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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Isolation and Nitrogen Removal Characteristics of Heterotrophic Nitrification-Aerobic Denitrifying Bacteria, Stenotrophomonas sp. CW-4Y

종속영양 질산화- 호기적탈질 세균 Stenotrophomonas sp. CW-4Y의 분리와 질소제거 특성

  • Lee, Eun Young (Department of Environmental and Energy Engineering, The University of Suwon) ;
  • Lee, Chang Won (Dodram Environmental Research Institute)
  • Received : 2013.12.30
  • Accepted : 2014.02.15
  • Published : 2014.02.27
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Abstract

CW-4Y was identified as Stenotrophomonas sp. by morphological and physiological characteristics, and phylogenetic analysis of its 16S rDNA gene sequence. Nitrogen removal by CW-4Y was analyzed in relation to the ammonium concentration, presence of organic carbon, carbon source, and carbon-to-nitrogen ratio (C/N). Stenotrophomonas CW-4Y has heterotrophic nitrification and aerobic denitrification abilities. Stenotrophomonas CW-4Y utilized only glucose as carbon sources, and heterotrophic nitrification and aerobic denitrification were observed regardless of the type of nitrogen source. The maximum ammonium removal rate of CW-4Y was 80 $mg-N{\cdot}L^{-1}{\cdot}d^{-1}$ and its denitrification rate of 192 $mg-N{\cdot}L^{-1}{\cdot}d^{-1}$ at $NO_3{^-}-N$ (about 280 ppm) in shake culture experiments at a C/N ratio of about 15 was about 30 times higher than those of other bacteria with the same ability.

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