Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Polymorphism, Expression of Natural Resistance-associated Macrophage Protein 1 Encoding Gene (NRAMP1) and Its Association with Immune Traits in Pigs

  • Ding, Xiaoling (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Zhang, Xiaodong (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Yang, Yong (Anhui Antai Pig Breeding Co., LTD.) ;
  • Ding, Yueyun (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Xue, Weiwei (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Meng, Yun (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Zhu, Weihua (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Yin, Zongjun (College of Animal Science and Technology, Anhui Agricultural University)
  • Received : 2014.01.08
  • Accepted : 2014.04.14
  • Published : 2014.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Natural resistance-associated macrophage protein 1 encoding gene (NRAMP1) plays an important role in immune response against intracellular pathogens. To evaluate the effects of NRAMP1 gene on immune capacity in pigs, tissue expression of NRAMP1 mRNA was observed by real time quantitative polymerase chain reaction (PCR), and the results revealed NRAMP1 expressed widely in nine tissues. One single nucleotide polymorphism (SNP) (ENSSSCG00000025058: g.130 C>T) in exon1 and one SNP (ENSSSCG00000025058: g.657 A>G) in intron1 region of porcine NRAMP1 gene were demonstrated by DNA sequencing and PCR-RFLP analysis. A further analysis of SNP genotypes associated with immune traits including contain of white blood cell (WBC), granulocyte, lymphocyte, monocyte (MO), rate of cytotoxin in monocyte (MC) and $CD4^-CD8^+$ T lymphocyte subpopulations in blood was carried out in four pig populations including Large White and three Chinese indigenous breeds (Wannan Black, Huai pig and Wei pig). The results showed that the SNP (ENSSSCG00000025058: g.130 C>T) was significantly associated with level of WBC % (p = 0.031), MO% (p = 0.024), MC% (p = 0.013) and $CD4^-CD8^+$ T lymphocyte (p = 0.023). The other SNP (ENSSSCG00000025058: g.657 A>G) was significantly associated with the level of MO% (p = 0.012), MC% (p = 0.019) and $CD4^-CD8^+$ T lymphocyte (p = 0.037). These results indicate that the NRAMP1 gene can be regarded as a molecular marker for genetic selection of disease susceptibility in pig breeding.

Keywords

References

  1. Ates, O., L. Dalyan, B. Musellim, G. Hatemi, H. Turker, G. Ongen, V. Hamuryudan, and A. Topal-Sarikaya. 2009. NRAMP1 (SLC11A1) gene polymorphisms that correlate with autoimmune versus infectious disease susceptibility in tuberculosis and rheumatoid arthritis. Int. J. Immunogenet. 36: 15-19. https://doi.org/10.1111/j.1744-313X.2008.00814.x
  2. Blackwell, J. M., S. Searle, T. Goswami, and E. N. Miller. 2000. Understanding the multiple functions of Nramp1. Microbes Infect. 2:317-321. https://doi.org/10.1016/S1286-4579(00)00295-1
  3. Blackwell, J. M., S. Searle, H. Mohamed, and J. K. White. 2003. Divalent cation transport and susceptibility to infectious and autoimmune disease: Continuation of the Ity/Lsh/Bcg/Nramp1/ Slc11a1 gene story. Immunol. Lett. 85:197-203. https://doi.org/10.1016/S0165-2478(02)00231-6
  4. Canonne-Hergaux, F., S. Gruenheid, P. Ponka, and P. Gros. 1999. Cellular and subcellular localization of the Nramp2 iron transporter in the intestinal brush border and regulation by dietary iron. Blood 93:4406-4417.
  5. Capon, F., M. H. Allen, M. Ameen, A. D. Burden, D. Tillman, J. N. Barkerand, and R. C. Trembath. 2004. A synonymous SNP of the corneodesmosin gene leads to increased mRNA stability and demonstrates association with psoriasis across diverse ethnic groups. Hum. Mol. Genet. 13:2361-2368. https://doi.org/10.1093/hmg/ddh273
  6. Courville, P., R. Chaloupka, and M. F. Cellier. 2006. Recent progress in structure-function analyses of Nramp protondependent metal-ion transporters. Biochem. Cell Biol. 84:960-978. https://doi.org/10.1139/o06-193
  7. Crawley, A. M., B. Mallard, and B. N. Wilkie. 2005. Genetic selection for high and low immune response in pigs: Effects on immunoglobulin isotype expression. Vet. Immunol. Immunopathol. 108:71-76. https://doi.org/10.1016/j.vetimm.2005.07.006
  8. Gazouli, M., V. Atsaves, G. Mantzaris, M. Economou, G. Nasioulas, K. Evangelou, A. J. Archimandritis, and N. P. Anagnou. 2008. Role of functional polymorphisms of NRAMP1 gene for the development of Crohn's disease. Inflamm. Bowel. Dis. 14:1323-1330. https://doi.org/10.1002/ibd.20488
  9. Hellemans, J., G. Mortier, A. De Paepe, F. Speleman, and J. Vandesompele. 2007. qBase relative quantification framework and software for management and automated analysis of realtime quantitative PCR data. Genome Biol. 8:R19. https://doi.org/10.1186/gb-2007-8-2-r19
  10. He, X. M., M. X. Fang, Z. T. Zhang, Y. S. Hu, X. Z. Jia, D. L. He, S. D. Liang, Q. H. Nie, and X. Q. Zhang. 2013. Characterization of chicken natural resistance-associated macrophage protein encoding genes (Nramp1 and Nramp2) and association with salmonellosis resistance. Genet. Mol. Res. 12:618-630. https://doi.org/10.4238/2013.January.30.5
  11. Hedges, J. F., E. Kimmel, D. T. Snyder, M. Jerome, and M. A. Jutila. 2013. Solute carrier 11A1 is expressed by innate lymphocytes and augments their activation. J. Immunol. 190:4263-4273. https://doi.org/10.4049/jimmunol.1200732
  12. Kimchi-Sarfaty, C., J. M. Oh, I. W. Kim, Z. E. Sauna, A. M. Calcagno, S. V. Ambudkar, and M. M. Gottesman. 2007. A "silent" polymorphism in the MDR1 gene changes substrate specificity. Science 315(5811):525-528. https://doi.org/10.1126/science.1135308
  13. Liu, Y., X. T. Qiu, J. J. Xu, F. Hu, Y. H. Li, H. J. Li, Y. F. Gong, and Q. Zhang. 2011. Association analysis between the polymorphism of the SLC11A1 gene and immune response traits in pigs. Asian J. Anim. Vet. Adv. 6:580-586. https://doi.org/10.3923/ajava.2011.580.586
  14. Meilang, Q., Y. Zhang, J. Zhang, Y. Zhao, C. Tian, J. Huang, and H. Fan. 2012. Polymorphisms in the SLC11A1 gene and tuberculosis risk: a meta-analysis update. Int. J. Tuberc. Lung Dis. 16:437-446. https://doi.org/10.5588/ijtld.10.0743
  15. Pauly, T., K. Elbers, M. Konig, T. Lengsfeld, A. Saalmuller, and H. J. Thiel. 1995. Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope. J. Gen. Virol. 76:3039-3049. https://doi.org/10.1099/0022-1317-76-12-3039
  16. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York, USA.
  17. Sapkota, B. R., M. Hijikata, I. Matsushita, G. Tanaka, R. Ieki, N. Kobayashi, E. Toyota, H. Nagai, A. Kurashima, K. Tokunaga, and N. Keicho. 2012. Association of SLC11A1 (NRAMP1) polymorphisms with pulmonary Mycobacterium avium complex infection. Hum. Immunol. 73:529-536. https://doi.org/10.1016/j.humimm.2012.02.008
  18. Smit, J. J., G. Folkerts, and F. P. Nijkamp. 2004. Ramp-ing up allergies: Nramp1 (Slc11a1), macrophages and the hygiene hypothesis. Trends Immunol. 25:342-347. https://doi.org/10.1016/j.it.2004.04.010
  19. Tanaka, G., J. Shojima, I. Matsushita, H. Nagai, A. Kurashima, K. Nakata, E. Toyota, N. Kobayashi, K. Kudo, and N. Keicho. 2007. Pulmonary Mycobacterium avium complex infection: Association with NRAMP1 polymorphisms. Eur. Respir. J. 30: 90-96. https://doi.org/10.1183/09031936.00042506
  20. Tuggle, C. K., C. B. Schmitz, and D. Gingerich-Feil. 1997. Rapid communication: cloning of a pig full-length NRAMP1 cDNA. J. Anim. Sci. 75:277.
  21. Tuggle, C. K., L. Marklund, T. J. Stabel, M. A. Mellencamp, and A. Stumbaugh. 2005. Genetic markers for screening animals for improved disease resistance (NRAMP). United States Patent, Patent No. :US 6,844,159 B2.
  22. Vidal, S. M., D. Malo, K. Vogan, E. Skamene, and P. Gros. 1993. Natural resistance to infection with intracellular parasites: isolation of a candidate for Bcg. Cell 73:469-485. https://doi.org/10.1016/0092-8674(93)90135-D
  23. Wu, H., D. Cheng, and L. Wang. 2008. Association of polymorphisms of Nramp1 gene with immune function and production performance of Large White pig. J. Genet. Genomics 35:91-95. https://doi.org/10.1016/S1673-8527(08)60014-4
  24. Wyllie, S., P. Seu, and J. A. Goss. 2002. The natural resistanceassociated macrophage protein 1 NRAMP1 (formerly Nramp1) and iron metabolism in macrophages. Microbes Infect. 4:351-359. https://doi.org/10.1016/S1286-4579(02)01548-4
  25. Yang, J. H., K. Downes, J. M. Howson, S. Nutland, H. E. Stevens, N. M. Walker, and J. A. Todd. 2011. Evidence of association with type 1 diabetes in the SLC11A1 gene region. BMC Med. Genet. 12:59.

Cited by

  1. Transcriptome changes in muscle of Nellore cows submitted to recovery weight gain under grazing condition pp.1751-732X, 2018, https://doi.org/10.1017/S1751731118001490
  2. Sequencing and phylogenetic analysis of the SLC11A1 gene in pigs vol.45, pp.1, 2017, https://doi.org/10.1080/09712119.2016.1218885
  3. Sequencing and phylogenetic analysis of the SLC11A1 gene in pigs vol.45, pp.1, 2017, https://doi.org/10.1080/09712119.2016.1218885
  4. Genomic analysis reveals selection signatures of the Wannan Black pig during domestication and breeding vol.33, pp.5, 2014, https://doi.org/10.5713/ajas.19.0289
  5. Genome-wide scan for runs of homozygosity identifies candidate genes in Wannan Black pigs vol.34, pp.12, 2014, https://doi.org/10.5713/ab.20.0679