Journal of the Korean Magnetic Resonance Society (한국자기공명학회논문지)

Korean Magnetic Resonance Society (한국자기공명학회)
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NMR Studies on Transient Protein Complexes: Perspectives

  • Suh, Jeong-Yong (Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University) ;
  • Yu, Tae-Kyung (Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University) ;
  • Yun, Young-Joo (Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Ko On (Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University)
  • Received : 2014.05.01
  • Accepted : 2014.06.10
  • Published : 2014.06.20
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Abstract

It is generally understood that protein-protein interactions proceed via transient encounter complexes that rapidly evolve into the functional stereospecific complex. Direct detection and characterization of the encounter complexes, however, been difficult due to their low population and short lifetimes. Recent application of NMR paramagnetic relaxation enhancement first visualized the structures of the encounter complex ensemble, and allowed the characterization of their physicochemical properties. Further, rational protein mutations that perturbed the encounter complex formation provided a clue to the target search pathway during protein-protein association. Understanding the structure and dynamics of encounter complexes will provide useful information on the mechanism of protein association.

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References

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