The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Study on Development Effect on Zebrafish Embryo by Alacholr, Butachlor and Fipronil

농약 alacholr, butachlor 및 fipronil이 제브라피쉬 배아 발생에 미치는 영향

  • Park, Soo Jin (Korea Bio-Safety Institute Co., LTD.) ;
  • Jeong, Mihye (Agro-Material Safety Evaluating Division, Department of Agro-Food Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Paik, Min-Kyoung (Chemical Safety Division, Department of Agro-Food Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Je-Bong (Agro-Material Safety Evaluating Division, Department of Agro-Food Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • You, Are-Sun (Agro-Material Safety Evaluating Division, Department of Agro-Food Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Hong, SoonSung (Agro-Material Safety Evaluating Division, Department of Agro-Food Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ihm, Yang Bin (Agro-Material Safety Evaluating Division, Department of Agro-Food Safety, National Academy of Agricultural Science, Rural Development Administration)
  • 박수진 ((주)한국생물안전성연구소) ;
  • 정미혜 (농촌진흥청 국립농업과학원 농산물안전성부 농자재평가과) ;
  • 백민경 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 이제봉 (농촌진흥청 국립농업과학원 농산물안전성부 농자재평가과) ;
  • 유아선 (농촌진흥청 국립농업과학원 농산물안전성부 농자재평가과) ;
  • 홍순성 (농촌진흥청 국립농업과학원 농산물안전성부 농자재평가과) ;
  • 임양빈 (농촌진흥청 국립농업과학원 농산물안전성부 농자재평가과)
  • Received : 2013.11.14
  • Accepted : 2014.02.05
  • Published : 2014.03.31
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Abstract

This study is aimed to search the possibility of developmental toxicity test using the zebrafish from the pesticide. We selected herbicides alachlor and butachlor, reported for fish toxicity, and insecticide fipronil reported for the high fish toxicity and the honey bee risk among the pesticides with high usability for the examples of the pesticides in this experiment. In this study, we showed those effects on the zebrafish embryo development by exposing different kinds of pesticide with different concentration and exposed time periods. As a result, the rates of hatching and abnormality of the zebrafish embryo after treatments of alachlor were increased in 24-48 hpf group, and the juvenile fishes in every group exposed to $40{\mu}M$ or more of alachlor displayed sever morphological changes such as bent tails, edema and activity failures. In case of the butachlor, the rates of hatching and the abnormality in 24-48 hpf group were higher than the other groups exposed in different time periods. The fatality before hatching was high in $40{\mu}M$ or more of butachlor treatment, and entire zebrafish embryos in 48 hpf group died before hatching. All the living juvenile fishes showed morphological changes as like as the treatment of alachlor. The rate of hatching and the survival of the zebrafish embryo by the fipronil were higher than other pesticides. However, morphological changes such as bent tails were observed from the most of living juvenile fishes. Therefore, the effects of three different pesticides with different concentrations and exposing time periods on the development of zebrafish embryos showed that all the pesticides effects were proportional to the concentration, and exposing time periods may cause the morphological abnormality.

본 연구에서는 농약에서 제브라피쉬를 이용한 발생독성시험 가능성을 탐색하고자 사용양이 많은 농약 중 어독성이 보고된 제초제 alachlor 및 butachlor와 어독성이 높고 꿀벌위해성 등이 보고된 살충제 fipronil을 시험물질로 선정하였고, 농도와 노출시기를 달리하여 제브라피쉬 배아에 노출시켜 발생에 미치는 영향을 측정하였다. 그 결과, alachlor에 의한 제브라피쉬 배아의 부화율 및 이상율은 24-48 hpf 군이 높았고, $40{\mu}M$ 이상 농도에 노출된 모든 군의 치어에서 꼬리휨, 부종 및 활동성 저하의 증상이 관찰되었다. Butachlor의 경우 24-48 hpf 군의 부화율과 이상율이 다른 시기에 노출된 군에 비해 높았고, $40{\mu}M$ 이상 농도에서 부화전 치사율이 높았으며, 48 hpf 군 제브라피쉬 배아는 부화 전 모두 치사되었다. 생존한 모든 치어에서 꼬리휨, 부종 등 증상이 관찰되었다. Fipronil에 의한 제브라피쉬 배아의 부화율 및 생존율은 다른 농약에 비해 높았으나 생존한 대부분의 치어에서 꼬리휨 증상이 관찰되었다. 따라서, 농약 3종을 농도와 시기를 달리하여 제브라피쉬 배아의 발생에 미치는 영향을 측정한 결과, 모든 농약이 발생에 미치는 영향은 농도에 비례하였고, 형태이상 및 이상증상은 농도 뿐 아니라, 노출시기에 따라 다르게 나타났다.

Keywords

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