Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification and Characterization of ${\alpha}$-Neoagarooligosaccharide Hydrolase from Cellvibrio sp. OA-2007

  • Ariga, Osamu (School of Environmental Science and Engineering, Kochi University of Technology) ;
  • Okamoto, Naoki (School of Environmental Science and Engineering, Kochi University of Technology) ;
  • Harimoto, Naomi (School of Environmental Science and Engineering, Kochi University of Technology) ;
  • Nakasaki, Kiyohiko (International Development Engineering, Graduate School of Engineering, Tokyo Institute of Technology)
  • Received : 2013.07.10
  • Accepted : 2013.09.27
  • Published : 2014.01.28
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Abstract

${\alpha}$-Neoagarooligosaccharide (${\alpha}$-NAOS) hydrolase was purified from Cellvibrio sp. OA-2007 by using chromatographic techniques after hydroxyapatite adsorption. The molecular masses of ${\alpha}$-NAOS hydrolase estimated using SDS-PAGE and gel filtration chromatography were 40 and 93 kDa, respectively, and the optimal temperature and pH for the enzyme activity were $32^{\circ}C$ and 7.0-7.2. ${\alpha}$-NAOS hydrolase lost 43% of its original activity when incubated at $35^{\circ}C$ for 30 min. The enzyme hydrolyzed neoagarobiose, neoagarotetraose, and neoagarohexaose to galactose, agarotriose, and agaropentaose, respectively, and produced 3,6-anhydro-L-galactose concomitantly; however, it did not degrade agarose.

Keywords

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