Restorative Dentistry and Endodontics

  • 제39권2호
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  • Pages.89-94
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  • 2014
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  • 2234-7658(pISSN)
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  • 2234-7666(eISSN)
대한치과보존학회 (The Korean Academy of Conservative Dentistry)
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Cytotoxicity and physical properties of tricalcium silicate-based endodontic materials

  • Jang, Young-Eun (Department of Conservative Dentistry, Chonnam National University School of Dentistry and Dental Science Research Institute) ;
  • Lee, Bin-Na (Department of Conservative Dentistry, Chonnam National University School of Dentistry and Dental Science Research Institute) ;
  • Koh, Jeong-Tae (Department of Pharmacology and Dental Therapeutics, Chonnam National University School of Dentistry and Dental Science Research Institute) ;
  • Park, Yeong-Joon (Department of Dental Materials, Chonnam National University School of Dentistry and Dental Science Research Institute) ;
  • Joo, Nam-Eok (University of Michigan School of Dentistry) ;
  • Chang, Hoon-Sang (Department of Conservative Dentistry, Chonnam National University School of Dentistry and Dental Science Research Institute) ;
  • Hwang, In-Nam (Department of Conservative Dentistry, Chonnam National University School of Dentistry and Dental Science Research Institute) ;
  • Oh, Won-Mann (Department of Conservative Dentistry, Chonnam National University School of Dentistry and Dental Science Research Institute) ;
  • Hwang, Yun-Chan (Department of Conservative Dentistry, Chonnam National University School of Dentistry and Dental Science Research Institute)
  • 투고 : 2013.10.10
  • 심사 : 2013.12.05
  • 발행 : 2014.05.30
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초록

Objectives: The aim of this study was to evaluate the cytotoxicity, setting time and compressive strength of MTA and two novel tricalcium silicate-based endodontic materials, Bioaggregate (BA) and Biodentine (BD). Materials and Methods: Cytotoxicity was evaluated by using a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide (XTT) assay. Measurements of 9 heavy metals (arsenic, cadmium, chromium, copper, iron, lead, manganese, nickel, and zinc) were performed by inductively coupled plasma-mass spectrometry (ICP-MS) of leachates obtained by soaking the materials in distilled water. Setting time and compressive strength tests were performed following ISO requirements. Results: BA had comparable cell viability to MTA, whereas the cell viability of BD was significantly lower than that of MTA. The ICP-MS analysis revealed that BD released significantly higher amount of 5 heavy metals (arsenic, copper, iron, manganese, and zinc) than MTA and BA. The setting time of BD was significantly shorter than that of MTA and BA, and the compressive strength of BA was significantly lower than that of MTA and BD. Conclusions: BA and BD were biocompatible, and they did not show any cytotoxic effects on human periodontal ligament fibroblasts. BA showed comparable cytotoxicity to MTA but inferior physical properties. BD had somewhat higher cytotoxicity but superior physical properties than MTA.

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