Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Production Yield Enhancement of Mycosporine-like amino acid(MAA)s in Transformed Microalgae Culture by Radiofrequency

형질전환 미세조류의 고주파 처리 배양을 통한 MAA 생산량 증가

  • Seo, Hyo Hyun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Song, Mi Young (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Kulkarni, Atul (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Suh, Sung-Suk (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology) ;
  • Lee, Taek-Kyun (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology) ;
  • Moh, Sang Hyun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.)
  • 서효현 (바이오에프디엔씨 안티에이징연구소) ;
  • 송미영 (바이오에프디엔씨 안티에이징연구소) ;
  • 아툴 쿨카르니 (바이오에프디엔씨 안티에이징연구소) ;
  • 서승석 (한국해양과학기술원 남해특성연구부) ;
  • 이택견 (한국해양과학기술원 남해특성연구부) ;
  • 모상현 (바이오에프디엔씨 안티에이징연구소)
  • Received : 2014.04.28
  • Accepted : 2014.06.12
  • Published : 2014.06.30
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Abstract

In sea water, microalgae are exposed to a range of critical environmental conditions. Microalgae are protected from UV-A radiation due to the presence of mycosporine like amino acids(MAAs). Owing to the UV-A absorption properties of MAAs, they are used widely as a UV protecting ingredient in cosmetics. Therefore, there is a need to increase the production yield of MAAs. This study investigated the production yield of MAAs in transformed microalgae by radiofrequency(RF) exposure. Initially, the Glut-1 gene was transformed to Chlamydomonas hedleyi microalgae as a glucose transporter. The biomass was enhanced after Glut-1 gene transformation. In addition, the MAAs production yield was increased during large scale production in bioreactors due to the RF treatment. Therefore, purified extracts of MAAs can be used as a sun block material in the cosmetic industrial field.

Mycosporine-like 아미노산(MAAs)은 UV 흡수물질이며, 다양한 해양생물들은 MAAs의 합성과 축적을 통하여 환경자외선의 직 간접적인 영향을 감소시키는 기능을 진화시켜 왔다. 이 연구에서는 미세조류, Chlamydomonas hedleyi에 포도당 전달 단백질인 Glucose transporter 1(Glut-1) 유전자를 pCAM1303 벡터에 도입한 형질전환체를 제작하여, 형질전환체의 바이오매스를 최대로 증가시킬 수 있는 최적의 Glucose 농도와 NH4Cl농도를 결정하고, 고주파(Radiofrequency) 발생장치를 활용한 바이오매스 증가와 함께 MAA를 대량 생산할 수 있는 배양 조건을 확립하였다. 연구결과 고주파 처리를 통한 형질전환 미세조류는 4.13 mg/L(MAAs/DCW)으로 3.23 mg/L(MAAs/DCW)의 고주파 처리 없이 배양한 형질전환체보다 효율이 증가하였다. 이러한 결과는 자외선 A 흡수물질을 인위적으로 증폭시킬 수 있어서, 대량배양한 후 MAAs물질을 분리 및 정제하여 피부자극성이 없는 친환경적인 자외선 차단 화장품 산업화에 크게 기여할 수 있음을 의미한다.

Keywords

References

  1. W. F. Vincent and S. Roy. "Solar ultraviolet-B radiation and aquatic primary production: damage, protection and recovery". Environ. Rev. 1, 1-12, 1993. DOI: http://dx.doi.org/10.1139/a93-001
  2. J. Jagger. "physiological effects of near-ultraviolet radiation on bacteria". Photochem. Photobiol. Rev. 7, 1-75, 1983. DOI: http://dx.doi.org/10.1007/978-1-4684-4505-3_1
  3. R. P. Sinha and D-P. Hader. "Photobiology and ecophysiology of rice field cyanobacteria". Photochem. Photobiol. 64, 887-896, 1996. DOI: http://dx.doi.org/10.1111/j.1751-1097.1996.tb01852.x
  4. R. P. Sinha, M. Klisch, A. Groniger, D-P. Hader. "Responses of aquatic algae and cyanobacteria to solar UV-B". Plant Ecol. 154, 221-236, 2001. DOI: http://dx.doi.org/10.1023/A:1012986500543
  5. R. P. Sinha, P. Richter, J. Faddoul, M. Braun, D.-P. Hader, Effects of UV and visible light on cyanobacteria at the cellular level, Photochem. Photobiol. Sci. 1 (2002) 553-559. DOI: http://dx.doi.org/10.1039/B203955A
  6. S. P. Singh, M. Klisch, R. P. Sinha and D-P. Hader. "Genome mining of mycosporine-like amino acid (MAA) synthesizing and non synthesizing cyanobacteria: a bioinformatics study". Genomics, 95, 120-128, 2010. DOI: http://dx.doi.org/10.1016/j.ygeno.2009.10.002
  7. E. M. Middleton and A. H. Teramura. "The role of flavonol glycosides and carotenoids in protecting soybean from ultraviolet-B damage". Plant Physiol. 103, 741-752, 1993. DOI: http://dx.doi.org/10.1104/ pp.103.3.741
  8. S. P. Singh, S. Kumari, R. P. Rastogi, K. L. Singh and R. P. Sinha. "Mycosporine-like amino acids (MAAs): chemical structure, biosynthesis and significance as UV-absorbing/screening compounds". Ind. J. Exp. Biol. 46, 7-17, 2008.
  9. C. S. Cockell and J. Knowland. "Ultraviolet radiation screening compounds". Biol. Rev. Camb. Philos. Soc. 74, 311-345, 1999. DOI: http://dx.doi.org/10.1017/S0006323199005356
  10. A. Oren and N. Gunde-Cimerman. "Mycosporines and mycosporine-like amino acids: UV protectants or multipurpose secondary metabolites?". FEMS Microbiol. Lett. 269, 1-10, 2007. DOI: http://dx.doi.org/10.1111/j.1574-6968.2007.00650.x
  11. H. Nakamura, J. Kobayashi and Y. Hirata. "Separation of mycosporine like amino acids in marine organisms using reversed-phase high performance liquid chromatography". J. Chromatogr. 250, 113-118, 1982. DOI: http://dx.doi.org/10.1016/S0021-9673
  12. A. Oren. "Mycosporine-like amino acids as osmotic solutes in a community of halophilic cyanobacteria". Geomicrobiol. J. 14, 231-240. 1997. DOI: http://dx.doi.org/10.1080/01490459709378046
  13. I. Yakovleva, R. Bhagooli, A. Takemura and M. Hidaka. "Differential susceptibility to oxidative stress of two scleractinian corals: antioxidant functioning of mycosporine-glycine". Comp. Biochem. Physiol. B 139, 721-730, 2004. DOI: http://dx.doi.org/10.1016/j.cbpc.2004.08.016
  14. F. de la Coba, J. Aguilera, F. L. Figueroa, M. V. de Gaalvez and E. Herrera. "Antioxidant activity of mycosporine-like amino acids isolated from three red macroalgae and one marine lichen". J. Appl. Phycol. 21, 161-169, 2009. DOI: http://dx.doi.org/10.1007/s10811-008-9345-1
  15. E. P. Balskus and C. T. Walsh. "The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria". Science. 329, 1653-1656, 2010. DOI: http://dx.doi.org/10.1126/science.1193637
  16. Dunlap WC, GA Rae, EW Helbling, VE Villafane and OHolm-Hanse. "UV-absorbing compounds in natural assemblages of Antarctic phytoplankton". Antact. J.U.S. 30:323-326, 1995.
  17. Favre-Bonvin J, J Bernillon, N Salin and N Arpin. "Biosynthesis of mycosporine: mycosporine glutaminol in Trichothecium roseum". Phytochemistry. 26, 2509-2514, 1987. DOI: http://dx.doi.org/10.1016/S0031-9422(00)83866-2
  18. Banaszak AT and RK Trench. "Effects of ultraviolet(UV) radiation on microalgae-invertebrate symbiosis". J. Exp. Mar. Biol. Ecol. 194, 233-250, 1995. DOI: http://dx.doi.org/10.1016/0022-0981(95)00072-0
  19. Carefoot TH, M Harris, BE Taylor, D Donovan and DKarentz. "Mycosporine-like amino acids: possible protection in eggs of the sea hare Aplysia dactylomela". Mar. Biol. 130, 389-396, 1998. DOI: http://dx.doi.org/10.1007/s002270050259