Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Coencapsulation of L-Ascorbic Acid and α-Tocopherol in Ethosomes and Their Properties

친수성 L-ascorbic acid와 소수성 α-tocopherol을 모두 포집하는 ethosome의 특성

  • Lim, Yoon Mi (Department of Applied Chemistry, Dongduk Women's University) ;
  • Jun, Yoon Kyung (Department of Applied Chemistry, Dongduk Women's University) ;
  • Park, Seyeon (Department of Applied Chemistry, Dongduk Women's University) ;
  • Jin, Byung Suk (Department of Applied Chemistry, Dongduk Women's University)
  • 임윤미 (동덕여자대학교 자연과학대학 응용화학과) ;
  • 전윤경 (동덕여자대학교 자연과학대학 응용화학과) ;
  • 박세연 (동덕여자대학교 자연과학대학 응용화학과) ;
  • 진병석 (동덕여자대학교 자연과학대학 응용화학과)
  • Received : 2014.04.14
  • Accepted : 2014.06.02
  • Published : 2014.08.10
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Abstract

Coencapsulation of hydrophilic L-ascorbic acid and hydrophobic ${\alpha}$-tocopherol in ethosome vesicles was attempted and their properties were investigated in this study. The size of vesicles decreased with increasing concentration of L-ascorbic acid solution encapsulated in ethosome. The vesicle size and encapsulation efficiency of ethosomes increased slightly when ${\alpha}$-tocopherol was added into the HPC-forming liquid crystalline membrane of ethosome. However, the vesicle size increased highly and the encapsulation efficiency decreased abruptly at mixing ratios above 25 wt% due to the formation of an imperfect liquid crystalline structure within a vesicle membrane. It was observed that antioxidant activity was maintained for 5 weeks at $40^{\circ}C$ when L-ascorbic acid and ${\alpha}$-tocopherol were coencapsulated in ethosome. The L-ascorbic acid in ethosome was stable compared to that in aqueous solution under UV radiation.

친수성 아스코르빈산(L-ascorbic acid)과 소수성 토코페롤(${\alpha}$-tocopherol)을 모두 함유하는 ethosome 베시클을 제조하면서 이들의 특성을 살펴보았다. Ethosome에 포집되는 아스코르빈산 수용액 농도가 증가할수록 베시클 입자 크기는 작아졌다. 토코페롤은 HPC에 혼합되어 ethosome 베시클 액정막의 구성 성분이 되는데 토코페롤 혼합에 의해 베시클 입자크기와 아스코르빈산 수용액의 포집효율이 다소 증가하였다. 그러나 혼합비율이 25 wt% 이상이 되면 베시클 막이 불완전한 액정구조로 변하면서 입자크기는 크게 증가하고 포집효율은 크게 감소하였다. 아스코르빈산과 토코페롤이 함께 ethosome에 포집되어 있을 때 항산화 효능이 고온($40^{\circ}C$)에서도 5주간 안정하게 유지됨을 확인하였다. UV 조사 실험에서도 아스코르빈산은 수용액 상태로 있을 때에 비해 ethosome에 포집되어 있을 때 안정성이 향상되었다.

Keywords

References

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