한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제22권4호
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  • Pages.306-312
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  • 2014
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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인삼종자로부터 분리된 내생균의 동정과 식물생장 촉진 관련 활성의 평가

Identification of Endophytic Bacteria in Panax ginseng Seeds and Their Potential for Plant Growth Promotion

  • 엄유리 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김보라 (충북대학교 특용식물학과) ;
  • 정진주 (충북대학교 특용식물학과) ;
  • 정찬문 (충북대학교 특용식물학과) ;
  • 이이 (충북대학교 특용식물학과)
  • Um, Yurry (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Bo Ra (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Jeong, Jin Ju (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Chung, Chan Moon (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Lee, Yi (Department of Industrial Plant Science and Technology, Chungbuk National University)
  • 투고 : 2014.07.01
  • 심사 : 2014.08.06
  • 발행 : 2014.08.30
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초록

Endophytes are microorganisms that live in the internal tissues of plants without harming the host plants. In this symbiotic relationship, the host plants provide nutrients and shelter to the endophytes, in turn, endophytes can promote the growth of host plants and act as a biological control agents against plant pathogens. Plant-microbe interactions like this are noted for natural methods for sustainable agriculture and environmental conservation. However, in spite of the infinite potential, there are only a few reports on the endophytes present in ginseng. In this study, we isolated and identified the endophytes from Panax ginseng seeds and evaluated the biological activities (IAA production ability, nitrogen fixation ability, phosphate solubilization capacity, siderophore production ability, and antifungal activities) of the endophyte isolates. Eight different endophytes were identified by 16S rRNA sequencing. Most of the endophytes have antibiotic and plant growth promoting (PGP) activities. Particularly, PgSEB5-37E have the highest antibiotic activity, both PgSEB5-37B and PgSEB5-37H have high PGP traits such as an abilities to produce IAA, solubilize phosphate and fix nitrogen. These results indicated that the endophytes from P. ginseng seeds may have applicable value to many industries. In order to use the isolated endophytes, quantitative analysis and field tests are needed to be performed.

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참고문헌

  1. Arora NK, Kang SC and Maheshwari DK. (2001). Isolation of siderophore producing strains of Rhizobium meliloti and their biocontrol potential against Macrophomina phaseolina that causes charcoal rot of groundnut. Current Science. 81:673-677.
  2. Barac T, Taghavi S, Borremans B, Provoost A, Oeyen L, Colpaert JV, Vangronsveld J and van der Lelie D. (2004). Engineered endophytic bacteria improve phytoremediation of water-soluble, volatile, organic pollutants. Nature Biotechnology. 22:583-588. https://doi.org/10.1038/nbt960
  3. Bric JM, Bostock RM and Silverstone SE. (1991). Rapid in site assay for indole acetic acid production by bacteria immobilization on a nitrocellulose membrane. Applied and Environmental Microbiology. 57:535-538.
  4. Chatli AS, Beri V and Sidhu BS. (2008). Isolation and characterisation of phosphate solubilising microorganisms from the cold desert habitat of Salix alba Linn. in trans Himalayan region of Himachal Pradesh. Indian Journal of Microbiology. 48:267-273. https://doi.org/10.1007/s12088-008-0037-y
  5. Cho KM, Hong SY, Lee SM, Kim YH, Kahng GG, Lim YP, Kim H and Yun HD. (2007). Endophytic bacterial communities in ginseng and their antifungal activity against pathogens. Microbial Ecology. 54:341-351. https://doi.org/10.1007/s00248-007-9208-3
  6. de Siqueira VM, Conti R, de Araujo JM and Souza-Motta CM. (2011). Endophytic fungi from the medicinal plant Lippia sidoides Cham. and their antimicrobial activity. Symbiosis. 53:89-95. https://doi.org/10.1007/s13199-011-0113-7
  7. Di Battista-Leboeuf C, Benizri E, Corbel G, Piutti S and Guckert SPA. (2003). Distribution of Pseudomonas sp. populations in relation to maize root location and growth stage. Agronomie. 23:441-446. https://doi.org/10.1051/agro:2003017
  8. Doty SL. (2011). Growth-promoting endophytic fungi of forest trees. Forestry Science. 80:151-156. https://doi.org/10.1007/978-94-007-1599-8_9
  9. Glick BR. (2004). Bacterial ACC deaminase and the alleviation of plant stress. Advances in Applied Microbiology. 56:291-312. https://doi.org/10.1016/S0065-2164(04)56009-4
  10. Guo B, Wang Y, Sun X and Tang K. (2008). Bioactive natural products from endophytes: A review. Applied Biochemistry and Microbiology. 44:153-158.
  11. Ivanova EG, Doronina NV and Trotsenko YuA. (2001). Aerobic methylobacteria are capable of synthesizing auxins. Microbiology. 70:392-397. https://doi.org/10.1023/A:1010469708107
  12. Janda JM and Abbott SL. (2007). 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: Pluses, perils, and pitfalls. Journal of Clinical Microbiology. 45:2761-2764. https://doi.org/10.1128/JCM.01228-07
  13. Khan SA, Hamayun M, Kim HY, Yoon HJ, Lee IJ and Kim JG. (2009). Gibberellin production and plant growth promotion by a newly isolated strain of Gliomastix murorum. World Journal of Microbiology and Biotechnology. 25:829-833. https://doi.org/10.1007/s11274-009-9981-x
  14. Kumaran RS, Kim HJ and Hur BK. (2010). Taxol promising fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata. Journal of Bioscience and Bioengineering. 110:541-546. https://doi.org/10.1016/j.jbiosc.2010.06.007
  15. Lee HJ, Park KC, Lee SH, Bang KH, Park HW, Hyun DY, Kang SW, Cha SW and Chung IM. (2012). Screening of antifungal Bacillus spp. against Alternaria Blight pathogen (Alternaria panax) and Anthracnose pathogen(Colletotrichum gloeosporioides) of ginseng. Korean Journal of Medicinal Crop Science. 20:339-344. https://doi.org/10.7783/KJMCS.2012.20.5.339
  16. Lugtenberg B and Kamilova F. (2009). Plant-growth-promoting rhizobacteria. Annual Review of Microbiology. 63:541-556. https://doi.org/10.1146/annurev.micro.62.081307.162918
  17. Nguyen NL, Kim YJ, Hoang VA, Min JW, Liang ZQ and Yang DC. (2013). Bacillus ginsengisoli sp. nov., isolated from soil of a ginseng field. International Journal of Systematic and Evolutionary Microbiology 63:855-860. https://doi.org/10.1099/ijs.0.031740-0
  18. Ongena M, Jourdan E, Adam A, Paquot M, Brans A, Joris B, Arpigny JL and Thonart P. (2007). Surfactin and fengycin lipopeptides of Bacillus subtilis as elicitors of induced systemic resistance in plants. Environmental Microbiology. 9:1084-1090. https://doi.org/10.1111/j.1462-2920.2006.01202.x
  19. Park SY, Lee GA, Chang YK, Kim DH, Kim MS, Heo SJ, Jeong HN, Park KC, Cha SW and Song BH. (2013). Comparative analysis on major growth responses and characteristics of shoot and root of Panax ginseng C. A. Meyer with six different years old. Korean Journal of Medicinal Crop Science. 21:296-300. https://doi.org/10.7783/KJMCS.2013.21.4.296
  20. Park YH, Lee SG, Ahn DJ, Kwon TR, Park SU, Lim HS and Bae H. (2012). Diversity of fungal endophytes in various tissues of Panax ginseng Meyer cultivated in Korea. Journal of Ginseng Research. 36:211-217. https://doi.org/10.5142/jgr.2012.36.2.211
  21. Pandey P, Kang SC, Gupta CP and Maheshwari DK. (2005). Rhizosphere competent Pseudomonas aeruginosa grc1 produces characteristic siderophore and enhances growth of Indian mustard(Brassica campestris). Current Microbiology. 51:303-309. https://doi.org/10.1007/s00284-005-0014-1
  22. Qiu F, Huang Y, Sun L, Zhang X, Liu Z and Song W. (2007). Leifsonia ginsengi sp. nov., isolated from ginseng root. International Journal of Systematic and Evolutionary Microbiology. 57:405-408. https://doi.org/10.1099/ijs.0.64487-0
  23. Reyes I, Bernier L and Antoun H. (2002). Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicillium rugulosum. Microbial Ecology. 44:39-48. https://doi.org/10.1007/s00248-002-1001-8
  24. Ryan RP, Germaine K, Franks A, Ryan DJ and Dowling DN. (2008). Bacterial endophytes: Recent developments and applications. FEMS Microbiology Letters. 278:1-9. https://doi.org/10.1111/j.1574-6968.2007.00918.x
  25. Rye JS, Lee SD, Lee YH, Lee ST, Kim DK, Cho SJ, Park SR, Bae DW, Park KH and Yun HD. (2000). Screening and identification of an antifungal Pseudomonas sp. that suppresses balloon flower root rot caused by Rhizoctonia solani. Journal of Microbiology and Biotechnology. 10:435-440.
  26. Ryu CM, Farag MA, Hu CH, Reddy MS, Wei HX, Pare PW and Kloepper JW. (2003). Bacterial volatiles promote growth in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America. 100:4927-4932. https://doi.org/10.1073/pnas.0730845100
  27. Saitou N and Nei M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. 4:406-425.
  28. Sa T and Chauhan PS. (2009). Research trends on plant associated beneficial bacteria as biofertilizers for sustainable agriculture: An overview. Korean Journal of Soil Science and Fertilizer. Special Issue. 20-28.
  29. Schulz B and Boyle C. (2005). The endophyte continuum. Mycological Research. 109:661-686. https://doi.org/10.1017/S095375620500273X
  30. Schwyn B and Neilands JB. (1987). Universal chemical assay for the detection and determination of siderophores. Annual Review of Biochemistry. 160:47-56. https://doi.org/10.1016/0003-2697(87)90612-9
  31. Taghavi S, Barac T, Greenberg B, Borremans B, Vangronsveld J and van der Lelie D. (2005). Horizontal gene transfer to endogenous endophytic bacteria from poplar improves phytoremediation of toluene. Applied and Environmental Microbiology. 71:8500-8505. https://doi.org/10.1128/AEM.71.12.8500-8505.2005
  32. Tan RX and Zou WX. (2001). Endophytes: A rich source of functional metabolites. Natural Product Reports. 18:448-459. https://doi.org/10.1039/b100918o
  33. Thimann KV. (1937). On the nature of inhibition caused by auxin. American Journal of Botany. 24: 407-412. https://doi.org/10.2307/2436422
  34. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F and Higgins DG. (1997). The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research. 25:4876-4882. https://doi.org/10.1093/nar/25.24.4876
  35. Wu H, Yang HY, You XL and Li YH. (2013). Diversity of endophytic fungi from roots of Panax ginseng and their saponin yield capacities. SpringerPlus(SpringerOpen Journal). 2:107.

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