The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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The Sensor Kinase GacS Negatively Regulates Flagellar Formation and Motility in a Biocontrol Bacterium, Pseudomonas chlororaphis O6

  • Kim, Ji Soo (Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Kim, Yong Hwan (Korea Institute of Planning & Evaluation for Technology on Food, Agriculture, Forestry & Fisheries) ;
  • Anderson, Anne J. (Department of Biology, Utah State University) ;
  • Kim, Young Cheol (Institute of Environmentally-Friendly Agriculture, Chonnam National University)
  • 투고 : 2013.11.07
  • 심사 : 2014.01.24
  • 발행 : 2014.06.01
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초록

The GacS/GacA two component system regulates various traits related to the biocontrol potential of plant-associated pseudomonads. The role of the sensor kinase, GacS, differs between strains in regulation of motility. In this study, we determined how a gacS mutation changed cell morphology and motility in Pseudomonas chlororaphis O6. The gacS mutant cells were elongated in stationary-phase compared to the wild type and the complemented gacS mutant, but cells did not differ in length in logarithmic phase. The gacS mutant had a two-fold increase in the number of flagella compared with the wild type strain; flagella number was restored to that of the wild type in the complemented gacS mutant. The more highly flagellated gacS mutant cells had greater swimming motilities than that of the wild type strain. Enhanced flagella formation in the gacS mutant correlated with increased expression of three genes, fleQ, fliQ and flhF, involved in flagellar formation. Expression of these genes in the complemented gacS mutant was similar to that of the wild type. These findings show that this root-colonizing pseudomonad adjusts flagella formation and cell morphology in stationary-phase using GacS as a major regulator.

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참고문헌

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피인용 문헌

  1. Proteomic Analysis of a Global Regulator GacS Sensor Kinase in the Rhizobacterium, Pseudomonas chlororaphis O6 vol.30, pp.2, 2014, https://doi.org/10.5423/PPJ.NT.02.2014.0012
  2. Regulation of GacA in Pseudomonas chlororaphis Strains Shows a Niche Specificity vol.10, pp.9, 2015, https://doi.org/10.1371/journal.pone.0137553
  3. Relationship of the CreBC two-component regulatory system and inner membrane protein CreD with swimming motility in Stenotrophomonas maltophilia vol.12, pp.4, 2017, https://doi.org/10.1371/journal.pone.0174704
  4. Impact of a Recombinant Biocontrol Bacterium, Pseudomonas fluorescens pc78, on Microbial Community in Tomato Rhizosphere vol.32, pp.2, 2016, https://doi.org/10.5423/PPJ.OA.08.2015.0172
  5. Genome-wide analysis of the FleQ direct regulon in Pseudomonas fluorescens F113 and Pseudomonas putida KT2440 vol.8, pp.1, 2018, https://doi.org/10.1038/s41598-018-31371-z