Journal of Forest and Environmental Science

Institute of Forest Science, kangwon National University (강원대학교 산림과학연구소)
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In vitro Propagation and Protocorm-like Body Formation of Endangered Species, Dendrobium moniliforme

멸종위기 수종 석곡(Dendrobium moniliforme)의 Protocorm-like Body 형성 및 기내증식

  • Bae, Kee Hwa (Hongcheon Institute of Medicinal Herb) ;
  • Kim, Nam Young (Department of Horticulture and Education, Korea National Arboretum) ;
  • Song, Jae Mo (Nature Environment Research Office of Gangwon Province) ;
  • Song, Gwanpil (Jeju Environment Research Institute, Jeju Tourism College)
  • Received : 2013.05.21
  • Accepted : 2014.01.29
  • Published : 2014.02.28
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Abstract

This experiment was conducted to establish the in vitro propagation of Dendrobium moniliforme through protocorm like body (PLB) induction from the culture of leaf, stem and root explants. Frequency of PLB formation from root explants was higher than those of leaf and stem explants. PLB's proliferation in MS medium including 1.0 mg/L NAA was better than 1.0 mg/L IBA. Frequency of PLB's proliferation on medium with various concentrations of BA, Kinetin, Tidiazuron (0. 1.0, 3.0 mg/L) and NAA (0, 1.0 mg/L) was tested. The maximun proliferation of PLB's was obtained on MS medium supplemented with 3.0 mg/L BA and 1.0 mg/L NAA after 6 weeks of culture. Addition of 200 mg/L activated charcoal (AC) and 30 g/L sucrose was effective on PLB proliferation for D. moniliforme.

Keywords

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