Soc Food Sci Nutr. 41, 1331-1337, 2012. DOI: http://dx.doi.org/10.3746/jkfn.2012.41.10.1331
  5. In-Sik Shin, Mee-Young Lee, Hyekyung Ha, Woo-Young Jeon, Chang-Seob Seo and Hyeun-Kyoo Shin, "Dianthus superbus fructus suppresses airway inflammation by downregulating of inducible nitric oxide synthase in an ovalbumin-induced murine model of asthma" Journal of Inflammation. 9, 41-50, 2012. DOI: http://dx.doi.org/10.1186/1476-9255-9-41
  6. Eun-Jin Yang, Eun-Young Yim, Gwanpil Song, Gi-Ok Kim, Chang-Gu Hyun, "Inhibition of nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophages by Jeju plant extracts" Interdisc Toxicol. 2, 245-249, 2009.
  7. Shuai K, Liu B. "Regulation of JAK-STAT signalling in the immune system" Nat Rev Immunol. 3, 900-911, 2003. DOI: http://dx.doi.org/10.1038/nri1226
  8. Kim Y, Kim BH, Lee H, Jeon B, Lee YS, Kwon MJ et al. "Regulation of skin inflammation and angiogenesis by EC-SOD via HIF-1a and NF-kB pathways" Free Radic Biol Med. 51, 1985-1995, 2011. DOI: http://dx.doi.org/10.1016/j.freeradbiomed.2011.08.027
  9. E. M. Middleton and A. H. Teramura, "The role of flavonol glycosides and carotenoids in protecting soybean from ultraviolet-B damage" Plant Physiol. 103, 741-752, 1993. https://doi.org/10.1104/pp.103.3.741
  10. van Meerloo J1, Kaspers GJ, Cloos J, "Cell sensitivity assays: the MTT assay" Methods Mol Biol. 731, 237-45, 2011. DOI: http://dx.doi.org/10.1007/978-1-61779-080-5_20
  11. Fotakis G, Timbrell JA, "In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride" Toxicol Lett. 160, 171-7, 2006. DOI: http://dx.doi.org/10.1016/j.toxlet.2005.07.001
  12. Potapovich AI1, Lulli D, Fidanza P, Kostyuk VA, De Luca C, Pastore S, Korkina LG, "Plant polyphenols differentially modulate inflammatory responses of human keratinocytes by interfering with activation of transcription factors NF${\kappa}$ B and AhR and EGFR-ERK pathway" Toxicol Appl Pharmacol. 255, 138-49, 2011. DOI: http://dx.doi.org/10.1016/j.taap.2011.06.007
  13. Atsushi Masamune, Shin Hamada, Kazuhiro Kikuta, Tetsuya Takikawa, Shin Miura, Eriko Nakano, Tooru Shimosegawa. "The angiotensin II type I receptor blocker olmesartan inhibits the growth of pancreatic cancer by targeting stellate cell activities in mice" Journal of Gastroenterology. 48, 602-609, 2013.
  14. Cho S, Kim HH, Lee MJ, Lee S, Park CS, Nam SJ, Han JJ, Kim JW, Chung JH, "Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo" J Lipid Res. 49, 1235-45, 2008. DOI: http://dx.doi.org/10.1194/jlr.M700581-JLR200
  15. Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood, Timothy J Mitchison, "A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods" BMC Biotechnology. 4, 21, 2004. DOI: http://dx.doi.org/10.1186/1472-6750-4-21
  16. Coomber BL, Gotlieb AI, "In vitro endothelial wound repair. Interaction of cell migration and proliferation" Arteriosclerosis. 10, 215-222, 1990. DOI: http://dx.doi.org/10.1161/01.ATV.10.2.215
  17. Lampugnani MG, "Cell migration into a wounded area in vitro" Methods Mol Biol. 96, 177-182, 1999.

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