Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Antineoplastic Effect of Several Herbal Medicines on SNU-80 Anaplastic Thyroid Carcinoma Cell Line

황기, 당귀, 칠피, 천화분의 역형성갑상선암세포 SNU-80에 대한 항암효과

  • Yeo, Hyun Soo (Department of Korean Internal Medicine, College of Korean Medicine, Gachon University) ;
  • Lee, Min Hye (Department of Korean Internal Medicine, College of Korean Medicine, Gachon University) ;
  • Ko, Seong Gyu (Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University) ;
  • Choi, You Kyung (Department of Korean Internal Medicine, College of Korean Medicine, Gachon University) ;
  • Jun, Chan Young (Department of Korean Internal Medicine, College of Korean Medicine, Gachon University) ;
  • Park, Jong Hyeong (Department of Korean Internal Medicine, College of Korean Medicine, Gachon University)
  • 여현수 (가천대학교 한의과대학 내과학교실) ;
  • 이민혜 (가천대학교 한의과대학 내과학교실) ;
  • 고성규 (경희대학교 한의과대학 예방의학교실) ;
  • 최유경 (가천대학교 한의과대학 내과학교실) ;
  • 전찬용 (가천대학교 한의과대학 내과학교실) ;
  • 박종형 (가천대학교 한의과대학 내과학교실)
  • Received : 2014.03.31
  • Accepted : 2014.04.12
  • Published : 2014.04.30
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Abstract

Objective : This study was performed to investigate the antineoplastic effect of Astragalus membranaceus, Angelica gigas, Rhus verniciflua Stokes and Trichosanthes kirilowii on SNU-80 anaplastic thyroid carcinoma cell line. Method : We examined whether our herbal medicines decreases cell growth rate of SNU-80 using MTT assay. We performed western blot analysis to verify that our herbal medicines induces apoptosis via caspase-dependent mechanism. We also performed wound healing assay and transwell invasion assay to investigate whether our herbal medicines affects the migration and invasion of anaplastic cancer cells, SNU-80. We also carried out ELISA assay to know our herbal medicines suppresses the expression of proinvasive molecules, such as VEGF and MMP-2 secreted from SNU-80. Results : MTT assay demonstrates that Angelica gigas, Rhus verniciflua Stokes, and Trichosanthes kirilowii suppressed strongly the growth of SNU-80. Western blot analysis demonstrates that Trichosanthes kirilowii induces apoptosis activating the cleavages of caspases (caspase-8, caspase-3) and PARP. Wound healing assay demonstrates that Rhus verniciflua Stokes, and Trichosanthes kirilowii inhibited the migration of SNU-80. Transwell invasion assay demonstrates that Rhus verniciflua Stokes, and Trichosanthes kirilowii inhibited the invasion of SNU-80. Elisa assay demonstrates that Astragalus membranaceus, Angelica gigas, Rhus verniciflua Stokes, and Trichosanthes kirilowii suppressed the expression of VEGF and MMP-2. Conclusion : We could conclude that several herbal medicines suppresses the growth and inhibits the migration and invasion of SNU-80 which is anaplastic thyroid cancer cells. Especially, Rhus verniciflua Stokes, Trichosanthes kirilowii had stronger anti-cancer effect suggesting that we can apply them to treat anaplastic thyroid cancer.

Keywords

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