Mycobiology

The Korean Society of Mycology (한국균학회)
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Neuraminidase Inhibitors from the Fermentation Broth of Phellinus linteus

  • Hwang, Byung Soon (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Lee, Myeong-Seok (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Lee, Seung Woong (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Lee, In-Kyoung (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Seo, Geon-Sik (Korea National College of Agriculture and Fisheries) ;
  • Choi, Hwa Jung (Department of Beauty Science, Kwangju Women's University) ;
  • Yun, Bong-Sik (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University)
  • Received : 2014.04.02
  • Accepted : 2014.05.15
  • Published : 2014.06.30
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Abstract

During a search for neuraminidase inhibitors derived from medicinal fungi, we found that the fermentation broth of Phellinus linteus exhibited potent neuraminidase inhibitory activity. Through bioassay-guided fractionation, two active compounds were purified from the ethyl acetate-soluble portion of the fermentation broth of P. linteus. These structures were identified as inotilone (1) and 4-(3,4-dihydroxyphenyl)-3-buten-2-one (2) by spectroscopic methods. Compounds 1 and 2 inhibited H1N1 neuraminidase activity with $IC_{50}$ values of 29.1 and 125.6 $125.6{\mu}M$, respectively, in a dose-dependent manner. They also exhibited an antiviral effect in a viral cytopathic effect reduction assay using MDCK cells. These results suggest that compounds 1 and 2 from the culture broth of P. linteus would be good candidates for the prevention and therapeutic strategies towards viral infections.

Keywords

References

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