Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anti-cancer Effects of Oldenlandia diffusa, Cremastra appendiculata and Fritillaria thunbergii on MCF-7 Cells

MCF-7 인체 유방암 세포에서 백화사설초(白花蛇舌草), 산자고(山慈姑), 절패모(浙貝母)의 항암 효과

  • Jin, Myung Ho (Department of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University) ;
  • Hong, Sang Hoon (Department of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University) ;
  • Park, Cheol (Department of Molecular Biology, Dong-Eui University) ;
  • Choi, Yung Hyun (Department of Biochemistry, College of Korean Medicine, Dong-Eui University) ;
  • Park, Sang Eun (Department of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University)
  • 진명호 (동의대학교 한의과대학 한방내과) ;
  • 홍상훈 (동의대학교 한의과대학 한방내과) ;
  • 박철 (동의대학교 분자생물학과) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 박상은 (동의대학교 한의과대학 한방내과)
  • Received : 2014.03.13
  • Accepted : 2014.05.27
  • Published : 2014.06.25
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Abstract

Oldenlandia diffusa, Cremastra appendiculata and Fritillaria thunbergii are widely distributed in the Korea, China and Japan, and has been used in traditional medicine for various diseases, such as pharyngolaryngitis, tonsillitis, goiter and stomach ulcer. However, the anti-cancer activities in human breast cancer have not been clearly elucidated yet. In this study, it was compared the in vitro cytotoxic effects of single and complex treatment of O. diffusa, C. appendiculata and F. thunbergii. We treat human breast cancer MCF-7 cells with O. diffusa, C. appendiculata and F. thunbergii. And we evaluated viability, growth inhibition, morphological changes, apoptotic bodies formation, measurement of the cell cycle and formation of DNA fragmentation of these cells. It was found that single treatment of O. diffusa could inhibit the cell proliferation in human breast cancer MCF-7 cells. However, complex treatment of O. diffusa, C. appendiculata and F. thunbergii is weakly or not affect the cell proliferation of MCF-7 cells. And anti-proliferative effects of O. diffusa in MCF-7 cells was associated with G1 arrest of cell cycle. These findings suggest that O. diffusa may be a potential chemotherapeutic agent for the control of human breast cancer cells and further studies will be needed to identify the molecular mechanisms.

Keywords

References

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