Mycobiology

The Korean Society of Mycology (한국균학회)
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Biocontrol of Fusarium Crown and Root Rot and Promotion of Growth of Tomato by Paenibacillus Strains Isolated from Soil

  • Xu, Sheng Jun (Department of Plant Science, Gangneung-Wonju National University) ;
  • Kim, Byung Sup (Department of Plant Science, Gangneung-Wonju National University)
  • Received : 2014.01.08
  • Accepted : 2014.05.28
  • Published : 2014.06.30
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Abstract

In this study, bacterial strains were isolated from soils from 30 locations of Samcheok, Gangwon province. Of the isolated strains, seven showed potential plant growth promoting and antagonistic activities. Based on cultural and morphological characterization, and 16S rRNA gene sequencing, these strains were identified as Paenibacillus species. All seven strains produced ammonia, cellulase, hydrocyanic acid, indole-3-acetic acid, protease, phosphatase, and siderophores. They also inhibited the mycelial growth of Fusarium oxysporum f. sp. radicis-lycopersici in vitro. The seven Paenibacillus strains enhanced a range of growth parameters in tomato plants under greenhouse conditions, in comparison with non-inoculated control plants. Notably, treatment of tomato plants with one identified strain, P. polymyxa SC09-21, resulted in 80.0% suppression of fusarium crown and root rot under greenhouse conditions. The plant growth promoting and antifungal activity of P. polymyxa SC09-21 identified in this study highlight its potential suitability as a bioinoculant.

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References

  1. Lugtenberg B, Kamilova F. Plant-growth-promoting rhizobacteria. Annu Rev Microbiol 2009;63:541-56. https://doi.org/10.1146/annurev.micro.62.081307.162918
  2. Kavamura VN, Santos SN, da Silva JL, Parma MM, Avila LA, Visconti A, Zucchi TD, Taketani RG, Andreote FD, de Melo IS. Screening of Brazilian cacti rhizobacteria for plant growth promotion under drought. Microbiol Res 2013;168:183-91. https://doi.org/10.1016/j.micres.2012.12.002
  3. Kumar P, Dubey RC, Maheshwari DK. Bacillus strains isolated from rhizosphere showed plant growth promoting and antagonistic activity against phytopathogens. Microbiol Res 2012;167:493-9. https://doi.org/10.1016/j.micres.2012.05.002
  4. Emmert EA, Handelsman J. Biocontrol of plant disease: a (Gram-) positive perspective. FEMS Microbiol Lett 1999;171:1-9. https://doi.org/10.1111/j.1574-6968.1999.tb13405.x
  5. Von der Weid I, Alviano DS, Santos AL, Soares RM, Alviano CS, Seldin L. Antimicrobial activity of Paenibacillus peoriae strain NRRL BD-62 against a broad spectrum of phytopathogenic bacteria and fungi. J Appl Microbiol 2003;95:1143-51. https://doi.org/10.1046/j.1365-2672.2003.02097.x
  6. Deng Y, Lu Z, Lu F, Wang Y, Bie X. Study on an antimicrobial protein produced by Paenibacillus polymyxa JSa-9 isolated from soil. World J Microbiol Biotechnol 2011;27:1803-7. https://doi.org/10.1007/s11274-010-0638-6
  7. Raza W, Yang W, Shen QR. Paenibacillus polymyxa: antibiotics, hydrolytic enzymes and hazard assessment. J Plant Pathol 2008;90:419-30.
  8. Ozbay N, Newman SE. Fusarium crown and root rot of tomato and control methods. Plant Pathol J 2004;3:9-18. https://doi.org/10.3923/ppj.2004.9.18
  9. Omar I, O'Neill TM, Rossall S. Biological control of fusarium crown and root rot of tomato with antagonistic bacteria and integrated control when combined with the fungicide carbendazim. Plant Pathol 2006;55:92-9. https://doi.org/10.1111/j.1365-3059.2005.01315.x
  10. Nishiguchi MK, Doukakis P, Egan M, Kizirian D, Phillips A, Prendini L, Rosenbaum HC, Torres E, Wyner Y, DeSalle R, et al. DNA isolation procedures. In: DeSalle R, Giribet G, Wheeler W, editors. Methods and tools in biosciences and medicine: techniques in molecular systematics and evolution. Basel: Birkhäuser Verlag; 2002. p. 249-87.
  11. Bric JM, Bostock RM, Silverstone SE. Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl Environ Microbiol 1991;57:535-8.
  12. Kumar RS, Ayyadurai N, Pandiaraja P, Reddy AV, Venkateswarlu Y, Prakash O, Sakthivel N. Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broad-spectrum antifungal activity and biofertilizing traits. J Appl Microbiol 2005;98:145-54. https://doi.org/10.1111/j.1365-2672.2004.02435.x
  13. Nautiyal CS. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Lett 1999;170:265-70. https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
  14. Dey R, Pal KK, Bhatt DM, Chauhan SM. Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of plant growth-promoting rhizobacteria. Microbiol Res 2004;159:371-94. https://doi.org/10.1016/j.micres.2004.08.004
  15. Wei G, Kloepper JW, Tuzun S. Induction of systemic resistance of cucumber to Colletotrichum orbiculare by select strains of plant growth-promoting rhizobacteria. Phytopathology 1991;81:1508-12. https://doi.org/10.1094/Phyto-81-1508
  16. Schwyn B, Neilands JB. Universal chemical assay for the detection and determination of siderophores. Anal Biochem 1987;160:47-56. https://doi.org/10.1016/0003-2697(87)90612-9
  17. Teather RM, Wood PJ. Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 1982;43:777-80.
  18. Rowe RC. Comparative pathogenicity and host ranges of Fusarium oxysporum isolates causing crown and root rot of greenhouse and field-grown tomatoes in North America and Japan. Phytopathology 1980;70:1143-8. https://doi.org/10.1094/Phyto-70-1143
  19. Ramamoorthy V, Viswanathan R, Raguchander T, Prakasam V, Samiyappan R. Induction of systemic resistance by plant growth promoting rhizobacteria in crop plants against pests and diseases. Crop Prot 2001;20:1-11. https://doi.org/10.1016/S0261-2194(00)00056-9
  20. Dhanasekaran D, Sivamani P, Panneerselvam A, Thajuddin N, Rajakumar G, Selvamani S. Biological control of tomato seedling damping off with Streptomyces sp. Plant Pathol J 2005;4:91-5. https://doi.org/10.3923/ppj.2005.91.95
  21. Lamsal K, Kim SW, Kim YS, Lee YS. Biocontrol of late blight and plant growth promotion in tomato using rhizobacterial isolates. J Microbiol Biotechnol 2013;23:897-904. https://doi.org/10.4014/jmb.1209.09069
  22. McSpadden Gardener BB. Ecology of Bacillus and Paenibacillus spp. in agricultural systems. Phytopathology 2004;94:1252-8. https://doi.org/10.1094/PHYTO.2004.94.11.1252
  23. Ribeiro CM, Cardoso EJ. Isolation, selection and characterization of root-associated growth promoting bacteria in Brazil Pine (Araucaria angustifolia). Microbiol Res 2012;167:69-78. https://doi.org/10.1016/j.micres.2011.03.003
  24. Rodríguez H, Fraga R. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 1999;17:319-39. https://doi.org/10.1016/S0734-9750(99)00014-2
  25. Hameeda B, Harini G, Rupela OP, Wani SP, Reddy G. Growth promotion of maize by phosphate-solubilizing bacteria isolated from composts and macrofauna. Microbiol Res 2008;163:234-42. https://doi.org/10.1016/j.micres.2006.05.009
  26. Perez E, Sulbaran M, Ball MM, Yarzabal LA. Isolation and characterization of mineral phosphate-solubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region. Soil Biol Biochem 2007;39:2905-14. https://doi.org/10.1016/j.soilbio.2007.06.017
  27. Siddiqui ZA. PGPR: prospective biocontrol agents of plant pathogens. In: Siddiqui ZA, editor. PGPR: biocontrol and biofertilization. Dordrecht: Springer; 2006. p. 111-42.
  28. Raza W, Hongsheng W, Qirong S. Response of Paenibacillus polymyxa to iron: alternations in cellular chemical composition and the production of fusaricidin type antimicrobial compounds. Braz Arch Biol Technol 2010;53:1145-54. https://doi.org/10.1590/S1516-89132010000500019
  29. Blumer C, Haas D. Mechanism, regulation, and ecological role of bacterial cyanide biosynthesis. Arch Microbiol 2000;173:170-7. https://doi.org/10.1007/s002039900127
  30. Minaxi LN, Yadav RC, Saxena J. Characterization of multifaceted Bacillus sp. RM-2 for its use as plant growth promoting bioinoculant for crops grown in semi arid deserts. Appl Soil Ecol 2012;59:124-35. https://doi.org/10.1016/j.apsoil.2011.08.001