Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Identification of Pork Contamination in Meatballs of Indonesia Local Market Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis

  • Erwanto, Yuny (Halal Products Research Center, Gadjah Mada University) ;
  • Abidin, Mohammad Zainal (Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University) ;
  • Muslim, Eko Yasin Prasetyo (Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University) ;
  • Sugiyono, Sugiyono (Faculty of Veterinary Medicine, Gadjah Mada University) ;
  • Rohman, Abdul (Halal Products Research Center, Gadjah Mada University)
  • Received : 2014.01.07
  • Accepted : 2014.04.15
  • Published : 2014.10.01
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Abstract

This research applied and evaluated a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using cytochrome b gene to detect pork contamination in meatballs from local markets in Surabaya and Yogyakarta regions, Indonesia. To confirm the effectiveness and specificity of this fragment, thirty nine DNA samples from different meatball shops were isolated and amplified, and then the PCR amplicon was digested by BseDI restriction enzyme to detect the presence of pork in meatballs. BseDI restriction enzyme was able to cleave porcine cytochrome b gene into two fragments (131 bp and 228 bp). Testing the meatballs from the local market showed that nine of twenty meatball shops in Yogyakarta region were detected to have pork contamination, but there was no pork contamination in meatball shops in Surabaya region. In conclusion, specific PCR amplification of cytochrome b gen and cleaved by BseDI restriction enzymes seems to be a powerful technique for the identification of pork presence in meatball because of its simplicity, specificity and sensitivity. Furthermore, pork contamination intended for commercial products of sausage, nugget, steak and meat burger can be checked. The procedure is also much cheaper than other methods based on PCR, immunodiffusion and other techniques that need expensive equipment.

Keywords

References

  1. Aida, A. A., Y. B. Che-Man, C. M. V. L. Wong, A. R. Raha, and R. Son. 2005. Analysis of raw meats and fats of pig using polymerase chain reaction for halal authentication. Meat Sci. 69:47-52. https://doi.org/10.1016/j.meatsci.2004.06.020
  2. Ballin, N. Z. 2010. Authentication of meat and meat products. Meat Sci. 86:577-587. https://doi.org/10.1016/j.meatsci.2010.06.001
  3. Bottero, M. T. and A. Dalmasso. 2011. Animal species identification in food products: Evolution of biomolecular methods. Vet. J. 190:34-38. https://doi.org/10.1016/j.tvjl.2010.09.024
  4. Chen, S. Y., Y. G. Yao, and Y. P. Liu. 2012. Species identification of ten common farm animals based on mitochondrial 12S rRNA gene polymorphisms. Anim. Biotechnol. 23:213-220. https://doi.org/10.1080/10495398.2012.696568
  5. Erwanto, Y., M. Z. Abidin, A. Rohman, and Sismindari. 2011. PCR-RFLP Using BseDI enzyme for pork authentication In sausage and nugget products. Media Peternakan J. Anim. Sci. Technol. 34:14-18. https://doi.org/10.5398/medpet.2011.34.1.14
  6. Erwanto, Y., M. Z. Abidin, Sismindari, and A. Rohman. 2012. Pig species identification in meatballs using polymerase chain reaction- restriction fragment length polymorphism for halal authentication. Int. Food Res. J. 19:901-906.
  7. Fajardo, V., I. Gonzales, I. Lopez-Calleja, I. Martin, and P. E. Hernandez. 2006. PCR-RFLP authentication of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries) and goat (Capra hircus). J. Agric. Food Chem. 54:1144-1150. https://doi.org/10.1021/jf051766r
  8. Girish, P. S., A. S. R. Anjaneyuhu, K. N. Viswas, B. M. Shivakumar, M. Anand, M. Patel, and B. Sharma. 2005. Meat species identification by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of mitochondrial 12S rRNA gene. Meat Sci. 70:107-112. https://doi.org/10.1016/j.meatsci.2004.12.004
  9. He, J., W. Xu, Y. Shang, P. Zhu, X. Mei, W. Tian, and K. Huang. 2013. Development and optimization of an efficient method to detect the authenticity of edible oils. Food Control 31:71-79. https://doi.org/10.1016/j.foodcont.2012.07.001
  10. Ong, S. B., M. I. Zuraini, W. G. Jurin, Y. K. Cheah, R. Tunung, L. C. Chai, Y. Haryani, F. M. Ghazali, and R. Son. 2007. Meat molecular detection: Sensitivity of polymerase chain reaction-restriction fragment length polymorphism in species differentiation of meat from animal origin. ASEAN Food J. 14:51-59.
  11. Rastogi, G., S. D. Mahesh, S. Walujkar, A. Kumar, M. S. Patole, and Y. S. Shouche. 2007. Species identification and authentication of tissues of animal origin mitochondrial and nuclear markers. Meat Sci. 76:666-674. https://doi.org/10.1016/j.meatsci.2007.02.006
  12. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: A laboratory manual. 2 ed. 3 Vol. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
  13. Sun, Y. L. and C. S. Lin. 2003. Establishment and application of a fluorescent polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identifying porcine, caprine and bovine meats. J. Agric Food Chem. 51:1771-1776. https://doi.org/10.1021/jf020860u
  14. Tanabe, S., E. Miyauchi, A. Muneshige, K. Mio, and C. Sato. 2007. PCR method of detecting pork in foods for verifying allergen labeling and for identifying hidden pork ingredients in processed foods. Biosci. Biotechnol. Biochem. 71:1663-1667. https://doi.org/10.1271/bbb.70075
  15. Unajak, S., P. Meesawat, K. Anyamaneeratch, D. Anuwareepong, K. Srikulnath, and K. Choowongkomon. 2011. Identification of species (meat and blood samples) using nested-PCR analysis of mitochondrial DNA. Afr. J. Biotechnol. 10:5670-5676.

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