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Inhibitory Effects of Filiform Acupuncture and Laser Acupuncture at the Points of Zhongzhu($TE_3$).Zulinqi($GB_{41}$) on Neuropathic Pain in the Tibial and Sural Nerve Injury-induced Rats

중저(中渚)($TE_3$).임입(臨泣)($GB_{41}$) 침자 및 레이저침이 백서의 Tibial Nerve와 Sural Nerve 유발 동통 억제에 미치는 영향

  • Lee, Ju Hee (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Dongshin University) ;
  • Lee, Dong Geun (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Dongshin University) ;
  • Lee, Ook Jae (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Dongshin University) ;
  • Lee, Sang Hyun (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Dongshin University) ;
  • Lee, Jung Hun (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Dongshin University) ;
  • Jeong, Joo Yong (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Dongshin University) ;
  • Cheong, Min Seong (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Dongshin University) ;
  • Yang, Tae Jun (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Dongshin University) ;
  • Kim, Seon Wook (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Dongshin University) ;
  • Cho, Myoung Rae (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Dongshin University)
  • 이주희 (동신대학교 한의과대학 침구의학교실) ;
  • 이동근 (동신대학교 한의과대학 침구의학교실) ;
  • 이욱재 (동신대학교 한의과대학 침구의학교실) ;
  • 이상현 (동신대학교 한의과대학 침구의학교실) ;
  • 이정훈 (동신대학교 한의과대학 침구의학교실) ;
  • 정주용 (동신대학교 한의과대학 침구의학교실) ;
  • 정민성 (동신대학교 한의과대학 침구의학교실) ;
  • 양태준 (동신대학교 한의과대학 침구의학교실) ;
  • 김선욱 (동신대학교 한의과대학 침구의학교실) ;
  • 조명래 (동신대학교 한의과대학 침구의학교실)
  • Received : 2014.05.26
  • Accepted : 2014.05.28
  • Published : 2014.06.20

Abstract

Objectives : The purpose of this study was to investigate the analgesic effects of filiform acupuncture and laser acupuncture at the points of $TE_3{\cdot}GB_{41}$ in neuropathic pain induced rats. Methods : Neuropathic pain in rats was induced by partial resection of the tibial and sural nerves. Three weeks after the neuropathic surgery, each of the experimental groups(AT, LAT, AT + LAT and LAT + AT) was injected at the $TE_3{\cdot}GB_{41}$ twice per week for three weeks. Results : 1. All of the experimental groups(AT, LAT, AT + LAT and LAT + AT) showed a significant decrease in the plantar withdrawal response of allodynia and the thermal allodynia as compared with the control group. During the early phase, the AT and AT + LAT groups have been marked as more significant than the LAT + AT and LAT groups. 2. The expression of c-Fos significantly decreased in the LAT and LAT + AT groups as compared with the control group. 3. The LAT + AT group showed a significant decrease in Bax as compared with the control group. In each experimental groups(AT, LAT, AT + LAT and LAT + AT), Bcl-2 increased and Bax/Bcl-2 ratio decreased as compared with the control group. 4. The LAT, AT + LAT and LAT + AT groups showed a significant increase in mGluR5 as compared with the control group. Conclusions : These results represented that the filiform acupuncture and laser acupuncture at the $TE_3{\cdot}GB_{41}$ exerted anti-apoptotic and neuroprotective effects on the model of neuropathic pain, thereby suggesting that they should be available for decreasing mechanical allodynia.

Keywords

References

  1. Merskey H, Bogduk N. Classification of chronic pain. 2nd ed. Seattle : IASP Press. 1994 : 209-14.
  2. Treede RD, Jensen TS, Campbell JN et al. Neuropathic pain: redefinition and a grading system for clinical and research purposes. Neurology. 2008 ; 70 : 1630-5. https://doi.org/10.1212/01.wnl.0000282763.29778.59
  3. Available at : http://www.iasp-pain.org/Content/ NavigationMenu/General ResourceLinks/PainDefini tions/default.htm. Accessed May 22, 2012.
  4. Woolf CJ, Mannion RJ. Neuropathic pain: aetiology, symptoms, mechanisms, and management. The Lancet. 1999 ; 353 : 1959-64. https://doi.org/10.1016/S0140-6736(99)01307-0
  5. Torrance N, Smith BH, Bennett MI, Lee AJ. The epidemiology of chronic pain of predominantly neuropathic origin. Results from a general population survey. J Pain. 2006 ; 7(4) : 281-9. https://doi.org/10.1016/j.jpain.2005.11.008
  6. Bouhassira D, Lanteri-Minet M, Attal N, Laurent B, Touboul C. Prevalence of chronic pain with neuropathic characteristics in the general population. Pain. 2008 ; 136(3) : 380-7. https://doi.org/10.1016/j.pain.2007.08.013
  7. Hecke O, Austin SK, Khan RA, Smith BH, Torrance N. Neuropathic pain in the general population: a systematic review of epidemiological studies. Pain. 2014 ; 155(4) : 654-62. https://doi.org/10.1016/j.pain.2013.11.013
  8. Colombo B, Annovazzi POL, Comi G. Medications for neuropathic pain: current trends. Neurological Sci. 2006 ; 27 : S183-9. https://doi.org/10.1007/s10072-006-0666-z
  9. Nanjing University of Chinese Medicine. The annotation of The Huang Di Nei Jing-So Moon. Shanghai : Shanghai Scientific and Technical Publishers. 1997 : 278, 305-12, 361, 652.
  10. Zhang Jing Yue. Lei Jing(Vol. 1). Seoul : Daesung Publisher. 1982 : 372-5.
  11. Nanjing University of Chinese Medicine. The annotation of The Huang Di Nei Jing-Young Chu. Shanghai : Shanghai Scientific and Technical Publishers. 1997 : 1, 96-113.
  12. Korean Acupuncture & Moxibustion Society. Acupuncture and moxibustion. 2nd ed. Gyeonggi : Jipmoondang. 2008 : 130-1.
  13. Kim YU. A study on the Su Hyel Lon of the Nan gyeong. The Journal of the Korea Institue of Oriental Medical Infomatics. 2009 ; 15(1) : 11-55.
  14. Korean Acupuncture & Moxibustion Medicine Society. Acupuncture and moxibustion medicine. Gyeonggi : Jipmoondang. 2012 : 16, 225-30.
  15. Jung JH, Yun DW, Na CS, Ryu CR, Yun YC, Cho MR. Effects of acupuncture at $SI_{3}$, $BL_{40}$, $SI_{3}$, $BL_{40}$ on neuropathic pain control and c-Fos protein expression in rats. The Journal of Korean Acupuncture and Moxibustion Society. 2004 ; 21(1) : 240-51.
  16. Lee DH, Youn DH, Na CS, Yoon YC. Effects of bee venom acupuncture injected at Hwando($GB_{30}$) on neuropathic pain in rats. The Journal of Korean Acupuncture and Moxibustion Society. 2005 ; 22(5) : 67-77.
  17. Chiang SY, Bang SP, Kim JH, Wei TS, Yun YC. Effects of bee venom acopuncture at Sinsu($BL_{23}$) and Daejangsu($BL_{25}$) in different concentration on neuropathic pain induced by tibial and sural nerve injury in rats. The Journal of Korean Acupuncture and Moxibustion Society. 2010 ; 27(5) : 13-24.
  18. Oh CL, Na CS, Ryu CR, Cho MR. Inhibitory effects of electroacupuncture & acupuncture at Hu-gye ($SI_{3}$), Wijung($BL_{40}$), Hu-gye($SI_{3}$) and Wijung($BL_{40}$) on neuropathic pain in rats. The Journal of Korean Acupuncture and Moxibustion Society. 2005 ; 22(1) : 77-90.
  19. Wei TS, Youn DH, Youn YC, Na CS. Comparative study of acupuncture and invasive laser acupuncture therapy at $SI_{3}$ . $BL_{40}$ on the tibial, sural nerve injury and L5 spinal nerve ligation model in rats. Korean Journal of Acupuncture. 2005 ; 22(2) : 9-24.
  20. Jung JH, Cho MR, Wei TS, Ryu CR. Effects of acupuncture, electro-acupuncture, low level He- Ne laser therapy at Oe-gwan($TE_{5}$) . Chogimup ($GB_{41}$) on L5 spinal nerve ligation model in rats. The Journal of Korean Acupuncture and Moxibustion Society. 2007 ; 24(5) : 137-50.
  21. Lambert GA, Mallos G, Zagami AS. Von Frey's hairs - a review of their technology and use - a novel automated von Frey device for improved testing for hyperalgesia. Journal of Neuroscience Methods. 2009 ; 177(2) : 420-26. https://doi.org/10.1016/j.jneumeth.2008.10.033
  22. Shir Y, Seltzer Z. Effects of sympathectomy in a model of causalgiform pain produced by partial sciatic nerve injury in rats. Pain. 1991 ; 45(3) : 309-20. https://doi.org/10.1016/0304-3959(91)90056-4
  23. Yalcin I, Charlet A, Freund-Mercier M, Barrot M, Poisbeau P. Differentiating thermal allodynia and hyperalgesia using dynamic hot and cold plate in rodents. Journal of Pain. 2009 ; 10(7) : 767-73.
  24. Woolf CJ. What is this thing called pain? Journal of Clinical Investigation. 2010 ; 120(11) : 3742-4. https://doi.org/10.1172/JCI45178
  25. Yoon DM. Neuropathic pain. The Korean Journal of Pain. 1995 ; 8(2) : 219-24.
  26. Finnerup NB, Sindrup SH, Jensen TS. The evidence for pharmacological treatment of neuropathic pain. Pain. 2010 ; 150 : 573-81. https://doi.org/10.1016/j.pain.2010.06.019
  27. Attal N, Cruccu G, Baron R et al. EFNS guidelines on pharmacological treatment of neuropathic pain: 2010 revision. Eur J Neurol. 2010 ; 17 : 1113-23. https://doi.org/10.1111/j.1468-1331.2010.02999.x
  28. Yun YC, Choi KJ, Chae WS, Na CS, Song HK. Effect of acupuncture(Hapkok, LI4) based on retaining time on pain in rats. The Journal of Korean Acupuncture and Moxibustion Society. 1998 ; 15(2) : 319-29.
  29. Ding Guang Di. The annotation of Treatise on the Pathogenesis and Manifestations of All Diseases. 1st ed. Beijing: People's Medical Publishing House. 1992 : 152.
  30. Kim HH, Nam DW, Lee SH. Fundamentals and research trend of laser acupuncture. The Journal of Korean Acupuncture and Moxibustion Society. 2009 ; 26(6) : 21-30.
  31. Na CS, Youn DH, Choi CH, Lee SH, Oh KH, Jeong SH. The effects of traditional acupuncture techniques and green laser acupuncture on the blood pressure in hypertensive rat induced by two kidney one clip. Korean Journal of Acupuncture. 2008 ; 25(2) : 199-210.
  32. Ihm SJ, Youn DH, Na CS. Effects of invasive low level laser acupuncture therapy(LLLAT) at $LU_{7}$, $LR_{5}$, $LU_{7}$ + $LR_{5}$ on the hyperlipemia rats induced by high fat diet. Korean Journal of Acupuncture. 2005 ; 22(3) : 1-15.
  33. Kim WI, Youn DH, Choi CH, Na CS. Effects of low level laser treatment at $LR_{2}$ and $LR_{8}$ acupoint on the liver damage induced in D-GalN in rats. Korean Journal of Acupuncture. 2012 ; 29(1) : 131-41.
  34. Youn JY, Kim YS, Youn DH et al. Comparative studies of effects of manual acupuncture, invasive laser acupuncture and laser skin irradiation at the Heart Jeonggyeok acupoint on the focal ischemia induced by intraluminal filament insertion in rats. Korean Journal of Acupuncture. 2010 ; 27(1) : 125-42.
  35. Lim JA, Chae WS, Lee SH, Jeong SH, Youn DH, Na CS. Effects of GaAlAs laser and acupuncture therapy at $BL_{40}$ on neuropathic pain in rats. Korean Journal of Acupuncture. 2011 ; 28(2) : 37-47.
  36. Kim YS, Lee JH, Lee SH, Na CS, Cho MR. Effects of electro and laser acupuncture treatment with $GB_{39}$ and $GB_{34}$ on neuropathic pain in rats induced by tibial and sural nerve ligation. The Journal of Korean Acupuncture and Moxibustion Medicine Society. 2013 ; 30(3) : 125-34.
  37. Korean Acupuncture & Moxibustion Society. Acupuncture and moxibustion. 1st ed. Gyeonggi : Jipmoondang. 2008 : 201, 249-50.
  38. Lee OJ, Yun MY, Kim HH, Jo NG. Clinical study on a case of cervical myelopathy. The Journal of Korean Acupuncture and Moxibustion Society. 2003 ; 20(4) : 220-9.
  39. Seo BM, Yun JS, Kim SU et al. Comparison of treatment effect between oriental medicine theraphy and oriental and western medicine combination theraphy on low back pain patients. The Journal of Korean Acupuncture and Moxibustion Society. 2003 ; 20(6) ; 140-7.
  40. Choi IH. A clinical study of tinnitus. The Journal of Oriental Medical Surgery, Ophthalmology & Otolaryngology. 2001 ; 14(2) : 134-45.
  41. Ha CH, Han SG, Cho MR, Lee BR, Ryu CR. A clinical study on 29 cases with stroke treated by common acupuncture therapy and scalp acupunture. The Journal of Korean Acupuncture and Moxibustion Society. 2001 ; 18(4) : 82-90.
  42. Kim HJ, Ryu CR, Cho MR. Effects of Joongjeo ($TE_{3}$) supplementation Aekmoon($TE_{2}$) draining on changes in cerebral blood flow and blood pressure in normal rats. The Journal of Korean Acupuncture and Moxibustion Society. 2008 ; 25(6) : 1-12.
  43. Lim MJ, Ryu CR, Cho MR. Effects of Imeup($GB_{41}$) supplementation and Tonggok($BL_{66}$) draining on changes in cerebral hemodynamics in normal rats. The Journal of Korean Acupuncture and Moxibustion Society. 2008 ; 25(5) : 1-10.
  44. Yoon CY. Function disease symptom and organ coordination of tri-energizer based on the materiality of tri-energizer. J Oriental Medical Classics. 2013 ; 26(2) : 1-7.
  45. Ernst E, Fialka V. Ice freezes pain? A review of the clinical effectiveness of analgesic cold therapy. Journal of Pain and Symptom Management. 1994 ; 9(1) : 56-9. https://doi.org/10.1016/0885-3924(94)90150-3
  46. Milde-Langosch K. The Fos family of transcription factors and their role in tumourigenesis. European Journal of Cancer. 2005 ; 41(16) : 2449-61. https://doi.org/10.1016/j.ejca.2005.08.008
  47. Hu E, Mueller E, Oliviero S, Papaioannou VE, Johnson R, Spiegelman BM. Targeted disruption of the c-fos gene demonstrates c-fos-dependent and -independent pathways for gene expression stimulated by growth factors or oncogenes. The EMBO Journal. 1994 ; 13(13) : 3094-103.
  48. Hunt SP, Pini A, Evan G. Induction of c-fos-like protein in spinal cord neurons following sensory stimulation. Nature. 1987 ; 328 : 632-4. https://doi.org/10.1038/328632a0
  49. Harris JA. Using c-fos as a neural marker of pain. Brain Research Bulletin. 1998 ; 45(1) : 1-8. https://doi.org/10.1016/S0361-9230(97)00277-3
  50. Choi JH. Bax protein in cancer treatment. Journal of the Korean Medical Association. 2007 ; 50(11) : 1016-22. https://doi.org/10.5124/jkma.2007.50.11.1016
  51. Novellis V, Siniscalco D, Galderisi U et al. Blockade of glutamate mGlu5 receptors in a rat model of neuropathic pain prevents early over-expression of pro-apoptotic genes and morphological changes in dorsal horn lamina II. Neuropharmacology. 2004 ; 46(4) : 468-79. https://doi.org/10.1016/j.neuropharm.2003.10.014
  52. Kim HK, Kim PJ, Baik SW, Kim IS, Chung KS. Molecular biologic study on tbe changes of glutamate receptor(mGluR5) in rat hippocampus after brain ischemia. The Korean Journal of Critical Care Medicine. 2000 ; 15(2) : 75-81.