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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Study on the Anti-inflammatory Effect and Mechanism of Prunus mume Extract Regarding NF-κB

NF-κB 조절을 통한 오매추출물의 항염효과 및 작용기작에 관한 연구

  • Received : 2013.12.16
  • Accepted : 2014.01.22
  • Published : 2014.02.27
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Abstract

NF-${\kappa}B$ is a transcriptional factor which is involved in many biological processes including immunity, inflammation, and cell survival. Many investigators studied on the mechanism involved in activation of NF-${\kappa}B$ signalling pathway via ubiquitination and degradation of $I{\kappa}B$ regarding skin disease. Some specific molecules including Akt, MEK, p38 MAP Kinase, Stat3, et al. represent convergence points and key regulatory proteins in signaling pathways controlling cellular events such as growth and differentiation, energy homeostasis, and the response to stress and inflammation. Ultraviolet (UV) irradiation has many adverse effects on skin, including inflammation, alteration in the extracellular matrix, cellular senescence, apoptosis and skin cancer. Prunus mume, a naturally derived plant extract, has beneficial biological activities as blood fluidity improvement, anti-fatigue action, antioxidative and free radical scavenging activities, inhibiting the motility of Helicobacter pyolri. Previous reports on various beneficial function prompted us to investigate UVB-induced or other immunostimulated biological marker regarding P. mume extract. P. mume extract suppresses UVB-induced cyclooxygenase-2 (COX-2) expression in mouse skin epidermal JB6 P+ cells. The activation of activator protein-1 and nuclear factor-${\kappa}B$ induced by UVB was dose-dependently inhibited by P. mume extract treatment. This results suggest that P. mume extracts might be used as a potential agents for protection of inflammation or UVB induced skin damage.

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References

  1. Beissert, S. and R. Granstein (1996) UV-induced cutaneous photobiology. Crit. Rev. Biochem. Mol. Biol. 31: 381-404. https://doi.org/10.3109/10409239609108723
  2. Matsumuram Y. and H. Ananthaswamy (2002) Short-term and long-term cellular and molecular events following UV irradiation of skin: implications for molecular medicine. Expert Rev. Mol. Med. 26: 1-22.
  3. Sen, R. and D. Baltimore (1988) Inducibility of kappa immunoglobulin enhancer-binding protein NF-kappa B by a posttranslational mechanism. Cell 47: 921-928.
  4. DiDonato, J. A., M. Hayakawa, D. M. Rothwarf, E. Zandi, and M. A. Karin (1997) cytokine-responsive IkB kinase that activates the transcriptional factor NF-kB. Nature 388: 548-554. https://doi.org/10.1038/41493
  5. Lin, A. and M. Karin (2003) NF-kB in cancer: a marked target. Semin. Cancer Biol. 13: 107-114.
  6. Kim, D. and J. Chung (2002) Akt: versatile mediator of cell survival and beyond. J. Biochem. Mol. Biol. 35: 106-115. https://doi.org/10.5483/BMBRep.2002.35.1.106
  7. Alessi, D. R., Y. Saito, D. G. Campbell, P. Cohen, G. Sithanandam, and U. Rapp (1994) Ashworth A, Marshall CJ, Cowley S, Identification of the sites in MAP kinase kinase-1 phosphorylated by p74 raf-1. EMBO J. 13:1610-1619.
  8. Raingeaud, J., S. Gupta, J. S. Rogers, M. Dickens, J. Han, R. J. Ulevitch, and R. J. Davis (1995) Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine. J. Biol. Chem. 270: 7420-7426. https://doi.org/10.1074/jbc.270.13.7420
  9. Zarubin, T. and J. Han (2005) Activation and signaling of the p38 MAP kinase pathway. Cell Res. 15: 11-18. https://doi.org/10.1038/sj.cr.7290257
  10. Roux, P. P. and J. Blenis (2004) ERK and p38 MAPK-activated protein kinases: a family of protein kinases with diverse biological functions. Microbiol. Mol. Biol. Rev. 68: 320-344. https://doi.org/10.1128/MMBR.68.2.320-344.2004
  11. O'Shea, J. J., M. Gadina, and R. D. Schreiber (2002) Cell. Cytokine signaling in 2002: new surprises in the Jak/Stat pathway. 109: S121-S131. https://doi.org/10.1016/S0092-8674(02)00701-8
  12. Melnikova, V. O. and H. N. Ananthaswamy (2005) Cellular and molecular events leading to the development of skin cancer. Mutation Research 571: 91-106. https://doi.org/10.1016/j.mrfmmm.2004.11.015
  13. Matsumura Y. and H. N. Ananthaswamy (2004) Toxic effects of ultraviolet radiation on the skin. Toxicol Applied Pharmacol 195: 298-308. https://doi.org/10.1016/j.taap.2003.08.019
  14. Bode, A. M. and Z. Dong (2003) Mitogen-activated protein kinase activation in UV-induced signal transduction. Sci STKE RE2.
  15. Smith, W. L., D. L. DeWitt, and R. M. Garavito (2002) Cyclooxygenases: structural, cellular, and molecular biology. Ann. Rev. Biochem. 69: 145-182.
  16. Chen, W., Q. Tang, M. S. Gonzales, and G. T. Bowden (2001) Role of p38 MAP kinases and ERKin mediating ultraviolet-B induced cyclooxygenase-2 gene expression in human keratinocytes. Oncogene. 20: 3921-3926. https://doi.org/10.1038/sj.onc.1204530
  17. Chuda, Y., H. Ono, M. Ohnishi-Kameyama, K. Matsumoto, T. Nagata, and Y. Kikuchi (1999) Mumefural, citric acid derivative improving blood fluidity from fruit-juice concentrate of Japanese apricot (Prunus mume Sieb. et Zucc). J. Agric. Food Chem. 47: 828-831. https://doi.org/10.1021/jf980960t
  18. Kim, B. J., J. H. Kim, H. P. Kim, and M. Y. Heo (1997) Biological screening of 100 plant extracts for cosmetic use (II): anti-oxidative activity and free radical scavenging activity. Int. J. Cosmet. Sci. 19: 299-307. https://doi.org/10.1111/j.1467-2494.1997.tb00194.x
  19. Miyazawa, M., H. Utsunomiya, K. Inada, T. Yamada, Y. Okuno, H. Tanaka, and M. Tatematsu (2006) Inhibition of Helicobacter pylori motility by (+)-Syringaresinol from unripe Japanese apricot. Biol. Pharm. Bull. 29: 172-173. https://doi.org/10.1248/bpb.29.172
  20. Nakajima, S., K. Fujita, Y. Inoue, M. Nishio, and Y. Seto (2006) Effect of the folk remedy, Bainiku-ekisu, a concentrate of Prunus mume juice, on Helicobacter pylori infection in humans. Helicobacter. 11: 589-591. https://doi.org/10.1111/j.1523-5378.2006.00463.x
  21. Enomoto, S., K. Yanaoka, H. Utsunomiya, T. Niwa, K. Inada, H. Deguchi, K. Ueda, C. Mukoubayashi, I. Inoue, T. Maekita, K. Nakazawa, M. Iguchi, K. Arii, H. Tamai, N. Yoshimura, M. Fujishiro, M. Oka, and M. Ichinose (2010) Inhibitory effects of Japanese apricot (Prunus mume Siebold et Zucc.; Ume) on Helicobacter pylori-related chronic gastritis. Eur. J. Clin. Nutr. 64: 714-719. https://doi.org/10.1038/ejcn.2010.70
  22. Tsuji, R., H. Koizumi, and D. Fujiwara (2011) Effects of a plum (Prunus mume Siebold and Zucc.) ethanol extract on the immune system in vivo and in vitro. Biosci. Biotechnol. Biochem. 75: 2011-2013. https://doi.org/10.1271/bbb.100886
  23. Yu, H., D. Pardoll, and R. Jove (2009) STATs in cancer inflammation and immunity: a leading role for STAT3. Nat. Rev. Cancer 9: 798-809. https://doi.org/10.1038/nrc2734
  24. Gao, H. and P. A. Ward (2007) STAT3 and suppressor of cytokine signaling 3: potential targets in lung inflammatory responses. Expert Opin. Ther. Targets 11: 869-880. https://doi.org/10.1517/14728222.11.7.869
  25. Andrs, R. M., M. C. Montesinos, P. Navaln, M. Pay, and M. C. Terencio (2013) NF-${\kappa}B$ and STAT3 Inhibition as a Therapeutic Strategy in Psoriasis: In Vitro and In Vivo Effects of BTH. J. Invest. Dermatol. doi: 10.1038/jid.2013.182.
  26. Alessi, D. R., M. Andjelkovic, B. Caudwell, P. Cron, N. Morrice, P. Cohen, and B. A. Hemmings (1996) Mechanism of activation of protein kinase B by insulin and IGF-1. EMBO J. 15: 6541-6551.
  27. Sarbassov, D. D., D. A. Guertin, S. M. Ali, and D. M. Sabatini (2005) Phosphorylation and regulation of Akt/PKB by the rictormTOR complex. Science 307: 1098-1101. https://doi.org/10.1126/science.1106148
  28. Jacinto, E., V. Facchinetti, D. Liu, N. Soto, S. Wei, S. Y. Jung, Q. Huang, J. Qin, and B. Su (2006) SIN1/MIP1 maintains rictormTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell 127: 125-137. https://doi.org/10.1016/j.cell.2006.08.033
  29. Setlow, R. B. (1974) The wavelengths in sunlight effective in producing skin cancer: a theoretical analysis. Proc. Natl. Acad. Sci. USA 71: 3363-3366. https://doi.org/10.1073/pnas.71.9.3363
  30. Ley, R. D. (1974) Photoreactivation in humans. Proc. Natl. Acad. Sci. USA 90: 4337.
  31. Hess, J., P. Angel, and M. Schorpp-Kistner (2004) AP-1 subunits: quarrel and harmony among siblings. J. Cell. Sci. 117: 5965-5973. https://doi.org/10.1242/jcs.01589
  32. Ameyar, M., M. Wisniewska, and J. B. Weitzman (2003) A role for AP-1 in apoptosis: the case for and against. Biochimie 85: 747-752. https://doi.org/10.1016/j.biochi.2003.09.006
  33. Fischer, S. M., H. H. Lo, and G. B. Gordon (1999) Chemopreventive activity of celecoxib, a specific cyclooxygenase-2 inhibitor, and indomethacin against ultraviolet light-induced skin carcinogenesis. Mol. Carcinog. 25: 231-240. https://doi.org/10.1002/(SICI)1098-2744(199908)25:4<231::AID-MC1>3.0.CO;2-F
  34. Lee, J. L., H. Mukhtar, D. R. Bickers, L. Kopelovich, and M. Athar (2003) Cyclooxygenases in the skin: pharmacological and toxicological implications. Toxicol. Applied Pharmacol. 192: 294-306. https://doi.org/10.1016/S0041-008X(03)00301-6
  35. Karin, M. (1998) Mitogen-activated protein kinase cascades as regulators of stress responses. Ann. NY Acad. Sci. 851: 139-146. https://doi.org/10.1111/j.1749-6632.1998.tb08987.x
  36. Ding, M., R. Feng, and S. Y. Wang (2006) Cyanidin-3-glucoside, a natural product derived from blackberry, exhibits chemopreventive and chemotherapeutic activity. J. Biol. Chem. 281: 17359-17368. https://doi.org/10.1074/jbc.M600861200

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