한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제31권3호
  • /
  • Pages.258-261
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  • 2014
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

대리모에서 출생한 돼지에서 돼지 써코 바이러스 2형 감염

Porcine Circovirus Type 2 Infection in a Piglet Born from a Surrogate Mother

  • Lee, Su-Hyung (College of Veterinary Medicine, Seoul National University) ;
  • Kang, Sang-Chul (Optipharm) ;
  • Park, Jun-Won (College of Veterinary Medicine, Seoul National University) ;
  • Chung, Hee-Chun (College of Veterinary Medicine, Seoul National University) ;
  • Park, Bong-Kyun (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Dae-Yong (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Jae-Hoon (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
  • 심사 : 2014.04.10
  • 발행 : 2014.06.30
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초록

연구 시설에서 사육 중이던 4주령 수컷 돼지가 아무 전구 증상 없이 폐사된 상태로 발견되었다. 이 돼지는 이종 장기 이식 연구를 위하여 체세포 핵이식 후 대리모로부터 출산하였다. 핵 이식을 위한 난소는 연구시설 밖의 개인 양돈장에서 채취하였다. 병리조직학적으로 돼지의 심장에서는 림프구, 큰포식세포 및 다핵거대세포의 침윤, 심근 괴사 및 섬유화를 특징으로 하는 다병소성에서 연결성의 육아종성 심근염이 관찰되었다. 림프장기에서는 심한 림프구의 소실과 조직구 또는 다핵세포의 침윤을 보이고 있었다. 면역조직화학염색을 통하여 심장의 괴사된 심근세포, 큰포식세포 및 다핵거대세포와 림프 장기의 림프구소실 영역에서 큰포식세포 및 다핵세포에서 돼지 써코바이러스 2형(PCV-2)의 항원이 검출되었다. 유산 또는 사산된 돼지에서 PCV-2와 관련된 번식장애에서는 심근염이 자주 발생하는 상황이며, 이와 유사한 병변이 PCV-2에 감염된 본 증례의 4주령 돼지에서도 관찰되었다. 이 돼지에서 PCV-2의 감염은 양돈장에서 채취한 난소에 본 바이러스가 오염 또는 감염되어 발생한 것으로 사료된다.

A 4-week-old male piglet being maintained in a research facility was found dead without any previous clinical signs. The piglet had been born from a surrogate mother after somatic nuclear transfer as part of a xenotransplantation study. Ovaries for nuclear transfer were obtained from a private farm outside the research facility. Histopathologically, multifocal to coalescing granulomatous myocarditis was observed in the heart, characterized by infiltration of lymphocytes, macrophages and multinucleated giant cells, and by myocardial necrosis and fibrosis. Lymphoid tissues showed marked lymphoid depletion with infiltration by histiocytes or giant cells. Immunohistochemistry showed PCV-2 antigens in necrotic myocytes, macrophages and multinucleated giant cells in the heart, as well as in macrophages and giant cells in lymphoid depleted areas of lymphoid tissues. Reproductive failure associated with PCV-2 in aborted or stillborn piglets is frequently characterized by myocarditis, and similar lesions were observed in this 4-week-old piglet with PCV-2 infection. The PCV-2 infection in this piglet may have been due to contamination or infection of an ovary from the pig farm.

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참고문헌

  1. Allan GM, Mc Neilly F, Meehan BM, Kennedy S, Mackie DP, Ellis JA, Clark EG, Espuna E, Saubi N, Riera P, Botner A, Charreyre CE. Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. Vet Microbiol 1999; 66: 115-123. https://doi.org/10.1016/S0378-1135(99)00004-8
  2. Bielanski A, Larochelle R, Algire J, Magar R. Distribution of PCV-2 DNA in the reproductive tract, oocytes and embryos of PCV-2 antibody-positive pigs. Vet Rec 2004; 155: 597-598. https://doi.org/10.1136/vr.155.19.597
  3. Bielanski A, Larochelle R, Magar R. An attempt to render oocytes and embryos free from the porcine circovirus type 2 after experimental in vitro exposure. Can J Vet Res 2004; 68: 222-225.
  4. Halbur PG, Miller LD, Paul PS, Meng XJ, Huffman EL, Andrews JJ. Immunohistochemical identification of porcine reproductive and respiratory syndrome virus (PRRSV) antigen in the heart and lymphoid system of three-week-old colostrum-deprived pigs. Vet Pathol 1995; 32: 200-204. https://doi.org/10.1177/030098589503200218
  5. Huang C, Hung JJ, Wu CY, Chien MS. Multiplex PCR for rapid detection of pseudorabies virus, porcine parvovirus and porcine circoviruses. Vet Microbiol 2004; 101: 209-214. https://doi.org/10.1016/j.vetmic.2004.04.007
  6. Kang SC, Jung JY, Yang HS, Park BK, Kim DY, Kim JH. Detection of potentially xenozoonotic viruses in the porcine ovary in Korea. Korean J Vet Res 2009; 49: 215-220.
  7. Kono Y, Kanno T, Shimizu M, Yamada S, Ohashi S, Nakamine M, Shirai J. Nested PCR for detection and typing of porcine reproductive and respiratory syndrome (PRRS) virus in pigs. J Vet Med Sci 1996; 58: 941-946. https://doi.org/10.1292/jvms.58.10_941
  8. Lyoo KS, Park YH, Park BK. Prevalence of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and porcine parvovirus from aborted fetuses and pigs with respiratory problems in Korea. J Vet Sci 2001; 2: 201-207.
  9. Mikami O, Nakajima H, Kawashima K, Yoshii M, Nakajima Y. Nonsuppurative myocarditis caused by porcine circovirus type 2 in a weak-born piglet. J Vet Med Sci 2005; 67: 735-738. https://doi.org/10.1292/jvms.67.735
  10. Pittman JS. Reproductive failure associated with porcine circovirus type 2 in gilts. J Swine Health Prod 2008; 16: 144-148.
  11. Segales J. Porcine circovirus type 2 (PCV2) infections: clinical signs, pathology and laboratory diagnosis. Virus Res 2012; 164: 10-19. https://doi.org/10.1016/j.virusres.2011.10.007
  12. Sorden SD, Harms PA, Nawagitgul P, Cavanaugh D, Paul PS. Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue. J Vet Diagn Invest 1999; 11: 528-530. https://doi.org/10.1177/104063879901100607
  13. Tischer I, Rasch R, Tochtermann G. Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines. Zentralbl Bakteriol Orig A 1974; 226: 153-167.
  14. Vanderhallen H, Koenen F. Rapid diagnosis of encephalomyocarditis virus infections in pigs using a reverse transcription-polymerase chain reaction. J Virol Methods 1997; 66: 83-89. https://doi.org/10.1016/S0166-0934(97)02214-3
  15. West KH, Bystrom JM, Wojnarowicz C, Shantz N, Jacobson M, Allan GM, Haines DM, Clark EG, Krakowka S, McNeilly F, Konoby C, Martin K, Ellis JA. Myocarditis and abortion associated with intrauterine infection of sows with porcine circovirus 2. J Vet Diagn Invest 1999; 11: 530-532. https://doi.org/10.1177/104063879901100608
  16. Yang JS, Song DS, Kim SY, Lyoo KS, Park BK. Detection of porcine circovirus type 2 in feces of pigs with or without enteric disease by polymerase chain reaction. J Vet Diagn Invest 2003; 15: 369-373. https://doi.org/10.1177/104063870301500412