Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Activation of Platelet Rich Plasma by Soluble Canine Small Intestinal Submucosa Gel and Bovine Thrombin

개 소장점막하 겔과 소 트롬빈을 이용한 혈소판풍부혈장의 활성화 연구

  • Lee, A-Jin (Departments of Veterinary Surgery, College of Veterinary Medicine, Konkuk University) ;
  • Lee, Changsun (Departments of Veterinary Surgery, College of Veterinary Medicine, Konkuk University) ;
  • Kim, Hyun (Departments of Veterinary Surgery, College of Veterinary Medicine, Konkuk University) ;
  • Chung, Dai-Jung (Departments of Veterinary Surgery, College of Veterinary Medicine, Konkuk University) ;
  • Do, Sun Hee (Departments of Veterinary Clinical Pathology, College of Veterinary Medicine, Konkuk University) ;
  • Kim, Hwi-Yool (Departments of Veterinary Surgery, College of Veterinary Medicine, Konkuk University)
  • 이아진 (건국대학교 수의과대학 수의외과학) ;
  • 이창선 (건국대학교 수의과대학 수의외과학) ;
  • 김현 (건국대학교 수의과대학 수의외과학) ;
  • 정다정 (건국대학교 수의과대학 수의외과학) ;
  • 도선희 (건국대학교 수의과대학 수의임상병리학) ;
  • 김휘율 (건국대학교 수의과대학 수의외과학)
  • Accepted : 2014.04.10
  • Published : 2014.06.30
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Abstract

This study evaluated the efficacy of soluble canine small intestinal submucosa gel in comparison to bovine thrombin in activating rabbit platelet rich plasma (PRP) by detecting growth factors. PRP from rabbits was activated by using soluble canine SIS gel, bovine thrombin, or both. The surface morphology of each group of samples was examined by scanning electron microscopy. The release of transforming growth factor (TGF)-${\beta}1$ from each set of samples was measured over 7 days using enzyme-linked immunosorbent assay. The PRP-canine SIS gel group exhibited the highest total amount of released TGF-${\beta}1$. However, there were no significant differences between any groups. The use of soluble type of canine SIS gel could be an effective alternative to bovine thrombin.

본 연구는 개 소장점막하 조직을 겔화시킨 것과 일반적으로 사용하는 소 트롬빈, 그리고 이 두 가지 요소를 혼합한 것을 혈소판풍부혈장의 활성화 인자로 적용하여, 혈소판에서 분비되는 성장인자를 7일간 in vitro 실험을 실시하였다. 또한 전자현미경으로 각 그룹 별 샘플의 표면을 관찰하여, 측정한 성장인자와의 상관관계를 연구하였다. 그 결과, 그룹별로 유의적인 차이는 존재하지 않았지만, 성장인자인 (TGF)-${\beta}1$이 가장 많이 분비된 그룹은 혈소판풍부혈장을 개 소장점막하조직 겔로 활성화시켰을 때였다. 본 연구 결과를 통해, 혈소판풍부혈장을 활성화하기 위한 요소로 개소장점막하조직 겔은 소의 트롬빈을 대체할 만한 인자로 여겨지며, 추후 임상에서 다양하게 적용할 수 있을 것으로 기대된다.

Keywords

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