Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Porcine Circovirus Type 2 Infection in a Piglet Born from a Surrogate Mother

대리모에서 출생한 돼지에서 돼지 써코 바이러스 2형 감염

  • Lee, Su-Hyung (College of Veterinary Medicine, Seoul National University) ;
  • Kang, Sang-Chul (Optipharm) ;
  • Park, Jun-Won (College of Veterinary Medicine, Seoul National University) ;
  • Chung, Hee-Chun (College of Veterinary Medicine, Seoul National University) ;
  • Park, Bong-Kyun (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Dae-Yong (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Jae-Hoon (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
  • Accepted : 2014.04.10
  • Published : 2014.06.30
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Abstract

A 4-week-old male piglet being maintained in a research facility was found dead without any previous clinical signs. The piglet had been born from a surrogate mother after somatic nuclear transfer as part of a xenotransplantation study. Ovaries for nuclear transfer were obtained from a private farm outside the research facility. Histopathologically, multifocal to coalescing granulomatous myocarditis was observed in the heart, characterized by infiltration of lymphocytes, macrophages and multinucleated giant cells, and by myocardial necrosis and fibrosis. Lymphoid tissues showed marked lymphoid depletion with infiltration by histiocytes or giant cells. Immunohistochemistry showed PCV-2 antigens in necrotic myocytes, macrophages and multinucleated giant cells in the heart, as well as in macrophages and giant cells in lymphoid depleted areas of lymphoid tissues. Reproductive failure associated with PCV-2 in aborted or stillborn piglets is frequently characterized by myocarditis, and similar lesions were observed in this 4-week-old piglet with PCV-2 infection. The PCV-2 infection in this piglet may have been due to contamination or infection of an ovary from the pig farm.

연구 시설에서 사육 중이던 4주령 수컷 돼지가 아무 전구 증상 없이 폐사된 상태로 발견되었다. 이 돼지는 이종 장기 이식 연구를 위하여 체세포 핵이식 후 대리모로부터 출산하였다. 핵 이식을 위한 난소는 연구시설 밖의 개인 양돈장에서 채취하였다. 병리조직학적으로 돼지의 심장에서는 림프구, 큰포식세포 및 다핵거대세포의 침윤, 심근 괴사 및 섬유화를 특징으로 하는 다병소성에서 연결성의 육아종성 심근염이 관찰되었다. 림프장기에서는 심한 림프구의 소실과 조직구 또는 다핵세포의 침윤을 보이고 있었다. 면역조직화학염색을 통하여 심장의 괴사된 심근세포, 큰포식세포 및 다핵거대세포와 림프 장기의 림프구소실 영역에서 큰포식세포 및 다핵세포에서 돼지 써코바이러스 2형(PCV-2)의 항원이 검출되었다. 유산 또는 사산된 돼지에서 PCV-2와 관련된 번식장애에서는 심근염이 자주 발생하는 상황이며, 이와 유사한 병변이 PCV-2에 감염된 본 증례의 4주령 돼지에서도 관찰되었다. 이 돼지에서 PCV-2의 감염은 양돈장에서 채취한 난소에 본 바이러스가 오염 또는 감염되어 발생한 것으로 사료된다.

Keywords

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