Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant and Antihemolytic Activity of Ethanol Extracts of Rubus coreanus Miquel

복분자 에탄올 추출물의 항산화 및 항용혈 효능

  • Jang, Tae-Su (Institute of Green Bio Science & Technology Seoul National University) ;
  • Yang, Jae-Chan (College of Sciences & Technology, Division of Biomedicinal & Cosmetics, Mokwon University) ;
  • Lim, Sun-Young (Institute of Green Bio Science & Technology Seoul National University) ;
  • Kim, Bo-Ae (College of Sciences & Technology, Division of Biomedicinal & Cosmetics, Mokwon University)
  • 장태수 (서울대학교 그린바이오과학기술연구원) ;
  • 양재찬 (목원대학교 테크노과학대학 생의약화장품학부) ;
  • 임선영 (서울대학교 그린바이오과학기술연구원) ;
  • 김보애 (목원대학교 테크노과학대학 생의약화장품학부)
  • Received : 2014.02.27
  • Accepted : 2014.04.03
  • Published : 2014.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

To develop a natural antioxidant and anti-hemolytic agents, we investigated the effects of ethanol extracts of Rubus coreanus Miquel (crude extracts). Crude extract was extracted with ethanol. Antioxidant activity of crude extracts was evaluated by employing two different assays, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity and super oxide dismutase(SOD)-like activity. Also, anti-hemolytic activity of crude extract was determined using $H_2O_2$-induced hemolysis in normal rat red blood cells (RBC) or plasma. The extracts obtained from crude extract dose-dependently increased the scavenging activity on DPPH-induced radicals and SOD-like activity. RBC oxidative hemolysis induced by $H_2O_2$ was significantly suppressed by the extracts of Rubus coreanus Miquel in a dose-dependent manner. These results suggest that crude extracts may have value as the potential antioxidant and anti-hemolytic medicinal plant.

천연에서 유래한 항산화 및 항용혈 소재개발을 위해 복분자 에탄올 추출물의 생리활성 효능 평가를 실시하였다. 복분자를 에탄올 추출한 후, 획득한 추출물을 섬유아세포주인 HS68에 처리하여 DPPH 라디칼 소거능과 SOD 유사 항산화 효능을 평가하였다. 또한 Sprague Dawley 렛트 적혈구 세포에 과산화수소로 산화를 유도한 후 추출물의 항용혈 효능을 살펴보았다. 그 결과 추출물은 농도 의존적으로 DPPH 라디칼 소거능과 SOD 유사 활성이 증가하는 것으로 나타났다. 렛트 적혈구를 이용한 항용혈 평가에서는 과산화수소를 단독으로 처리한 양성대조군보다 추출물을 처리한 군에서 항용혈율이 농도 의존적으로 유의하게 증가하였다. 이들 결과는 복분자 추출물이 항산화효능과 항용혈 약용 식물 소재로서의 가능성을 내는 것으로 사료된다.

Keywords

References

  1. W. G. Cho, Comparision of Drug Delivery using Hairless and Pig Skin. J. of Korea Oil Chemists'Soc., 24(4), 410 (2007).
  2. I. C. Kim, Antioxidative Property and Whitening Effect of the Polygoni Multiflori Radix, Polygonati Rhizoma and Ephedrae Herba. J. of Korea Oil Chemists' Soc., 25(4), 533 (2008).
  3. K. C. Sung, A study on the Antimicrobial Effect of Natural Artemisia Extract using Super Critical Cabon Dioxide. J. of Korea Oil Chemists' Soc., 20(4), 309 (2004).
  4. H. Wiseman, Dietary influences on membrane function; impotent in protection against oxidative damage and disease. Nutr Biochem., 7(1), 2 (1996). https://doi.org/10.1016/0955-2863(95)00152-2
  5. J. Bouayed, T. Bohn, Exogenous antioxidants-Double-edged swords in cellular redox state: Health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxid Med Cell Longev., 3(4), 228 (2010). https://doi.org/10.4161/oxim.3.4.12858
  6. S. Kawashima, The possible role of lipoperoxide in aging. Nagoya J. Med Sci., 32, 303 (1970).
  7. E. A. Dicker, A. D. Crum, J. T. Calvert, Differences in the antioxidant mechanism of carmosine in the prescence of copper and iron. J, Agric Food Chem., 40(5), 756 (1992). https://doi.org/10.1021/jf00017a009
  8. B. H. Halliwell, J. M. C. Gutteridge, Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol., 186, 1 (1990). https://doi.org/10.1016/0076-6879(90)86093-B
  9. S. M. Edgington, As we live and breathe: free radicals and aging. Correlative evidence from a number of fields suggests they may be key. Bio Technology, 12(1), 37 (1994). https://doi.org/10.1038/nbt0194-37
  10. J. L. Vives Corrons, A. Miguel-Garcia, M. A. Pujades, A. Miguel-Sosa, S. Cambiazzo, M. Linares, M. T. Dibarrart, M. A. Calvo, Increased susceptibility of microcytic red blood cells to in vitro oxidative stress. Eur J. Haematol., 55(5), 327 (1995).
  11. C. Rice-Evans, S. C. Omorphos, E. Baysal, Sickle cell membranes and oxidative damage. Biochem J. 237(1), 265 (1986).
  12. S. M. Sadrzadeh, E. Graf, S. S. Panter, P. E. Hallaway, J. W. Eaton, Hemoglobin. A biologic fenton reagent. J Biol Chem., 259(23), 14354 (1984).
  13. M. R. Clemens, M. Ruess, Z. Bursa, H. D. Waller, The relationship between lipid composition of red blood cells and their susceptibility to lipid peroxidation. Free Radical Res Commun., 3(1-5), 265 (1987). https://doi.org/10.3109/10715768709069792
  14. M. S. Kim, G. C. Pang, M. W. Lee, Flavonoids from the leaves of Rubus coreanum. J. Pharm Soc Korea, 41, 1 (1997).
  15. J. Heo, Donguibogam 1-5. Yeogang Publishing Co., Seoul, Korea, 62 (1994).
  16. Pang KC, Kim MS, Lee MW (1996) Hydrolyzable tannins from the fruits of Rubus coreanum. Korean J. Pharm., 27, 366 (1996).
  17. Y. A. Lee, M. W. Lee, Tannins from Rebus coreaum. Korean J. Pharm., 26, 27 (1995).
  18. M. W. Lee, Phenolic compounds from the leaves of Rubus coreaum. J. Pharm Soc Korea, 39, 200 (1995).
  19. Kim JH, Kim CH, Kim HS, Kwon MC, Song YK, Seong NS, Lee SE, Yi JS, Kwon OW, Lee HY, Effect of aqueous extracts from Rubus coreanus Miquel and Angelica gigas Nakai on anti-tumor and anti-stress activities in mice. Korean J. Med Crop Sci., 14(4), 206 (2006).
  20. M. K. Lee, H. S. Lee, G. P. Choi, D. H. Oh, J. D. Kim, C. Y. Yu, H. Y. Lee, Screening of biological activities of the extracts from Rubus coreanus Miq. Korean J. Med Crop Sci., 11(1), 5 (2003).
  21. K. H. Sung, J. H. Lee, A study on quality characteristics of Teriyaki sauce with added Rubus coreanus Miquel. J. East Asian Soc. Diet Life, 19(6), 958 (2009).
  22. Y. J. Song, A study to marketing strategies for the korean wild-berry wine industry. Korean Bus. Edu. Assoc., 4, 119 (2004).
  23. S. M. Hong, M. J. Kang, J. H. Lee, J. H. Jeong, S. H. Kwon, K. I. S, Production of Vinegar using Rubus coreanus and Its Antioxidant Activities. Korean J. Food Preserv., 19(4), 594 (2012). https://doi.org/10.11002/kjfp.2012.19.4.594
  24. M. S. Blois, Antioxidant determinations by the use of a stable free radical. Nature 26, 1199 (1958).
  25. C. K. Sen, Oxidative and antioxidants in exercise. J. Appl Physiol. 79(3), 675 (1995).
  26. M. A. Ebrahimzadeh, S. F. Nabavi, S. M. Nabavi, B. Eslami, Antihemolytic and antioxidant activities of Allium paradoxum. Central European J. Biology. 5(3), 338 (2010).

Cited by

  1. Nutrition Composition and Single, 14-Day and 13-Week Repeated Oral Dose Toxicity Studies of the Leaves and Stems of Rubus coreanus Miquel vol.21, pp.1, 2016, https://doi.org/10.3390/molecules21010065
  2. Effects of Rubus coreanus byproducts on intestinal microbiota and the immune modulation vol.31, pp.3, 2018, https://doi.org/10.5713/ajas.17.0733
  3. 복분자(Rubus coreanus) 추출물의 라디칼 소거활성 및 고지방 식이에 대한 흰쥐의 간 지질 개선 효과 vol.38, pp.2, 2014, https://doi.org/10.7853/kjvs.2015.38.2.117
  4. 신선육자환 에탄올 추출물의 항산화 활성 vol.32, pp.5, 2017, https://doi.org/10.6116/kjh.2017.32.5.19