Journal of Korean Society of Environmental Engineers (대한환경공학회지)

Korean Society of Environmental Engineers (대한환경공학회)
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Nitrogen Removals according to Aeration/Non-aeration Periods in the Intermittent Aeration Reactor and Analysis of Microbial Community

간헐포기공정에서 포기/비포기 구간에 따른 질소제거 및 미생물 군집분석

  • Choi, Moon-Su (Department of Environmental Engineering, Seoul National University of Science & Technology) ;
  • Lee, Tae-Jin (Department of Environmental Engineering, Seoul National University of Science & Technology)
  • 최문수 (서울과학기술대학교 환경공학과.폐자원바이오매스 에너지화 센터) ;
  • 이태진 (서울과학기술대학교 환경공학과.폐자원바이오매스 에너지화 센터)
  • Received : 2013.12.09
  • Accepted : 2013.12.31
  • Published : 2014.01.31
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Abstract

In this study, variations of the organic and nitrogenous compounds in wastewater were investigated in a single reactor with intermittent aeration. Over 90% of organic and nitrogen removals are accomplished with C/N ratio of 3 : 1 and 20/20 min of aeration/non-aeration period. Longer non-aeration period on the aeration/non-aeration cycle showed more stable nitrogen removal, showing various microbial community in the reactor. From PCR-DGGE analysis, it is conclusive that Dysgonomonas mossii strain Melo40, Eubacterium sp. oral clone JN088, Uncultured bacterium clone SPESB2_718, and Bacterium enrichment culture clone LE are related with the organics and nitrogen oxidation. Uncultured Acidobacteria bacterium clone AKYG487, Lactobacillus harbinensis strain FQ003, Erythrobacter litoralis strain Gi-3, Phytobacter diazotrophicus strain Ls8, and Mycobacterium sp. enrichment culture clone GE10037biofNNA are distinctly appeared under denitrification condition.

본 연구에서는 단일반응조에서 포기/비포기 시간에 따른 하수 내 유기물질 및 질소화합물을 변화양상을 살펴보고자 하였다. 실험 결과 C/N비 3 : 1, 포기/비포기 20/40 min 구간에서부터 90% 이상의 안정적인 유기물 및 질소 제거가 이루어짐을 알 수 있었다. 포기/비포기 구간의 비율을 길게 유지하는 것이 탈질에 더욱 효과적이었으며 이는 비포기 구간을 유지하는 동안 반응조 내 미생물의 군집변화에 기인하는 것으로 판단하였다. PCR-DGGE를 한 결과, 유기물 및 질소화합물의 산화에 관여하는 미생물로 Dysgonomonas mossii strain Melo40, Eubacterium sp. oral clone JN088, Uncultured bacterium clone SPESB2_718과 Bacterium enrichment culture clone LE이 관찰되었고 탈질에 관여하는 미생물은 Uncultured Acidobacteria bacterium clone AKYG487, Lactobacillus harbinensis strain FQ003, Erythrobacter litoralis strain Gi-3, Phytobacter diazotrophicus strain Ls8, Mycobacterium sp. enrichment culture clone GE10037biofNNA로 나타났다.

Keywords

References

  1. Park, K. H. and Kwon, Y. D., "A Study on the Characteristics of Process for the Sewage Treatment in Korea," J. Kor. Soc. Environ. Anal., 15(1), 9-14(2012).
  2. Lim, J.-W., Lim, P.-E. and Seng, C.-E., "Enhancement of nitrogen removal in moving bed sequencing batch reactor with intermittent aeration during REACT period," Chem. Eng. J., 197, 199-203(2012). https://doi.org/10.1016/j.cej.2012.05.036
  3. Moura, R. B., Damianovic, M. H. and Foresti, E., "Nitrogen and carbon removal from synthetic wastewater in a vertical structured-bed reactor under intermittent aeration," J. Environ. Manage., 98, 163-167(2012). https://doi.org/10.1016/j.jenvman.2012.01.009
  4. Fang, J. and Barcelona, M. J., "Biogeochemical evidence for microbial community change in a get fuel hydrocarbons contaminated aquifer," Org. Geochem., 29(4), 899-907(1997).
  5. Hedrick, D. B. and White, D. C., "Microbial respiratory quinones in the environment 1. A sensitive liquid chromatographic method," J. Microbiol. Methods, 5, 243-254(1986). https://doi.org/10.1016/0167-7012(86)90049-7
  6. Hiraishi, A., "Respiratory quinone profiles as tool for identifying different bacterial populations in activated sludge," J. Gen. Appl. Microbiol., 34, 39-56(1988). https://doi.org/10.2323/jgam.34.39
  7. Fujie, K., Hu, H.-Y., Tanaka, H. and Urano, K., "Ecological studies of aerobic submerged biofilter on the basis of resporatory quinone profiles," Water Sci. Technol., 29, 373-376(1994).
  8. Hu, H.-Y., Fujie, K., Tanaka, H., Makabe, T. and Urano, K., "Respiratory qyinone profile as a tool for refractory chemical biodegradation study," Water Sci. Technol., 35, 103-110(1997).
  9. Hess, A., Hollander, R. and Mannheim, W., "Lipoquinones of some spore-forming rods, lactic-acid bacteria and actinomycetes," J. Gen. Microbiol., 115, 247-252(1979). https://doi.org/10.1099/00221287-115-1-247
  10. Amann, R, I., Ludwig, W. and Schleifer, K.-H., "Phylogenetic identification and insitu detection of individual microbial cells without cultivation," Microbiol. Rev., 59(1), 143-169(1995).
  11. Vreas, L., Forney, L., Daae, F. L. and Torsvik, V., "Distribution of Bacterioplankton in meromictic lake selenvannet, as determined by denaturing gradient gel electrophoresis of PCRAmplified gene fragments coding for 16S rRNA," Appl. Environ. Microbiol., 63(9), 3367-3373(1997).
  12. Hollibaugh, J. T., Bano, N. and Ducklow, H., "Widespread distribution in polar oceans of a 16S rRNA gene sequence with affinity to Nitrosospira-like ammonia-oxidizing bacteria," Appl. Environ. Microbiol., 68(3), 1478-1484(2002). https://doi.org/10.1128/AEM.68.3.1478-1484.2002
  13. Pedro, M. S., Haruta, S., Hazaka, M., Shimada, R., Yoshida, C., Hiura, K., Ishii, M. and Igarashi, Y., "Denaturing gradient gel electrophoresis analysis of microbial community from field-scale composter," J. Biosci. Bioeng., 91(2), 159-165 (2001). https://doi.org/10.1016/S1389-1723(01)80059-1
  14. Ministry of Environment, Standard Methods for examination of water and wastewater(2004).
  15. APWA, AWWA, WPCF, Standard methods for the examination of water and wastewater, 21th ed., (2005).
  16. Arayz, R., Tani, K., Yamaguchi., N. and Nasu, M. "Bacterial activity and community composition in stream water and biofilm from an urban river determined by flourescent in situ hybridization and DGGE analysis," FEMS Microbiol. Ecol., 43, 111-119(2003). https://doi.org/10.1111/j.1574-6941.2003.tb01050.x
  17. Vreas, L., Forney, L., Daae, F. L. and Torsvik, V., "Distribution of bacterioplankton in meromictic lake selenvannet, as determined by denaturing gradient gel electrophoresis of PCR-Amplified gene fragments coding for 16S rRNA," Appl. Environ. Microbiol., 63(9), 3367-3373(1997).
  18. Pedro, M. S., Haruta, S, Hazaka, M., Shimada, R., Yoshida, C., Hiura, K., Ishii, M. and Igarashi, Y., "Denaturing gradient gel electrophoresis analyses of microbial community from field-scale composter," J. Biosci. Bioeng., 91(2), 159-165 (2001). https://doi.org/10.1016/S1389-1723(01)80059-1
  19. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F., "Colorimetric method for determination of sugars and related substances," Anal. Chem., 28(3), 350-356(1956). https://doi.org/10.1021/ac60111a017
  20. Minnan, L., Jinli, H., Xuijuan, W., Jinzao, C., Chuannan, L., Fengzhang, Z. and Liangshu, X., "Isolation and characterization of a high H2-Producing strain Klebsiella oxytoca HP1 from a hot spring," Res. Microbiol., 156, 76-81(2005). https://doi.org/10.1016/j.resmic.2004.08.004
  21. Hung, C. H., Cheng, C. H., Cheng, L. H., Liang, C. M. and Lin, C. Y., "Application of Clrostridium-specific PCR primers on the analysis of dark fermentation hydrogen-producing bacterial community," Inter. J. Hydrogen Energy(2007).
  22. Auch, A. F., Henz, S. R., Holland, B. R. and Goker, M., "Genome BLAST distance phylogenies inferred from whole plastid and whole mitochondrion genome sequences," Bio- Med Central Ltd, (2006).
  23. Felgenstein, J., "Confidence limits on phylogenetics: an approach using the bootstrap," Evolution, 39, 783-791(1985). https://doi.org/10.2307/2408678
  24. Kimura, M. "A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences," J. Mol. Evol., 16, 111-120(1980). https://doi.org/10.1007/BF01731581
  25. Saitou, N. and Nei, M., "The neighbour-joining method: a new method for constructing phylogenetic trees," Mol. Biol. Evol., 4, 406-425(1987).
  26. Costilow, R. N. and Humphreyts, W., "Nitrate reduction by certain strains of Lactobacillus plantaru," Science, 121, 168 (1955). https://doi.org/10.1126/science.121.3136.168
  27. Clarker, T. J., "A dextran-fermenting organism from the lumen closely resembling Lactobacillus biBdus," J. Gen. Microbiol, 20, 549(1959). https://doi.org/10.1099/00221287-20-3-549
  28. Rogosa, M., "Experimental conditions for Nitrate reduction by certain strains of the genus Lactobacillus," J. Gen. Microbiol, 24, 401-408(1961). https://doi.org/10.1099/00221287-24-3-401
  29. Shioi, Y., Doi, M., Arata, H. and Takamiya., K., "A Denitrifying Activity in an Aerobic Photosynthetic Bacterium, Erythrobacter sp. Strain OCh 114," Plant Cell Physiol., 29(5), 861-865(1988).
  30. Fritz, C., Maass, S., Kreft, A. and Bange., F.-C. "Dependence of Mycobacterium bovis BCG on anaerobic nitrate reductase for persistence is tissue specific," Infect. Immun, 70, 286-291(2002). https://doi.org/10.1128/IAI.70.1.286-291.2002
  31. Voskuil, M. I., Schnappinger, D., Harrell, M. I., Visconti, K. C., Dolganov, G. M., Sherman, D. R. and Schoolnik, G. K., "Inhibition of respiration by nitric oxide induces a Mycobacterium tuberculosis persistence program," J. Exp. Med, 198, 705-713(2002).
  32. Choi, C. K., Han, J. H. and Lee, K. H., "Evaluation of aeration on/off time, C/N ratio and ORP applicability for nutrient removal in intermittent-aeration membrane bioreactor (IAMBR)," J. Kor. Soc. Environ. Eng., 156-162(2003).
  33. Lim, B. S. Choi, B. C. Yu, S.W. and Lee, C. G. "Effects of operational parameters on aeration on/off time in an intermittent aeration membrane bioreactor," Desalination, 202, 77-82(2007). https://doi.org/10.1016/j.desal.2005.12.041
  34. Choi, B. C., Kim, K. S., Yang, Y. H. and Lim, B. S., "Evaluation of aeration on/off time optimum in intermittent aeration membrane bioreactor for advanced wastewater treatment," In Proceedings of The Korean Society of Water and Wastewater and the Korean society of Water Environment 2004 Joint conference, pp. 736-743(2004).
  35. Choi, C. K., Lee, J. K., Lee, K. H. and Kim, M. I., "The effects on operation conditions of sludge retention time and carbon/nitrogen ratio in an intermittently aerated membrane bioreactor (IAMBR)," Bioresour. Technol., 99, 5397-5401 (2008). https://doi.org/10.1016/j.biortech.2007.11.016