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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Enhanced Production of Succinic Acid by Actinobacillus succinogenes using the Production Medium Supplemented with Recombinant Carbonic Anhydrases

재조합 탄산무수화 효소 첨가 생산배지를 이용한 Actinobacillus succinogenes 유래의 숙신산 생산성 향상

  • Park, Sang-Min (College of Biomedical Science, Kangwon National University) ;
  • Eum, Kyuri (College of Biomedical Science, Kangwon National University) ;
  • Kim, Sangyong (Korea Institute of Industrial Technology) ;
  • Jeong, Yong-Seob (Department of Food Science and Technology, Chunbuk National University) ;
  • Lee, Dohoon (Korea Institute of Industrial Technology) ;
  • Chun, Gie-Taek (College of Biomedical Science, Kangwon National University)
  • 박상민 (강원대학교 의생명과학대학 분자생명과학과) ;
  • 엄규리 (강원대학교 의생명과학대학 분자생명과학과) ;
  • 김상용 (한국생산기술연구원) ;
  • 정용섭 (전북대학교 식품공학과) ;
  • 이도훈 (한국생산기술연구원) ;
  • 전계택 (강원대학교 의생명과학대학 분자생명과학과)
  • Received : 2014.05.01
  • Accepted : 2014.05.22
  • Published : 2014.06.30
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Abstract

Succinic acid, a representative biomass-derived platform chemical, is a major fermentation product of Actinobacillus succinogenes. It is well known that carbon dioxide is consumed during the succinate fermentation, but the biochemical mechanism behind this phenomenon is not yet understood well. In this study, it was found that the addition of carbonic anhydrase (CA)s into media significantly enhances the succinic acid production by A. succinogenes during the fermentation supplied with carbon dioxide. It is likely that the (bi) carbonate produced by the CA activity from gaseous carbon dioxide is favoured by A. succinogenes for consumption and utilization. Therefore, the $MgCO_3$ requirement could be significantly reduced without compromising the succinate productivity. Furthermore, because of too high price of the commercial carbonic anhydrase, it was undertaken to economically overproduce a cyanobacterial carbonic anhydrase by the use of a recombinant Pichia pastoris. An expression vector system was constructed with the carbonic anhydrase gene PCR-cloned from Cyanobacterium Synechocystis sp., and introduced into P. pastoris for fermentation studies. About 95.9 g/L of succinic acid was produced in the production medium with 30 ppm of carbonic anhydrase, approximately 2 fold higher productivity compared to the parallel process with no supplementation of the enzyme. It is expected that this method can provide a valuable way of overcoming inefficiencies inherent in gas supply during $CO_2$-based bioprocesses like succinic acid fermentation.

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References

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