International Journal of Industrial Entomology and Biomaterials

  • 제28권2호
  • /
  • Pages.25-31
  • /
  • 2014
  • /
  • 3022-7542(pISSN)
  • /
  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
권호 표지
DOI QR코드

DOI QR Code

Detection of Insect Pathogen Serratia marcescens in Protaetia brevitarsis seulensis (Kolbe) from Korea

  • Kwak, Kyu-Won (Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Han, Myung-Sae (Department of Bio-fibers and Materials Science, Kyungpook National University) ;
  • Nam, Sung-Hee (Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Choi, Ji-Young (Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Lee, Seok-Hyun (Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Choi, Young-Cheol (Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Park, Kwan-Ho (Department of Agricultural Biology, National Academy of Agricultural Science)
  • 투고 : 2014.04.07
  • 심사 : 2014.05.18
  • 발행 : 2014.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Protaetia brevitarsis seulensis (Kolbe) is widely used in Korea, as a protein-rich, alternate, functional food with pharmacological benefits. In addition to anti-oxidant properties, the larvae of P. b. seulensis also show positive effects against hepatic disorder and diabetes; therefore, P. b. seulensis larvae are being reared on a large scale in Korea. We evaluated reared larvae of P. b. seulensis from Gyeong-gi in Korea. Using 16SrRNA PCR, electro-microscopy, and bioassay techniques, we found that the larvae harbored Spo-1, a bacterium identified as the insect pathogen Serratia marcescens. Therefore, we highlight the use of this insect as an alternate food and the need for its sanitary rearing conditions, as contamination may affect public health.

키워드

참고문헌

  1. Ajithkumar B (2003) Spore-forming Serratia marcescens subsp. sakuensis subsp. nov., isolated from a domestic wastewater treatment tank. Int J Syst Evol Microbiol 53(1):253-258 doi:10.1099/ijs.0.02158-0
  2. Arthur I. Aronson Wb, and Peter dunn (1986) Bacillus thuringiensis and related insect pathogens. Microbiol Rev:1-24
  3. Burnside CE (1928) A Septicemic condition of adult bees. J Econ Entomol 21, 379-386 https://doi.org/10.1093/jee/21.2.379
  4. Carol R. Lauzon TGB, Robert E. Sjogren and Ronald J. Prokopy (2003) Serratia marcescens as a bacterial pathogen of rhagoletis pomonella flies. Eur J Entomol 100:87-92 https://doi.org/10.14411/eje.2003.017
  5. Casper Flyg KKaJGB (1980) Insect pathogenic properties of serratia marcescens phage resistant mutants witha decreased resistance to cecropia immunity and a decreased virulence to drosophila. J Gen Microbiol 120:173-181
  6. El-Aasar A.M.; El-Sheikh TAA, Rafea, Heba S. and Ali S.H. (2013) Biological and biochemical effects of bacillus thuringiensis serratia marcescens and teflubenzuron and their sequential combined effects on cotton leafworm spodoptera littoralis. Egypt Acad J Biolog Sci 5(1):1-13
  7. Fedrigo GV, Campoy EM, Di Venanzio G, Colombo MI, Garcia Vescovi E (2011) Serratia marcescens is able to survive and proliferate in autophagic-like vacuoles inside non-phagocytic cells. PloS one 6(8):e24054 doi:10.1371/journal.pone.0024054
  8. Gerc AJ, Song L, Challis GL, Stanley-Wall NR, Coulthurst SJ (2012) The insect pathogen Serratia marcescens Db10 uses a hybrid nonribosomal peptide synthetase-polyketide synthase to produce the antibiotic althiomycin. PloS one 7(9):e44673 doi:10.1371/journal.pone.0044673
  9. Grimont F, Grimont PAD (2006) The Genus Serratia.219-244 doi:10.1007/0-387-30746-x_11
  10. Hurst MR, Beard SS, Jackson TA, Jones SM (2007a) Isolation and characterization of the Serratia entomophila antifeeding prophage. FEMS Microbiol Lett 270(1):42-8 doi:10.1111/j.1574-6968.2007.00645.x
  11. Hurst MR, Jones SM, Tan B, Jackson TA (2007b) Induced expression of the Serratia entomophila Sep proteins shows activity towards the larvae of the New Zealand grass grub Costelytra zealandica. FEMS Microbiol Lett 275(1):160-7 doi:10.1111/j.1574-6968.2007.00886.x
  12. Jang-Jih Lu C-LP, Shih-Yi Lee and Chih-Chieng Wan (2000) Use of pcr with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid. J Clin Microbiol 38(6):2076-2080
  13. Joyner J, Wanless D, Sinigalliano CD, Lipp EK (2014) Use of Quantitative Real-Time PCR for Direct Detection of Serratia marcescens in Marine and Other Aquatic Environments. Appl Environ Microbiol 80(5):1679-83 doi:10.1128/AEM.02755-13
  14. Jung J-A, et al. (2013) Specific Detection of Serratia marcescens Based on a PCR Assay and Antimicrobial Susceptibility of S. marcescens Isolated from Boar Semen. J Life Sci 23(9):1133-1139 doi:10.5352/jls.2013.23.9.1133
  15. Kwon E-Y, et al. (2013) Pre-treatment of the White-Spotted Flower Chafer (Protaetia brevitarsis) as an Ingredient for Novel Foods. Korean J Soc Food Sci & Nut 42(3):397-402 doi:10.3746/jkfn.2013.42.3.397
  16. Lauri Mikonranta JM, Minna Kaukoniitty and Dalial Freitak (2014) Insect immunity oral exposure to a bacterial pathogen elicits free radical response and protects from a recurring infection. Front Zool 11(23):1-7 https://doi.org/10.1186/1742-9994-11-1
  17. Martinez-Granero F, et al. (2014) Identification of flgZ as a Flagellar Gene Encoding a PilZ Domain Protein That Regulates Swimming Motility and Biofilm Formation in Pseudomonas. PloS one 9(2):e87608 doi:10.1371/journal.pone.0087608
  18. Miyanoshita A, et al. (1996) Isolation and characterization of a new member of the insect defensin family from a beetle, Allomyrina dichotoma. Biochem & Biophysic Res Comm 220(3):526-31 doi:10.1006/bbrc.1996.0438
  19. Mulcahy H, Sibley CD, Surette MG, Lewenza S (2011) Drosophila melanogaster as an animal model for the study of Pseudomonas aeruginosa biofilm infections in vivo. PLoS Pathol 7(10):e1002299 doi:10.1371/journal.ppat.1002299
  20. Murdoch SL, Trunk K, English G, Fritsch MJ, Pourkarimi E, Coulthurst SJ (2011) The opportunistic pathogen Serratia marcescens utilizes type VI secretion to target bacterial competitors. J Bacteriol 193(21):6057-69 doi:10.1128/JB.05671-11
  21. Nunez-Valdez ME, et al. (2008) Identification of a putative Mexican strain of Serratia entomophila pathogenic against root-damaging larvae of Scarabaeidae (Coleoptera). Appl & Environ Microbiol 74(3):802-10 doi:10.1128/AEM.01074-07
  22. Schwarz RHaH (2004) Serratia marcescens internalization and replication in human bladder epithelial cells. BMC Infect Dis 4
  23. Suh HJ, Kim SR, Lee KS, Park S, Kang SC (2010) Antioxidant activity of various solvent extracts from Allomyrina dichotoma (Arthropoda:Insecta) larvae. J Photochem Photobiol B. 99(2):67-73 doi:10.1016/j.jphotobiol.2010.02.005
  24. Tanada Y, Kaya HK (1994) Insect Pathology. Academic Press INC. Harcourt Brace Jovanovich, Pubrichers, San Diego. [Internet]. Available from: http://wiki.ceh.ac.uk [accessed on 27 October 2013].
  25. White G.F. (1923). Hornworm septicemia. J Agri Res 26:447-486

피인용 문헌

  1. A Biological Activity of Serratia marcescens Strains Isolated from Dead Larva of the Diamondback Moth, Plutella xylostella (Plutellidae, Lepidoptera) vol.20, pp.2, 2016, https://doi.org/10.7585/kjps.2016.20.2.152
  2. Comparing the mortality of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) caused by entomopathogenic bacteria and Serratia marcescens (Enterobacteriales: Enterobacteriaceae) vol.30, pp.2, 2015, https://doi.org/10.7852/ijie.2015.30.2.40
  3. 식물병원성 해충과 선충 방제의 새지평 I: 미생물 vol.23, pp.2, 2014, https://doi.org/10.5423/rpd.2017.23.2.114
  4. 유용 미생물을 이용한 발효굼벵이 추출물의 이화학적 특성 및 생리활성효과 vol.28, pp.7, 2018, https://doi.org/10.5352/jls.2018.28.7.827