Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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The Polyphenol Chlorogenic Acid Attenuates UVB-mediated Oxidative Stress in Human HaCaT Keratinocytes

  • Cha, Ji Won (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Piao, Mei Jing (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Kim, Ki Cheon (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Yao, Cheng Wen (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Zheng, Jian (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Kim, Seong Min (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Hyun, Chang Lim (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Ahn, Yong Seok (Research Institute of Processing from Jeju Fisher Food, Choung Ryong Fisheries Co., LTD.) ;
  • Hyun, Jin Won (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University)
  • Received : 2014.01.24
  • Accepted : 2014.03.06
  • Published : 2014.03.31
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Abstract

We investigated the protective effects of chlorogenic acid (CGA), a polyphenol compound, on oxidative damage induced by UVB exposure on human HaCaT cells. In a cell-free system, CGA scavenged 1,1-diphenyl-2-picrylhydrazyl radicals, superoxide anions, hydroxyl radicals, and intracellular reactive oxygen species (ROS) generated by hydrogen peroxide and ultraviolet B (UVB). Furthermore, CGA absorbed electromagnetic radiation in the UVB range (280-320 nm). UVB exposure resulted in damage to cellular DNA, as demonstrated in a comet assay; pre-treatment of cells with CGA prior to UVB irradiation prevented DNA damage and increased cell viability. Furthermore, CGA pre-treatment prevented or ameliorated apoptosis-related changes in UVB-exposed cells, including the formation of apoptotic bodies, disruption of mitochondrial membrane potential, and alterations in the levels of the apoptosis-related proteins Bcl-2, Bax, and caspase-3. Our findings suggest that CGA protects cells from oxidative stress induced by UVB radiation.

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References

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