한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제29권2호
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  • Pages.183-188
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  • 2014
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
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Exfoliative Vaginal Cytology and Serum Progesterone during the Estrous Cycle of Indigenous Ewes in Bangladesh

  • Zohara, Begum Fatema (Department of Medicine, Surgery and Obstetrics, Hajee Mohammad Danesh Science and Technology University) ;
  • Azizunnesa, Azizunnesa (Department of Medicine and Surgery, Chittagong Veterinary and Animal Sciences University) ;
  • Islam, Md. Faruk (Department of Medicine, Surgery and Obstetrics, Hajee Mohammad Danesh Science and Technology University) ;
  • Alam, Md. Golam Shahi (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Bari, Farida Yeasmin (Department of Surgery and Obstetrics, Bangladesh Agricultural University)
  • 투고 : 2014.02.17
  • 심사 : 2014.06.20
  • 발행 : 2014.06.30
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초록

A study was carried out on 16 indigenous ewes in Bangladesh in order to assess the reproductive physiology, the pattern of vaginal cell exfoliation and progesterone profiles during the estrous cycle period. The mean estrous cycle length and duration of estrus were $15.8{\pm}0.12$ days and $31.1{\pm}0.57$ h respectively. The exfoliated epithelial cells were categorized into parabasal, intermediate, superficial and keratinized and their relative occurrences. The percentages of parabasal, intermediate and superficial cell type during proestrus were similar. The percentage of superficial cell type during estrus was 61.7%, which was significantly (p<0.01) differ from other types of cells and stages of estrus cycle. Metoestrus was predominant with neutrophils in addition with other cell types. Dioestrus was dominated by neutrophils. On days 0 to 5 of the cycle the progesterone concentration was 0.09 to $1.6{\pm}0.07ng/ml$. The length of diestrus was 5~10 days with a range of mean progesterone level of $1.6{\pm}0.07$ to $2.8{\pm}0.11ng/ml$. Progesterone levels increased significantly (p<0.01) after Day 5 and maximum level was $2.8{\pm}0.11ng/ml$ observed on Day 10 of the estrous cycle. Thereafter it dropped rapidly to basal level of $0.11{\pm}0.04ng/ml$ on Day 0 (p<0.01). These results indicate that the pattern of exfoliation of vaginal cells along with progesterone concentration could be used to determine the reproductive stages of indigenous ewe.

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