Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of a Novel Immunochromatographic Assay for Rapid Detection of VanA Ligase-Producing Vancomycin-Resistant Enterococci

  • Ji, Gil Yong (Department of Pathology Medicine, Chungbuk National University College of Medicine) ;
  • Song, Hyung Geun (Department of Pathology Medicine, Chungbuk National University College of Medicine) ;
  • Son, Bo Ra (Department of Laboratory Medicine, Chungbuk National University College of Medicine) ;
  • Hong, Seung Bok (Department of Clinical Laboratory Science, Chungbuk Health and Science University) ;
  • Kim, Jong Wan (Department of Laboratory Medicine, Dankook University College of Medicine) ;
  • Shin, Kyeong Seob (Department of Laboratory Medicine, Chungbuk National University College of Medicine)
  • Received : 2013.07.11
  • Accepted : 2013.12.11
  • Published : 2014.03.28
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Abstract

We developed a novel immunochromatographic assay (ICA) (EZ-Step VanA rapid kit; Dinona, Korea) for the detection of VanA ligase from vancomycin-resistant enterococci (VRE). Of eight monoclonal antibodies screened by ELISAs, the VanA ligase ICA constructed with 1H9 plus 3G11 showed the greatest reactivity. The detection limit of the kit was $6.3{\times}10^6$ CFU per test. Of 127 vancomycin-resistant microorganisms, 100 vanA VRE were positive in the VanA ligase ICA, and 27 non-vanA vancomycin-resistant isolates were negative. These results were consistent with those of the PCR analyses. Thus, our ICA is a reliable and easy-to-use immunological assay for detecting VanA-producing VRE in clinical laboratories.

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